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Yersinia enterocolitica and Yersinia pseudotuberculosis Detection in Foods
Yersinia enterocolitica and Y. pseudotuberculosis which can cause yersiniosis in humans and animals are thought to be significant food-borne pathogens and be important as hygiene indicator in food safety. The pathogenic Y. enterocolitica serotypes/biotypes are O:3/4 and 3 variant VP negative, O:5, 2...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
SAGE-Hindawi Access to Research
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3335482/ https://www.ncbi.nlm.nih.gov/pubmed/22567341 http://dx.doi.org/10.4061/2011/735308 |
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author | Fukushima, H. Shimizu, S. Inatsu, Y. |
author_facet | Fukushima, H. Shimizu, S. Inatsu, Y. |
author_sort | Fukushima, H. |
collection | PubMed |
description | Yersinia enterocolitica and Y. pseudotuberculosis which can cause yersiniosis in humans and animals are thought to be significant food-borne pathogens and be important as hygiene indicator in food safety. The pathogenic Y. enterocolitica serotypes/biotypes are O:3/4 and 3 variant VP negative, O:5, 27/2, O:8/1b, and O:9/2, have been reported worldwide. Y. pseudotuberculosis is distributed less widely than Y. enterocolitica. Isolation methods usually involve selective and recovery enrichment of the food sample followed by plating onto selective media, confirmation of typical colonies and testing for virulence properties of isolated strains. Recently, DNA-based methods, such as PCR assays, have been developed to detect pathogenic Y. enterocolitica and Y. pseudotuberculosis in foods more rapidly, and sensitivity than can be achieved by conventional culture methods. This paper reviews commercially available conventional and PCR-based procedures for the detection of pathogenic Yersinia in food. These methods are effective as the isolation and detection methods to target pathogenic Y. enterocolitica and Y. pseudotuberculosis in foods. |
format | Online Article Text |
id | pubmed-3335482 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | SAGE-Hindawi Access to Research |
record_format | MEDLINE/PubMed |
spelling | pubmed-33354822012-05-07 Yersinia enterocolitica and Yersinia pseudotuberculosis Detection in Foods Fukushima, H. Shimizu, S. Inatsu, Y. J Pathog Review Article Yersinia enterocolitica and Y. pseudotuberculosis which can cause yersiniosis in humans and animals are thought to be significant food-borne pathogens and be important as hygiene indicator in food safety. The pathogenic Y. enterocolitica serotypes/biotypes are O:3/4 and 3 variant VP negative, O:5, 27/2, O:8/1b, and O:9/2, have been reported worldwide. Y. pseudotuberculosis is distributed less widely than Y. enterocolitica. Isolation methods usually involve selective and recovery enrichment of the food sample followed by plating onto selective media, confirmation of typical colonies and testing for virulence properties of isolated strains. Recently, DNA-based methods, such as PCR assays, have been developed to detect pathogenic Y. enterocolitica and Y. pseudotuberculosis in foods more rapidly, and sensitivity than can be achieved by conventional culture methods. This paper reviews commercially available conventional and PCR-based procedures for the detection of pathogenic Yersinia in food. These methods are effective as the isolation and detection methods to target pathogenic Y. enterocolitica and Y. pseudotuberculosis in foods. SAGE-Hindawi Access to Research 2011 2011-10-05 /pmc/articles/PMC3335482/ /pubmed/22567341 http://dx.doi.org/10.4061/2011/735308 Text en Copyright © 2011 H. Fukushima et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Review Article Fukushima, H. Shimizu, S. Inatsu, Y. Yersinia enterocolitica and Yersinia pseudotuberculosis Detection in Foods |
title |
Yersinia enterocolitica and Yersinia pseudotuberculosis Detection in Foods |
title_full |
Yersinia enterocolitica and Yersinia pseudotuberculosis Detection in Foods |
title_fullStr |
Yersinia enterocolitica and Yersinia pseudotuberculosis Detection in Foods |
title_full_unstemmed |
Yersinia enterocolitica and Yersinia pseudotuberculosis Detection in Foods |
title_short |
Yersinia enterocolitica and Yersinia pseudotuberculosis Detection in Foods |
title_sort | yersinia enterocolitica and yersinia pseudotuberculosis detection in foods |
topic | Review Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3335482/ https://www.ncbi.nlm.nih.gov/pubmed/22567341 http://dx.doi.org/10.4061/2011/735308 |
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