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Expression Profiling of Cucumis sativus in Response to Infection by Pseudoperonospora cubensis

The oomycete pathogen, Pseudoperonospora cubensis, is the causal agent of downy mildew on cucurbits, and at present, no effective resistance to this pathogen is available in cultivated cucumber (Cucumis sativus). To better understand the host response to a virulent pathogen, we performed expression...

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Autores principales: Adhikari, Bishwo N., Savory, Elizabeth A., Vaillancourt, Brieanne, Childs, Kevin L., Hamilton, John P., Day, Brad, Buell, C. Robin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3335828/
https://www.ncbi.nlm.nih.gov/pubmed/22545095
http://dx.doi.org/10.1371/journal.pone.0034954
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author Adhikari, Bishwo N.
Savory, Elizabeth A.
Vaillancourt, Brieanne
Childs, Kevin L.
Hamilton, John P.
Day, Brad
Buell, C. Robin
author_facet Adhikari, Bishwo N.
Savory, Elizabeth A.
Vaillancourt, Brieanne
Childs, Kevin L.
Hamilton, John P.
Day, Brad
Buell, C. Robin
author_sort Adhikari, Bishwo N.
collection PubMed
description The oomycete pathogen, Pseudoperonospora cubensis, is the causal agent of downy mildew on cucurbits, and at present, no effective resistance to this pathogen is available in cultivated cucumber (Cucumis sativus). To better understand the host response to a virulent pathogen, we performed expression profiling throughout a time course of a compatible interaction using whole transcriptome sequencing. As described herein, we were able to detect the expression of 15,286 cucumber genes, of which 14,476 were expressed throughout the infection process from 1 day post-inoculation (dpi) to 8 dpi. A large number of genes, 1,612 to 3,286, were differentially expressed in pair-wise comparisons between time points. We observed the rapid induction of key defense related genes, including catalases, chitinases, lipoxygenases, peroxidases, and protease inhibitors within 1 dpi, suggesting detection of the pathogen by the host. Co-expression network analyses revealed transcriptional networks with distinct patterns of expression including down-regulation at 2 dpi of known defense response genes suggesting coordinated suppression of host responses by the pathogen. Comparative analyses of cucumber gene expression patterns with that of orthologous Arabidopsis thaliana genes following challenge with Hyaloperonospora arabidopsidis revealed correlated expression patterns of single copy orthologs suggesting that these two dicot hosts have similar transcriptional responses to related pathogens. In total, the work described herein presents an in-depth analysis of the interplay between host susceptibility and pathogen virulence in an agriculturally important pathosystem.
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spelling pubmed-33358282012-04-27 Expression Profiling of Cucumis sativus in Response to Infection by Pseudoperonospora cubensis Adhikari, Bishwo N. Savory, Elizabeth A. Vaillancourt, Brieanne Childs, Kevin L. Hamilton, John P. Day, Brad Buell, C. Robin PLoS One Research Article The oomycete pathogen, Pseudoperonospora cubensis, is the causal agent of downy mildew on cucurbits, and at present, no effective resistance to this pathogen is available in cultivated cucumber (Cucumis sativus). To better understand the host response to a virulent pathogen, we performed expression profiling throughout a time course of a compatible interaction using whole transcriptome sequencing. As described herein, we were able to detect the expression of 15,286 cucumber genes, of which 14,476 were expressed throughout the infection process from 1 day post-inoculation (dpi) to 8 dpi. A large number of genes, 1,612 to 3,286, were differentially expressed in pair-wise comparisons between time points. We observed the rapid induction of key defense related genes, including catalases, chitinases, lipoxygenases, peroxidases, and protease inhibitors within 1 dpi, suggesting detection of the pathogen by the host. Co-expression network analyses revealed transcriptional networks with distinct patterns of expression including down-regulation at 2 dpi of known defense response genes suggesting coordinated suppression of host responses by the pathogen. Comparative analyses of cucumber gene expression patterns with that of orthologous Arabidopsis thaliana genes following challenge with Hyaloperonospora arabidopsidis revealed correlated expression patterns of single copy orthologs suggesting that these two dicot hosts have similar transcriptional responses to related pathogens. In total, the work described herein presents an in-depth analysis of the interplay between host susceptibility and pathogen virulence in an agriculturally important pathosystem. Public Library of Science 2012-04-24 /pmc/articles/PMC3335828/ /pubmed/22545095 http://dx.doi.org/10.1371/journal.pone.0034954 Text en Adhikari et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Adhikari, Bishwo N.
Savory, Elizabeth A.
Vaillancourt, Brieanne
Childs, Kevin L.
Hamilton, John P.
Day, Brad
Buell, C. Robin
Expression Profiling of Cucumis sativus in Response to Infection by Pseudoperonospora cubensis
title Expression Profiling of Cucumis sativus in Response to Infection by Pseudoperonospora cubensis
title_full Expression Profiling of Cucumis sativus in Response to Infection by Pseudoperonospora cubensis
title_fullStr Expression Profiling of Cucumis sativus in Response to Infection by Pseudoperonospora cubensis
title_full_unstemmed Expression Profiling of Cucumis sativus in Response to Infection by Pseudoperonospora cubensis
title_short Expression Profiling of Cucumis sativus in Response to Infection by Pseudoperonospora cubensis
title_sort expression profiling of cucumis sativus in response to infection by pseudoperonospora cubensis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3335828/
https://www.ncbi.nlm.nih.gov/pubmed/22545095
http://dx.doi.org/10.1371/journal.pone.0034954
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