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Identification of IGPR-1 as a novel adhesion molecule involved in angiogenesis

Angiogenesis—the growth of new blood vessels from preexisting vessels—is an important physiological process and is considered to play a key role in tumor growth and metastasis. We identified the immunoglobulin-containing and proline-rich receptor-1 (IGPR-1, also called TMIGD2) gene as a novel cell a...

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Autores principales: Rahimi, Nader, Rezazadeh, Kobra, Mahoney, John E., Hartsough, Edward, Meyer, Rosana D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3338432/
https://www.ncbi.nlm.nih.gov/pubmed/22419821
http://dx.doi.org/10.1091/mbc.E11-11-0934
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author Rahimi, Nader
Rezazadeh, Kobra
Mahoney, John E.
Hartsough, Edward
Meyer, Rosana D.
author_facet Rahimi, Nader
Rezazadeh, Kobra
Mahoney, John E.
Hartsough, Edward
Meyer, Rosana D.
author_sort Rahimi, Nader
collection PubMed
description Angiogenesis—the growth of new blood vessels from preexisting vessels—is an important physiological process and is considered to play a key role in tumor growth and metastasis. We identified the immunoglobulin-containing and proline-rich receptor-1 (IGPR-1, also called TMIGD2) gene as a novel cell adhesion receptor that is expressed in various human organs and tissues, mainly in cells with epithelium and endothelium origins. IGPR-1 regulates cellular morphology, homophilic cell aggregation, and cell–cell interaction. IGPR-1 activity also modulates actin stress fiber formation and focal adhesion and reduces cell migration. Silencing of expression of IGPR-1 by small interfering RNA (siRNA) and by ectopic overexpression in endothelial cells showed that IGPR-1 regulates capillary tube formation in vitro, and B16F melanoma cells engineered to express IGPR-1 displayed extensive angiogenesis in the mouse Matrigel angiogenesis model. Moreover, IGPR-1, through its proline-rich cytoplasmic domain, associates with multiple Src homology 3 (SH3)–containing signaling proteins, including SH3 protein interacting with Nck (SPIN90/WISH), bullous pemphigoid antigen-1, and calcium channel β2. Silencing of expression of SPIN90/WISH by siRNA in endothelial cells showed that SPIN90/WISH is required for capillary tube formation. These features of IGPR-1 suggest that IGPR-1 is a novel receptor that plays an important role in cell–cell interaction, cell migration, and angiogenesis.
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spelling pubmed-33384322012-07-16 Identification of IGPR-1 as a novel adhesion molecule involved in angiogenesis Rahimi, Nader Rezazadeh, Kobra Mahoney, John E. Hartsough, Edward Meyer, Rosana D. Mol Biol Cell Articles Angiogenesis—the growth of new blood vessels from preexisting vessels—is an important physiological process and is considered to play a key role in tumor growth and metastasis. We identified the immunoglobulin-containing and proline-rich receptor-1 (IGPR-1, also called TMIGD2) gene as a novel cell adhesion receptor that is expressed in various human organs and tissues, mainly in cells with epithelium and endothelium origins. IGPR-1 regulates cellular morphology, homophilic cell aggregation, and cell–cell interaction. IGPR-1 activity also modulates actin stress fiber formation and focal adhesion and reduces cell migration. Silencing of expression of IGPR-1 by small interfering RNA (siRNA) and by ectopic overexpression in endothelial cells showed that IGPR-1 regulates capillary tube formation in vitro, and B16F melanoma cells engineered to express IGPR-1 displayed extensive angiogenesis in the mouse Matrigel angiogenesis model. Moreover, IGPR-1, through its proline-rich cytoplasmic domain, associates with multiple Src homology 3 (SH3)–containing signaling proteins, including SH3 protein interacting with Nck (SPIN90/WISH), bullous pemphigoid antigen-1, and calcium channel β2. Silencing of expression of SPIN90/WISH by siRNA in endothelial cells showed that SPIN90/WISH is required for capillary tube formation. These features of IGPR-1 suggest that IGPR-1 is a novel receptor that plays an important role in cell–cell interaction, cell migration, and angiogenesis. The American Society for Cell Biology 2012-05-01 /pmc/articles/PMC3338432/ /pubmed/22419821 http://dx.doi.org/10.1091/mbc.E11-11-0934 Text en © 2012 Rahimi et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License(http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society of Cell Biology.
spellingShingle Articles
Rahimi, Nader
Rezazadeh, Kobra
Mahoney, John E.
Hartsough, Edward
Meyer, Rosana D.
Identification of IGPR-1 as a novel adhesion molecule involved in angiogenesis
title Identification of IGPR-1 as a novel adhesion molecule involved in angiogenesis
title_full Identification of IGPR-1 as a novel adhesion molecule involved in angiogenesis
title_fullStr Identification of IGPR-1 as a novel adhesion molecule involved in angiogenesis
title_full_unstemmed Identification of IGPR-1 as a novel adhesion molecule involved in angiogenesis
title_short Identification of IGPR-1 as a novel adhesion molecule involved in angiogenesis
title_sort identification of igpr-1 as a novel adhesion molecule involved in angiogenesis
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3338432/
https://www.ncbi.nlm.nih.gov/pubmed/22419821
http://dx.doi.org/10.1091/mbc.E11-11-0934
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