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Identification of a novel ADAMTS9/GON-1 function for protein transport from the ER to the Golgi

A disintegrin-like and metalloprotease with thrombospondin type I motif (ADAMTS9) is a member of the secreted metalloprotease family that is believed to digest extracellular matrix (ECM) proteins outside of cells. Its Caenorhabditis elegans orthologue, GON-1, is involved in ECM degradation and is re...

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Autores principales: Yoshina, Sawako, Sakaki, Kenjiro, Yonezumi-Hayashi, Aki, Gengyo-Ando, Keiko, Inoue, Hideshi, Iino, Yuichi, Mitani, Shohei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3338439/
https://www.ncbi.nlm.nih.gov/pubmed/22419820
http://dx.doi.org/10.1091/mbc.E11-10-0857
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author Yoshina, Sawako
Sakaki, Kenjiro
Yonezumi-Hayashi, Aki
Gengyo-Ando, Keiko
Inoue, Hideshi
Iino, Yuichi
Mitani, Shohei
author_facet Yoshina, Sawako
Sakaki, Kenjiro
Yonezumi-Hayashi, Aki
Gengyo-Ando, Keiko
Inoue, Hideshi
Iino, Yuichi
Mitani, Shohei
author_sort Yoshina, Sawako
collection PubMed
description A disintegrin-like and metalloprotease with thrombospondin type I motif (ADAMTS9) is a member of the secreted metalloprotease family that is believed to digest extracellular matrix (ECM) proteins outside of cells. Its Caenorhabditis elegans orthologue, GON-1, is involved in ECM degradation and is required for gonad morphogenesis. ADAMTS9 and GON-1 have similar domain structures, and both have a unique C-terminal domain called the “GON domain,” whose function remains unknown. Here we show that down-regulation of human ADAMTS9 and C. elegans GON-1 results in the inhibition of protein transport from the endoplasmic reticulum (ER) to the Golgi. This phenotype was rescued by the expression of the GON domain localizing in the ER in human cells and C. elegans. We propose a novel function of ADAMTS9 and GON-1 in the ER that promotes protein transport from the ER to the Golgi. This function is GON-domain dependent but protease activity independent.
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spelling pubmed-33384392012-07-16 Identification of a novel ADAMTS9/GON-1 function for protein transport from the ER to the Golgi Yoshina, Sawako Sakaki, Kenjiro Yonezumi-Hayashi, Aki Gengyo-Ando, Keiko Inoue, Hideshi Iino, Yuichi Mitani, Shohei Mol Biol Cell Articles A disintegrin-like and metalloprotease with thrombospondin type I motif (ADAMTS9) is a member of the secreted metalloprotease family that is believed to digest extracellular matrix (ECM) proteins outside of cells. Its Caenorhabditis elegans orthologue, GON-1, is involved in ECM degradation and is required for gonad morphogenesis. ADAMTS9 and GON-1 have similar domain structures, and both have a unique C-terminal domain called the “GON domain,” whose function remains unknown. Here we show that down-regulation of human ADAMTS9 and C. elegans GON-1 results in the inhibition of protein transport from the endoplasmic reticulum (ER) to the Golgi. This phenotype was rescued by the expression of the GON domain localizing in the ER in human cells and C. elegans. We propose a novel function of ADAMTS9 and GON-1 in the ER that promotes protein transport from the ER to the Golgi. This function is GON-domain dependent but protease activity independent. The American Society for Cell Biology 2012-05-01 /pmc/articles/PMC3338439/ /pubmed/22419820 http://dx.doi.org/10.1091/mbc.E11-10-0857 Text en © 2012 Yoshina et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society of Cell Biology.
spellingShingle Articles
Yoshina, Sawako
Sakaki, Kenjiro
Yonezumi-Hayashi, Aki
Gengyo-Ando, Keiko
Inoue, Hideshi
Iino, Yuichi
Mitani, Shohei
Identification of a novel ADAMTS9/GON-1 function for protein transport from the ER to the Golgi
title Identification of a novel ADAMTS9/GON-1 function for protein transport from the ER to the Golgi
title_full Identification of a novel ADAMTS9/GON-1 function for protein transport from the ER to the Golgi
title_fullStr Identification of a novel ADAMTS9/GON-1 function for protein transport from the ER to the Golgi
title_full_unstemmed Identification of a novel ADAMTS9/GON-1 function for protein transport from the ER to the Golgi
title_short Identification of a novel ADAMTS9/GON-1 function for protein transport from the ER to the Golgi
title_sort identification of a novel adamts9/gon-1 function for protein transport from the er to the golgi
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3338439/
https://www.ncbi.nlm.nih.gov/pubmed/22419820
http://dx.doi.org/10.1091/mbc.E11-10-0857
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