Cargando…

Quantitative sectioning and noise analysis for structured illumination microscopy

Structured illumination (SI) has long been regarded as a nonquantitative technique for obtaining sectioned microscopic images. Its lack of quantitative results has restricted the use of SI sectioning to qualitative imaging experiments, and has also limited researchers’ ability to compare SI against...

Descripción completa

Detalles Bibliográficos
Autores principales: Hagen, Nathan, Gao, Liang, Tkaczyk, Tomasz S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Optical Society of America 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3336372/
https://www.ncbi.nlm.nih.gov/pubmed/22274364
http://dx.doi.org/10.1364/OE.20.000403
_version_ 1782230920647933952
author Hagen, Nathan
Gao, Liang
Tkaczyk, Tomasz S.
author_facet Hagen, Nathan
Gao, Liang
Tkaczyk, Tomasz S.
author_sort Hagen, Nathan
collection PubMed
description Structured illumination (SI) has long been regarded as a nonquantitative technique for obtaining sectioned microscopic images. Its lack of quantitative results has restricted the use of SI sectioning to qualitative imaging experiments, and has also limited researchers’ ability to compare SI against competing sectioning methods such as confocal microscopy. We show how to modify the standard SI sectioning algorithm to make the technique quantitative, and provide formulas for calculating the noise in the sectioned images. The results indicate that, for an illumination source providing the same spatially-integrated photon flux at the object plane, and for the same effective slice thicknesses, SI sectioning can provide higher SNR images than confocal microscopy for an equivalent setup when the modulation contrast exceeds about 0.09.
format Online
Article
Text
id pubmed-3336372
institution National Center for Biotechnology Information
language English
publishDate 2011
publisher Optical Society of America
record_format MEDLINE/PubMed
spelling pubmed-33363722012-04-25 Quantitative sectioning and noise analysis for structured illumination microscopy Hagen, Nathan Gao, Liang Tkaczyk, Tomasz S. Opt Express Research-Article Structured illumination (SI) has long been regarded as a nonquantitative technique for obtaining sectioned microscopic images. Its lack of quantitative results has restricted the use of SI sectioning to qualitative imaging experiments, and has also limited researchers’ ability to compare SI against competing sectioning methods such as confocal microscopy. We show how to modify the standard SI sectioning algorithm to make the technique quantitative, and provide formulas for calculating the noise in the sectioned images. The results indicate that, for an illumination source providing the same spatially-integrated photon flux at the object plane, and for the same effective slice thicknesses, SI sectioning can provide higher SNR images than confocal microscopy for an equivalent setup when the modulation contrast exceeds about 0.09. Optical Society of America 2011-12-21 /pmc/articles/PMC3336372/ /pubmed/22274364 http://dx.doi.org/10.1364/OE.20.000403 Text en © 2011 Optical Society of America http://creativecommons.org/licenses/by-nc-nd/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 Unported License, which permits download and redistribution, provided that the original work is properly cited. This license restricts the article from being modified or used commercially.
spellingShingle Research-Article
Hagen, Nathan
Gao, Liang
Tkaczyk, Tomasz S.
Quantitative sectioning and noise analysis for structured illumination microscopy
title Quantitative sectioning and noise analysis for structured illumination microscopy
title_full Quantitative sectioning and noise analysis for structured illumination microscopy
title_fullStr Quantitative sectioning and noise analysis for structured illumination microscopy
title_full_unstemmed Quantitative sectioning and noise analysis for structured illumination microscopy
title_short Quantitative sectioning and noise analysis for structured illumination microscopy
title_sort quantitative sectioning and noise analysis for structured illumination microscopy
topic Research-Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3336372/
https://www.ncbi.nlm.nih.gov/pubmed/22274364
http://dx.doi.org/10.1364/OE.20.000403
work_keys_str_mv AT hagennathan quantitativesectioningandnoiseanalysisforstructuredilluminationmicroscopy
AT gaoliang quantitativesectioningandnoiseanalysisforstructuredilluminationmicroscopy
AT tkaczyktomaszs quantitativesectioningandnoiseanalysisforstructuredilluminationmicroscopy