Cargando…
Axonal and dendritic synaptotagmin isoforms revealed by a pHluorin-syt functional screen
The synaptotagmins (syts) are a family of molecules that regulate membrane fusion. There are 17 mammalian syt isoforms, most of which are expressed in the brain. However, little is known regarding the subcellular location and function of the majority of these syts in neurons, largely due to a lack o...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The American Society for Cell Biology
2012
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3338438/ https://www.ncbi.nlm.nih.gov/pubmed/22398727 http://dx.doi.org/10.1091/mbc.E11-08-0707 |
_version_ | 1782231189915959296 |
---|---|
author | Dean, Camin Dunning, F. Mark Liu, Huisheng Bomba-Warczak, Ewa Martens, Henrik Bharat, Vinita Ahmed, Saheeb Chapman, Edwin R. |
author_facet | Dean, Camin Dunning, F. Mark Liu, Huisheng Bomba-Warczak, Ewa Martens, Henrik Bharat, Vinita Ahmed, Saheeb Chapman, Edwin R. |
author_sort | Dean, Camin |
collection | PubMed |
description | The synaptotagmins (syts) are a family of molecules that regulate membrane fusion. There are 17 mammalian syt isoforms, most of which are expressed in the brain. However, little is known regarding the subcellular location and function of the majority of these syts in neurons, largely due to a lack of isoform-specific antibodies. Here we generated pHluorin-syt constructs harboring a luminal domain pH sensor, which reports localization, pH of organelles to which syts are targeted, and the kinetics and sites of exocytosis and endocytosis. Of interest, only syt-1 and 2 are targeted to synaptic vesicles, whereas other isoforms selectively recycle in dendrites (syt-3 and 11), axons (syt-5, 7, 10, and 17), or both axons and dendrites (syt-4, 6, 9, and 12), where they undergo exocytosis and endocytosis with distinctive kinetics. Hence most syt isoforms localize to distinct secretory organelles in both axons and dendrites and may regulate neuropeptide/neurotrophin release to modulate neuronal function. |
format | Online Article Text |
id | pubmed-3338438 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | The American Society for Cell Biology |
record_format | MEDLINE/PubMed |
spelling | pubmed-33384382012-07-16 Axonal and dendritic synaptotagmin isoforms revealed by a pHluorin-syt functional screen Dean, Camin Dunning, F. Mark Liu, Huisheng Bomba-Warczak, Ewa Martens, Henrik Bharat, Vinita Ahmed, Saheeb Chapman, Edwin R. Mol Biol Cell Articles The synaptotagmins (syts) are a family of molecules that regulate membrane fusion. There are 17 mammalian syt isoforms, most of which are expressed in the brain. However, little is known regarding the subcellular location and function of the majority of these syts in neurons, largely due to a lack of isoform-specific antibodies. Here we generated pHluorin-syt constructs harboring a luminal domain pH sensor, which reports localization, pH of organelles to which syts are targeted, and the kinetics and sites of exocytosis and endocytosis. Of interest, only syt-1 and 2 are targeted to synaptic vesicles, whereas other isoforms selectively recycle in dendrites (syt-3 and 11), axons (syt-5, 7, 10, and 17), or both axons and dendrites (syt-4, 6, 9, and 12), where they undergo exocytosis and endocytosis with distinctive kinetics. Hence most syt isoforms localize to distinct secretory organelles in both axons and dendrites and may regulate neuropeptide/neurotrophin release to modulate neuronal function. The American Society for Cell Biology 2012-05-01 /pmc/articles/PMC3338438/ /pubmed/22398727 http://dx.doi.org/10.1091/mbc.E11-08-0707 Text en © 2012 Dean et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society of Cell Biology. |
spellingShingle | Articles Dean, Camin Dunning, F. Mark Liu, Huisheng Bomba-Warczak, Ewa Martens, Henrik Bharat, Vinita Ahmed, Saheeb Chapman, Edwin R. Axonal and dendritic synaptotagmin isoforms revealed by a pHluorin-syt functional screen |
title | Axonal and dendritic synaptotagmin isoforms revealed by a pHluorin-syt functional screen |
title_full | Axonal and dendritic synaptotagmin isoforms revealed by a pHluorin-syt functional screen |
title_fullStr | Axonal and dendritic synaptotagmin isoforms revealed by a pHluorin-syt functional screen |
title_full_unstemmed | Axonal and dendritic synaptotagmin isoforms revealed by a pHluorin-syt functional screen |
title_short | Axonal and dendritic synaptotagmin isoforms revealed by a pHluorin-syt functional screen |
title_sort | axonal and dendritic synaptotagmin isoforms revealed by a phluorin-syt functional screen |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3338438/ https://www.ncbi.nlm.nih.gov/pubmed/22398727 http://dx.doi.org/10.1091/mbc.E11-08-0707 |
work_keys_str_mv | AT deancamin axonalanddendriticsynaptotagminisoformsrevealedbyaphluorinsytfunctionalscreen AT dunningfmark axonalanddendriticsynaptotagminisoformsrevealedbyaphluorinsytfunctionalscreen AT liuhuisheng axonalanddendriticsynaptotagminisoformsrevealedbyaphluorinsytfunctionalscreen AT bombawarczakewa axonalanddendriticsynaptotagminisoformsrevealedbyaphluorinsytfunctionalscreen AT martenshenrik axonalanddendriticsynaptotagminisoformsrevealedbyaphluorinsytfunctionalscreen AT bharatvinita axonalanddendriticsynaptotagminisoformsrevealedbyaphluorinsytfunctionalscreen AT ahmedsaheeb axonalanddendriticsynaptotagminisoformsrevealedbyaphluorinsytfunctionalscreen AT chapmanedwinr axonalanddendriticsynaptotagminisoformsrevealedbyaphluorinsytfunctionalscreen |