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Ageing prolongs inflammatory marker expression in regenerating rat skeletal muscles after injury
BACKGROUND: Some of the most serious consequences of normal ageing relate to its effects on skeletal muscle, particularly significant wasting and associated weakness, termed "sarcopenia". The underlying mechanisms of sarcopenia have yet to be elucidated completely but an altered muscle inf...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3339359/ https://www.ncbi.nlm.nih.gov/pubmed/22206492 http://dx.doi.org/10.1186/1476-9255-8-41 |
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author | van der Poel, Chris Gosselin, Luc E Schertzer, Jonathan D Ryall, James G Swiderski, Kristy Wondemaghen, Meron Lynch, Gordon S |
author_facet | van der Poel, Chris Gosselin, Luc E Schertzer, Jonathan D Ryall, James G Swiderski, Kristy Wondemaghen, Meron Lynch, Gordon S |
author_sort | van der Poel, Chris |
collection | PubMed |
description | BACKGROUND: Some of the most serious consequences of normal ageing relate to its effects on skeletal muscle, particularly significant wasting and associated weakness, termed "sarcopenia". The underlying mechanisms of sarcopenia have yet to be elucidated completely but an altered muscle inflammatory response after injury is a likely contributing factor. In this study we investigated age-related changes in the expression of numerous inflammatory markers linked to successful muscle regeneration. METHODS: Right extensor digitorum longus (EDL) muscles from young (3 month), adult (12 month) and old (24 month) male F344 rats were injected with bupivacaine hydrochloride to cause complete muscle fibre degeneration, then excised 12, 24, 36, and 72 hours later (n = 5/age group/time point). We used qRT-PCR to quantify the mRNA expression levels of the inflammatory markers TNFα, IFNγ, IL1, IL18, IL6, and CD18 as well as regenerative markers MyoD and myogenin. RESULTS: Inflammatory markers were all increased significantly in all age groups after myotoxic injury. There was a trend for expression of inflammatory markers to be higher in uninjured muscles of old rats, especially at 72 hours post injury where the expression levels of several markers was significantly higher in old compared with young and adult rats. There was also a decrease in the expression of regenerative markers in old rats at 72 hours post injury. CONCLUSION: Our findings identify a prolonged inflammatory signature in injured muscles from old compared with young and adult rats together with a blunted expression of key markers of regeneration in muscles of old rats. Importantly, our findings identify potential targets for future therapeutic strategies for improving the regenerative capacity of skeletal muscle during ageing. |
format | Online Article Text |
id | pubmed-3339359 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-33393592012-05-01 Ageing prolongs inflammatory marker expression in regenerating rat skeletal muscles after injury van der Poel, Chris Gosselin, Luc E Schertzer, Jonathan D Ryall, James G Swiderski, Kristy Wondemaghen, Meron Lynch, Gordon S J Inflamm (Lond) Research BACKGROUND: Some of the most serious consequences of normal ageing relate to its effects on skeletal muscle, particularly significant wasting and associated weakness, termed "sarcopenia". The underlying mechanisms of sarcopenia have yet to be elucidated completely but an altered muscle inflammatory response after injury is a likely contributing factor. In this study we investigated age-related changes in the expression of numerous inflammatory markers linked to successful muscle regeneration. METHODS: Right extensor digitorum longus (EDL) muscles from young (3 month), adult (12 month) and old (24 month) male F344 rats were injected with bupivacaine hydrochloride to cause complete muscle fibre degeneration, then excised 12, 24, 36, and 72 hours later (n = 5/age group/time point). We used qRT-PCR to quantify the mRNA expression levels of the inflammatory markers TNFα, IFNγ, IL1, IL18, IL6, and CD18 as well as regenerative markers MyoD and myogenin. RESULTS: Inflammatory markers were all increased significantly in all age groups after myotoxic injury. There was a trend for expression of inflammatory markers to be higher in uninjured muscles of old rats, especially at 72 hours post injury where the expression levels of several markers was significantly higher in old compared with young and adult rats. There was also a decrease in the expression of regenerative markers in old rats at 72 hours post injury. CONCLUSION: Our findings identify a prolonged inflammatory signature in injured muscles from old compared with young and adult rats together with a blunted expression of key markers of regeneration in muscles of old rats. Importantly, our findings identify potential targets for future therapeutic strategies for improving the regenerative capacity of skeletal muscle during ageing. BioMed Central 2011-12-29 /pmc/articles/PMC3339359/ /pubmed/22206492 http://dx.doi.org/10.1186/1476-9255-8-41 Text en Copyright ©2011 van der Poel et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research van der Poel, Chris Gosselin, Luc E Schertzer, Jonathan D Ryall, James G Swiderski, Kristy Wondemaghen, Meron Lynch, Gordon S Ageing prolongs inflammatory marker expression in regenerating rat skeletal muscles after injury |
title | Ageing prolongs inflammatory marker expression in regenerating rat skeletal muscles after injury |
title_full | Ageing prolongs inflammatory marker expression in regenerating rat skeletal muscles after injury |
title_fullStr | Ageing prolongs inflammatory marker expression in regenerating rat skeletal muscles after injury |
title_full_unstemmed | Ageing prolongs inflammatory marker expression in regenerating rat skeletal muscles after injury |
title_short | Ageing prolongs inflammatory marker expression in regenerating rat skeletal muscles after injury |
title_sort | ageing prolongs inflammatory marker expression in regenerating rat skeletal muscles after injury |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3339359/ https://www.ncbi.nlm.nih.gov/pubmed/22206492 http://dx.doi.org/10.1186/1476-9255-8-41 |
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