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Fluorescence Recovery After Photobleaching (FRAP) of Fluorescence Tagged Proteins in Dendritic Spines of Cultured Hippocampal Neurons

FRAP has been used to quantify the mobility of GFP-tagged proteins. Using a strong excitation laser, the fluorescence of a GFP-tagged protein is bleached in the region of interest. The fluorescence of the region recovers when the unbleached GFP-tagged protein from outside of the region diffuses into...

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Detalles Bibliográficos
Autores principales:	Zheng, Chan-Ying, Petralia, Ronald S., Wang, Ya-Xian, Kachar, Bechara
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	MyJove Corporation 2011
Materias:	Neuroscience
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3339873/ https://www.ncbi.nlm.nih.gov/pubmed/21525845 http://dx.doi.org/10.3791/2568

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