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Live Imaging of Mitosomes and Hydrogenosomes by HaloTag Technology
Hydrogenosomes and mitosomes represent remarkable mitochondrial adaptations in the anaerobic parasitic protists such as Trichomonas vaginalis and Giardia intestinalis, respectively. In order to provide a tool to study these organelles in the live cells, the HaloTag was fused to G. intestinalis IscU...
| Autores principales: | , , , , , , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Public Library of Science
2012
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3338651/ https://www.ncbi.nlm.nih.gov/pubmed/22558433 http://dx.doi.org/10.1371/journal.pone.0036314 |
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| author | Martincová, Eva Voleman, Luboš Najdrová, Vladimíra De Napoli, Maximiliano Eshar, Shiri Gualdron, Melisa Hopp, Christine S. Sanin, David E. Tembo, Dumizulu L. Van Tyne, Daria Walker, Dawn Marcinčiková, Michaela Tachezy, Jan Doležal, Pavel |
| author_facet | Martincová, Eva Voleman, Luboš Najdrová, Vladimíra De Napoli, Maximiliano Eshar, Shiri Gualdron, Melisa Hopp, Christine S. Sanin, David E. Tembo, Dumizulu L. Van Tyne, Daria Walker, Dawn Marcinčiková, Michaela Tachezy, Jan Doležal, Pavel |
| author_sort | Martincová, Eva |
| collection | PubMed |
| description | Hydrogenosomes and mitosomes represent remarkable mitochondrial adaptations in the anaerobic parasitic protists such as Trichomonas vaginalis and Giardia intestinalis, respectively. In order to provide a tool to study these organelles in the live cells, the HaloTag was fused to G. intestinalis IscU and T. vaginalis frataxin and expressed in the mitosomes and hydrogenosomes, respectively. The incubation of the parasites with the fluorescent Halo-ligand resulted in highly specific organellar labeling, allowing live imaging of the organelles. With the array of available ligands the HaloTag technology offers a new tool to study the dynamics of mitochondria-related compartments as well as other cellular components in these intriguing unicellular eukaryotes. |
| format | Online Article Text |
| id | pubmed-3338651 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2012 |
| publisher | Public Library of Science |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-33386512012-05-03 Live Imaging of Mitosomes and Hydrogenosomes by HaloTag Technology Martincová, Eva Voleman, Luboš Najdrová, Vladimíra De Napoli, Maximiliano Eshar, Shiri Gualdron, Melisa Hopp, Christine S. Sanin, David E. Tembo, Dumizulu L. Van Tyne, Daria Walker, Dawn Marcinčiková, Michaela Tachezy, Jan Doležal, Pavel PLoS One Research Article Hydrogenosomes and mitosomes represent remarkable mitochondrial adaptations in the anaerobic parasitic protists such as Trichomonas vaginalis and Giardia intestinalis, respectively. In order to provide a tool to study these organelles in the live cells, the HaloTag was fused to G. intestinalis IscU and T. vaginalis frataxin and expressed in the mitosomes and hydrogenosomes, respectively. The incubation of the parasites with the fluorescent Halo-ligand resulted in highly specific organellar labeling, allowing live imaging of the organelles. With the array of available ligands the HaloTag technology offers a new tool to study the dynamics of mitochondria-related compartments as well as other cellular components in these intriguing unicellular eukaryotes. Public Library of Science 2012-04-27 /pmc/articles/PMC3338651/ /pubmed/22558433 http://dx.doi.org/10.1371/journal.pone.0036314 Text en Martincová et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
| spellingShingle | Research Article Martincová, Eva Voleman, Luboš Najdrová, Vladimíra De Napoli, Maximiliano Eshar, Shiri Gualdron, Melisa Hopp, Christine S. Sanin, David E. Tembo, Dumizulu L. Van Tyne, Daria Walker, Dawn Marcinčiková, Michaela Tachezy, Jan Doležal, Pavel Live Imaging of Mitosomes and Hydrogenosomes by HaloTag Technology |
| title | Live Imaging of Mitosomes and Hydrogenosomes by HaloTag Technology |
| title_full | Live Imaging of Mitosomes and Hydrogenosomes by HaloTag Technology |
| title_fullStr | Live Imaging of Mitosomes and Hydrogenosomes by HaloTag Technology |
| title_full_unstemmed | Live Imaging of Mitosomes and Hydrogenosomes by HaloTag Technology |
| title_short | Live Imaging of Mitosomes and Hydrogenosomes by HaloTag Technology |
| title_sort | live imaging of mitosomes and hydrogenosomes by halotag technology |
| topic | Research Article |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3338651/ https://www.ncbi.nlm.nih.gov/pubmed/22558433 http://dx.doi.org/10.1371/journal.pone.0036314 |
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