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A high-throughput FRET-based assay for determination of Atg4 activity

Atg4 is required for cleaving Atg8, allowing it to be conjugated to phosphatidylethanolamine on phagophore membranes, a key step in autophagosome biogenesis. Deconjugation of Atg8 from autophagosomal membranes could be also a regulatory step in controlling autophagy. Therefore, the activity of Atg4...

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Detalles Bibliográficos
Autores principales:	Li, Min, Chen, Xi, Ye, Qi-Zhuang, Vogt, Andreas, Yin, Xiao-Ming
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Landes Bioscience 2012
Materias:	Toolbox
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3337841/ https://www.ncbi.nlm.nih.gov/pubmed/22302004 http://dx.doi.org/10.4161/auto.18777

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