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Nervous Necrosis Virus Replicates Following the Embryo Development and Dual Infection with Iridovirus at Juvenile Stage in Grouper

Infection of virus (such as nodavirus and iridovirus) and bacteria (such as Vibrio anguillarum) in farmed grouper has been widely reported and caused large economic losses to Taiwanese fish aquaculture industry since 1979. The multiplex assay was used to detect dual viral infection and showed that o...

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Autores principales: Kuo, Hsiao-Che, Wang, Ting-Yu, Hsu, Hao-Hsuan, Chen, Peng-Peng, Lee, Szu-Hsien, Chen, Young-Mao, Tsai, Tieh-Jung, Wang, Chien-Kai, Ku, Hsiao-Tung, Lee, Gwo-Bin, Chen, Tzong-Yueh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3338570/
https://www.ncbi.nlm.nih.gov/pubmed/22563447
http://dx.doi.org/10.1371/journal.pone.0036183
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author Kuo, Hsiao-Che
Wang, Ting-Yu
Hsu, Hao-Hsuan
Chen, Peng-Peng
Lee, Szu-Hsien
Chen, Young-Mao
Tsai, Tieh-Jung
Wang, Chien-Kai
Ku, Hsiao-Tung
Lee, Gwo-Bin
Chen, Tzong-Yueh
author_facet Kuo, Hsiao-Che
Wang, Ting-Yu
Hsu, Hao-Hsuan
Chen, Peng-Peng
Lee, Szu-Hsien
Chen, Young-Mao
Tsai, Tieh-Jung
Wang, Chien-Kai
Ku, Hsiao-Tung
Lee, Gwo-Bin
Chen, Tzong-Yueh
author_sort Kuo, Hsiao-Che
collection PubMed
description Infection of virus (such as nodavirus and iridovirus) and bacteria (such as Vibrio anguillarum) in farmed grouper has been widely reported and caused large economic losses to Taiwanese fish aquaculture industry since 1979. The multiplex assay was used to detect dual viral infection and showed that only nervous necrosis virus (NNV) can be detected till the end of experiments (100% mortality) once it appeared. In addition, iridovirus can be detected in a certain period of rearing. The results of real-time PCR and in situ PCR indicated that NNV, in fact, was not on the surface of the eggs but present in the embryo, which can continue to replicate during the embryo development. The virus may be vertically transmitted by packing into eggs during egg development (formation) or delivering into eggs by sperm during fertilization. The ozone treatment of eggs may fail to remove the virus, so a new strategy to prevent NNV is needed.
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spelling pubmed-33385702012-05-04 Nervous Necrosis Virus Replicates Following the Embryo Development and Dual Infection with Iridovirus at Juvenile Stage in Grouper Kuo, Hsiao-Che Wang, Ting-Yu Hsu, Hao-Hsuan Chen, Peng-Peng Lee, Szu-Hsien Chen, Young-Mao Tsai, Tieh-Jung Wang, Chien-Kai Ku, Hsiao-Tung Lee, Gwo-Bin Chen, Tzong-Yueh PLoS One Research Article Infection of virus (such as nodavirus and iridovirus) and bacteria (such as Vibrio anguillarum) in farmed grouper has been widely reported and caused large economic losses to Taiwanese fish aquaculture industry since 1979. The multiplex assay was used to detect dual viral infection and showed that only nervous necrosis virus (NNV) can be detected till the end of experiments (100% mortality) once it appeared. In addition, iridovirus can be detected in a certain period of rearing. The results of real-time PCR and in situ PCR indicated that NNV, in fact, was not on the surface of the eggs but present in the embryo, which can continue to replicate during the embryo development. The virus may be vertically transmitted by packing into eggs during egg development (formation) or delivering into eggs by sperm during fertilization. The ozone treatment of eggs may fail to remove the virus, so a new strategy to prevent NNV is needed. Public Library of Science 2012-04-26 /pmc/articles/PMC3338570/ /pubmed/22563447 http://dx.doi.org/10.1371/journal.pone.0036183 Text en Kuo et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Kuo, Hsiao-Che
Wang, Ting-Yu
Hsu, Hao-Hsuan
Chen, Peng-Peng
Lee, Szu-Hsien
Chen, Young-Mao
Tsai, Tieh-Jung
Wang, Chien-Kai
Ku, Hsiao-Tung
Lee, Gwo-Bin
Chen, Tzong-Yueh
Nervous Necrosis Virus Replicates Following the Embryo Development and Dual Infection with Iridovirus at Juvenile Stage in Grouper
title Nervous Necrosis Virus Replicates Following the Embryo Development and Dual Infection with Iridovirus at Juvenile Stage in Grouper
title_full Nervous Necrosis Virus Replicates Following the Embryo Development and Dual Infection with Iridovirus at Juvenile Stage in Grouper
title_fullStr Nervous Necrosis Virus Replicates Following the Embryo Development and Dual Infection with Iridovirus at Juvenile Stage in Grouper
title_full_unstemmed Nervous Necrosis Virus Replicates Following the Embryo Development and Dual Infection with Iridovirus at Juvenile Stage in Grouper
title_short Nervous Necrosis Virus Replicates Following the Embryo Development and Dual Infection with Iridovirus at Juvenile Stage in Grouper
title_sort nervous necrosis virus replicates following the embryo development and dual infection with iridovirus at juvenile stage in grouper
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3338570/
https://www.ncbi.nlm.nih.gov/pubmed/22563447
http://dx.doi.org/10.1371/journal.pone.0036183
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