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A Sulfhydryl-Reactive Ruthenium (II) Complex and Its Conjugation to Protein G as a Universal Reagent for Fluorescent Immunoassays

To develop a fluorescent ruthenium complex for biosensing, we synthesized a novel sulfhydryl-reactive compound, 4-bromophenanthroline bis-2,2′-dipyridine Ruthenium bis (hexafluorophosphate). The synthesized Ru(II) complex was crosslinked with thiol-modified protein G to form a universal reagent for...

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Detalles Bibliográficos
Autores principales:	Lin, Jing-Tang, Chen, Po-Chung, Goud, Thirumani Venkatshwar, Huang, Bor-Rong, Lin, Tzu-Chau, Biellmann, Jean-François, Chen, Chien-Sheng
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2012
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3338566/ https://www.ncbi.nlm.nih.gov/pubmed/22563441 http://dx.doi.org/10.1371/journal.pone.0036086

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