Construction of shRNA lentiviral vector

Lentiviruses have been adapted as gene delivery vehicles. This article summarized shRNA lentiviral vector methods generally used in research laboratories. The main procedures of shRNA lentiviral vector include that (1) Target sequences screening and shRNA oligonucleotides designing, (2) insert desig...

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Detalles Bibliográficos
Autores principales: Song, Hong, Yang, Ping-Chang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2010
Materias:
Technical Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3338230/
https://www.ncbi.nlm.nih.gov/pubmed/22558575
http://dx.doi.org/10.4297/najms.2010.2598
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author Song, Hong
Yang, Ping-Chang
author_facet Song, Hong
Yang, Ping-Chang
author_sort Song, Hong
collection PubMed
description Lentiviruses have been adapted as gene delivery vehicles. This article summarized shRNA lentiviral vector methods generally used in research laboratories. The main procedures of shRNA lentiviral vector include that (1) Target sequences screening and shRNA oligonucleotides designing, (2) insert designed oligonucleotides into lentiviral vectors, (3) using packaging cells to produce shRNA lentivirus, and (4) transducing target cells with shRNA lentivirus.
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spelling pubmed-33382302012-05-03 Construction of shRNA lentiviral vector Song, Hong Yang, Ping-Chang N Am J Med Sci Technical Article Lentiviruses have been adapted as gene delivery vehicles. This article summarized shRNA lentiviral vector methods generally used in research laboratories. The main procedures of shRNA lentiviral vector include that (1) Target sequences screening and shRNA oligonucleotides designing, (2) insert designed oligonucleotides into lentiviral vectors, (3) using packaging cells to produce shRNA lentivirus, and (4) transducing target cells with shRNA lentivirus. Medknow Publications & Media Pvt Ltd 2010-12 /pmc/articles/PMC3338230/ /pubmed/22558575 http://dx.doi.org/10.4297/najms.2010.2598 Text en Copyright: © North American Journal of Medical Sciences http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Technical Article
Song, Hong
Yang, Ping-Chang
Construction of shRNA lentiviral vector
title Construction of shRNA lentiviral vector
title_full Construction of shRNA lentiviral vector
title_fullStr Construction of shRNA lentiviral vector
title_full_unstemmed Construction of shRNA lentiviral vector
title_short Construction of shRNA lentiviral vector
title_sort construction of shrna lentiviral vector
topic Technical Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3338230/
https://www.ncbi.nlm.nih.gov/pubmed/22558575
http://dx.doi.org/10.4297/najms.2010.2598
work_keys_str_mv AT songhong constructionofshrnalentiviralvector
AT yangpingchang constructionofshrnalentiviralvector

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