Glutaraldehyde Treatment Elicits Toxic Response Compared to Decellularization in Bovine Pericardium

Glutaraldehyde-stabilized bovine pericardium is used for clinical application since 1970s because of its desirable features such as less immunogenicity and acceptable durability. However, a propensity for calcification is reported on account of glutaraldehyde treatment. In this study, commercially a...

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Autores principales: Umashankar, P.R., Mohanan, P.V., Kumari, T.V.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3339246/
https://www.ncbi.nlm.nih.gov/pubmed/22736904
http://dx.doi.org/10.4103/0971-6580.94513
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author Umashankar, P.R.
Mohanan, P.V.
Kumari, T.V.
author_facet Umashankar, P.R.
Mohanan, P.V.
Kumari, T.V.
author_sort Umashankar, P.R.
collection PubMed
description Glutaraldehyde-stabilized bovine pericardium is used for clinical application since 1970s because of its desirable features such as less immunogenicity and acceptable durability. However, a propensity for calcification is reported on account of glutaraldehyde treatment. In this study, commercially available glutaraldehyde cross-linked bovine pericardium was evaluated for its in vitro cytotoxic effect, macrophage activation, and in vivo toxic response in comparison to decellularized bovine pericardium. Glutaraldehyde-treated bovine pericardium and its extract were observed to be cytotoxic and it also caused significant inflammatory cytokine release from activated macrophages. Significant antibody response, calcification response, necrotic, and inflammatory response were noticed in glutaraldehyde-treated bovine pericardium in comparison to decellularized bovine pericardium in a rat subcutaneous implantation model. Glutaraldehyde-treated bovine pericardium also failed in acute systemic toxicity testing and intracutaneous irritation testing as per ISO 10993. With respect to healing and implant remodeling, total lack of host tissue incorporation and angiogenesis was noticed in glutaraldehyde-treated bovine pericardium compared to excellent host fibroblast incorporation and angiogenesis within the implant in decellularized bovine pericardium. In conclusion, using in vitro and in vivo techniques, this study has demonstrated that glutaraldehyde-treated bovine pericardium elicits toxic response compared to decellularized bovine pericardium which is not congenial for long-term implant performance.
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spelling pubmed-33392462012-06-25 Glutaraldehyde Treatment Elicits Toxic Response Compared to Decellularization in Bovine Pericardium Umashankar, P.R. Mohanan, P.V. Kumari, T.V. Toxicol Int Original Article Glutaraldehyde-stabilized bovine pericardium is used for clinical application since 1970s because of its desirable features such as less immunogenicity and acceptable durability. However, a propensity for calcification is reported on account of glutaraldehyde treatment. In this study, commercially available glutaraldehyde cross-linked bovine pericardium was evaluated for its in vitro cytotoxic effect, macrophage activation, and in vivo toxic response in comparison to decellularized bovine pericardium. Glutaraldehyde-treated bovine pericardium and its extract were observed to be cytotoxic and it also caused significant inflammatory cytokine release from activated macrophages. Significant antibody response, calcification response, necrotic, and inflammatory response were noticed in glutaraldehyde-treated bovine pericardium in comparison to decellularized bovine pericardium in a rat subcutaneous implantation model. Glutaraldehyde-treated bovine pericardium also failed in acute systemic toxicity testing and intracutaneous irritation testing as per ISO 10993. With respect to healing and implant remodeling, total lack of host tissue incorporation and angiogenesis was noticed in glutaraldehyde-treated bovine pericardium compared to excellent host fibroblast incorporation and angiogenesis within the implant in decellularized bovine pericardium. In conclusion, using in vitro and in vivo techniques, this study has demonstrated that glutaraldehyde-treated bovine pericardium elicits toxic response compared to decellularized bovine pericardium which is not congenial for long-term implant performance. Medknow Publications & Media Pvt Ltd 2012 /pmc/articles/PMC3339246/ /pubmed/22736904 http://dx.doi.org/10.4103/0971-6580.94513 Text en Copyright: © Toxicology International http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Umashankar, P.R.
Mohanan, P.V.
Kumari, T.V.
Glutaraldehyde Treatment Elicits Toxic Response Compared to Decellularization in Bovine Pericardium
title Glutaraldehyde Treatment Elicits Toxic Response Compared to Decellularization in Bovine Pericardium
title_full Glutaraldehyde Treatment Elicits Toxic Response Compared to Decellularization in Bovine Pericardium
title_fullStr Glutaraldehyde Treatment Elicits Toxic Response Compared to Decellularization in Bovine Pericardium
title_full_unstemmed Glutaraldehyde Treatment Elicits Toxic Response Compared to Decellularization in Bovine Pericardium
title_short Glutaraldehyde Treatment Elicits Toxic Response Compared to Decellularization in Bovine Pericardium
title_sort glutaraldehyde treatment elicits toxic response compared to decellularization in bovine pericardium
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3339246/
https://www.ncbi.nlm.nih.gov/pubmed/22736904
http://dx.doi.org/10.4103/0971-6580.94513
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