New microsatellite markers for assessment of genetic diversity in date palm (Phoenix dactylifera L.)

New primer pairs of genomic DNA microsatellite markers were tested to assess the genetic diversity of eleven date palm genotypes. The results indicated that out of thirty, only seven primers (23.3%) failed to amplify the expected PCR fragments, while thirteen primers (43.3%) amplified monomorphic ba...

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Detalles Bibliográficos
Autores principales: Elmeer, Khaled, Sarwath, Hina, Malek, Joel, Baum, Michael, Hamwieh, Aladdin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2011
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3339606/
https://www.ncbi.nlm.nih.gov/pubmed/22582150
http://dx.doi.org/10.1007/s13205-011-0010-z
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author Elmeer, Khaled
Sarwath, Hina
Malek, Joel
Baum, Michael
Hamwieh, Aladdin
author_facet Elmeer, Khaled
Sarwath, Hina
Malek, Joel
Baum, Michael
Hamwieh, Aladdin
author_sort Elmeer, Khaled
collection PubMed
description New primer pairs of genomic DNA microsatellite markers were tested to assess the genetic diversity of eleven date palm genotypes. The results indicated that out of thirty, only seven primers (23.3%) failed to amplify the expected PCR fragments, while thirteen primers (43.3%) amplified monomorphic banding patterns and the remaining ten primers (33.4%) generated polymorphic banding patterns. A total of 77 alleles have been observed with a mean of 7.7 alleles per locus. The average of gene diversity was 0.80 ranging from 0.6 (in marker DP168) to 0.9 (in two markers DP157 and DP175). These new co-dominant markers will be a starting point for researchers making use of the markers for genetic mapping and diversity analysis of date palm.
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spelling pubmed-33396062012-05-09 New microsatellite markers for assessment of genetic diversity in date palm (Phoenix dactylifera L.) Elmeer, Khaled Sarwath, Hina Malek, Joel Baum, Michael Hamwieh, Aladdin 3 Biotech Original Article New primer pairs of genomic DNA microsatellite markers were tested to assess the genetic diversity of eleven date palm genotypes. The results indicated that out of thirty, only seven primers (23.3%) failed to amplify the expected PCR fragments, while thirteen primers (43.3%) amplified monomorphic banding patterns and the remaining ten primers (33.4%) generated polymorphic banding patterns. A total of 77 alleles have been observed with a mean of 7.7 alleles per locus. The average of gene diversity was 0.80 ranging from 0.6 (in marker DP168) to 0.9 (in two markers DP157 and DP175). These new co-dominant markers will be a starting point for researchers making use of the markers for genetic mapping and diversity analysis of date palm. Springer Berlin Heidelberg 2011-05-27 2011-09 /pmc/articles/PMC3339606/ /pubmed/22582150 http://dx.doi.org/10.1007/s13205-011-0010-z Text en © The Author(s) 2011 https://creativecommons.org/licenses/by/4.0/ This article is published under license to BioMed Central Ltd. Open Access This article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution and reproduction in any medium, provided the original author(s) and source are credited.
spellingShingle Original Article
Elmeer, Khaled
Sarwath, Hina
Malek, Joel
Baum, Michael
Hamwieh, Aladdin
New microsatellite markers for assessment of genetic diversity in date palm (Phoenix dactylifera L.)
title New microsatellite markers for assessment of genetic diversity in date palm (Phoenix dactylifera L.)
title_full New microsatellite markers for assessment of genetic diversity in date palm (Phoenix dactylifera L.)
title_fullStr New microsatellite markers for assessment of genetic diversity in date palm (Phoenix dactylifera L.)
title_full_unstemmed New microsatellite markers for assessment of genetic diversity in date palm (Phoenix dactylifera L.)
title_short New microsatellite markers for assessment of genetic diversity in date palm (Phoenix dactylifera L.)
title_sort new microsatellite markers for assessment of genetic diversity in date palm (phoenix dactylifera l.)
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3339606/
https://www.ncbi.nlm.nih.gov/pubmed/22582150
http://dx.doi.org/10.1007/s13205-011-0010-z
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