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Quantitative sectioning and noise analysis for structured illumination microscopy
Structured illumination (SI) has long been regarded as a nonquantitative technique for obtaining sectioned microscopic images. Its lack of quantitative results has restricted the use of SI sectioning to qualitative imaging experiments, and has also limited researchers’ ability to compare SI against...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Optical Society of America
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3336372/ https://www.ncbi.nlm.nih.gov/pubmed/22274364 http://dx.doi.org/10.1364/OE.20.000403 |
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author | Hagen, Nathan Gao, Liang Tkaczyk, Tomasz S. |
author_facet | Hagen, Nathan Gao, Liang Tkaczyk, Tomasz S. |
author_sort | Hagen, Nathan |
collection | PubMed |
description | Structured illumination (SI) has long been regarded as a nonquantitative technique for obtaining sectioned microscopic images. Its lack of quantitative results has restricted the use of SI sectioning to qualitative imaging experiments, and has also limited researchers’ ability to compare SI against competing sectioning methods such as confocal microscopy. We show how to modify the standard SI sectioning algorithm to make the technique quantitative, and provide formulas for calculating the noise in the sectioned images. The results indicate that, for an illumination source providing the same spatially-integrated photon flux at the object plane, and for the same effective slice thicknesses, SI sectioning can provide higher SNR images than confocal microscopy for an equivalent setup when the modulation contrast exceeds about 0.09. |
format | Online Article Text |
id | pubmed-3336372 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Optical Society of America |
record_format | MEDLINE/PubMed |
spelling | pubmed-33363722012-04-25 Quantitative sectioning and noise analysis for structured illumination microscopy Hagen, Nathan Gao, Liang Tkaczyk, Tomasz S. Opt Express Research-Article Structured illumination (SI) has long been regarded as a nonquantitative technique for obtaining sectioned microscopic images. Its lack of quantitative results has restricted the use of SI sectioning to qualitative imaging experiments, and has also limited researchers’ ability to compare SI against competing sectioning methods such as confocal microscopy. We show how to modify the standard SI sectioning algorithm to make the technique quantitative, and provide formulas for calculating the noise in the sectioned images. The results indicate that, for an illumination source providing the same spatially-integrated photon flux at the object plane, and for the same effective slice thicknesses, SI sectioning can provide higher SNR images than confocal microscopy for an equivalent setup when the modulation contrast exceeds about 0.09. Optical Society of America 2011-12-21 /pmc/articles/PMC3336372/ /pubmed/22274364 http://dx.doi.org/10.1364/OE.20.000403 Text en © 2011 Optical Society of America http://creativecommons.org/licenses/by-nc-nd/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 Unported License, which permits download and redistribution, provided that the original work is properly cited. This license restricts the article from being modified or used commercially. |
spellingShingle | Research-Article Hagen, Nathan Gao, Liang Tkaczyk, Tomasz S. Quantitative sectioning and noise analysis for structured illumination microscopy |
title | Quantitative sectioning and noise analysis for structured illumination microscopy |
title_full | Quantitative sectioning and noise analysis for structured illumination microscopy |
title_fullStr | Quantitative sectioning and noise analysis for structured illumination microscopy |
title_full_unstemmed | Quantitative sectioning and noise analysis for structured illumination microscopy |
title_short | Quantitative sectioning and noise analysis for structured illumination microscopy |
title_sort | quantitative sectioning and noise analysis for structured illumination microscopy |
topic | Research-Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3336372/ https://www.ncbi.nlm.nih.gov/pubmed/22274364 http://dx.doi.org/10.1364/OE.20.000403 |
work_keys_str_mv | AT hagennathan quantitativesectioningandnoiseanalysisforstructuredilluminationmicroscopy AT gaoliang quantitativesectioningandnoiseanalysisforstructuredilluminationmicroscopy AT tkaczyktomaszs quantitativesectioningandnoiseanalysisforstructuredilluminationmicroscopy |