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Transcriptome Analysis in Tardigrade Species Reveals Specific Molecular Pathways for Stress Adaptations

Tardigrades have unique stress-adaptations that allow them to survive extremes of cold, heat, radiation and vacuum. To study this, encoded protein clusters and pathways from an ongoing transcriptome study on the tardigrade Milnesium tardigradum were analyzed using bioinformatics tools and compared t...

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Autores principales: Förster, Frank, Beisser, Daniela, Grohme, Markus A., Liang, Chunguang, Mali, Brahim, Siegl, Alexander Matthias, Engelmann, Julia C., Shkumatov, Alexander V., Schokraie, Elham, Müller, Tobias, Schnölzer, Martina, Schill, Ralph O., Frohme, Marcus, Dandekar, Thomas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Libertas Academica 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3342025/
https://www.ncbi.nlm.nih.gov/pubmed/22563243
http://dx.doi.org/10.4137/BBI.S9150
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author Förster, Frank
Beisser, Daniela
Grohme, Markus A.
Liang, Chunguang
Mali, Brahim
Siegl, Alexander Matthias
Engelmann, Julia C.
Shkumatov, Alexander V.
Schokraie, Elham
Müller, Tobias
Schnölzer, Martina
Schill, Ralph O.
Frohme, Marcus
Dandekar, Thomas
author_facet Förster, Frank
Beisser, Daniela
Grohme, Markus A.
Liang, Chunguang
Mali, Brahim
Siegl, Alexander Matthias
Engelmann, Julia C.
Shkumatov, Alexander V.
Schokraie, Elham
Müller, Tobias
Schnölzer, Martina
Schill, Ralph O.
Frohme, Marcus
Dandekar, Thomas
author_sort Förster, Frank
collection PubMed
description Tardigrades have unique stress-adaptations that allow them to survive extremes of cold, heat, radiation and vacuum. To study this, encoded protein clusters and pathways from an ongoing transcriptome study on the tardigrade Milnesium tardigradum were analyzed using bioinformatics tools and compared to expressed sequence tags (ESTs) from Hypsibius dujardini, revealing major pathways involved in resistance against extreme environmental conditions. ESTs are available on the Tardigrade Workbench along with software and databank updates. Our analysis reveals that RNA stability motifs for M. tardigradum are different from typical motifs known from higher animals. M. tardigradum and H. dujardini protein clusters and conserved domains imply metabolic storage pathways for glycogen, glycolipids and specific secondary metabolism as well as stress response pathways (including heat shock proteins, bmh2, and specific repair pathways). Redox-, DNA-, stress- and protein protection pathways complement specific repair capabilities to achieve the strong robustness of M. tardigradum. These pathways are partly conserved in other animals and their manipulation could boost stress adaptation even in human cells. However, the unique combination of resistance and repair pathways make tardigrades and M. tardigradum in particular so highly stress resistant.
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spelling pubmed-33420252012-05-04 Transcriptome Analysis in Tardigrade Species Reveals Specific Molecular Pathways for Stress Adaptations Förster, Frank Beisser, Daniela Grohme, Markus A. Liang, Chunguang Mali, Brahim Siegl, Alexander Matthias Engelmann, Julia C. Shkumatov, Alexander V. Schokraie, Elham Müller, Tobias Schnölzer, Martina Schill, Ralph O. Frohme, Marcus Dandekar, Thomas Bioinform Biol Insights Original Research Tardigrades have unique stress-adaptations that allow them to survive extremes of cold, heat, radiation and vacuum. To study this, encoded protein clusters and pathways from an ongoing transcriptome study on the tardigrade Milnesium tardigradum were analyzed using bioinformatics tools and compared to expressed sequence tags (ESTs) from Hypsibius dujardini, revealing major pathways involved in resistance against extreme environmental conditions. ESTs are available on the Tardigrade Workbench along with software and databank updates. Our analysis reveals that RNA stability motifs for M. tardigradum are different from typical motifs known from higher animals. M. tardigradum and H. dujardini protein clusters and conserved domains imply metabolic storage pathways for glycogen, glycolipids and specific secondary metabolism as well as stress response pathways (including heat shock proteins, bmh2, and specific repair pathways). Redox-, DNA-, stress- and protein protection pathways complement specific repair capabilities to achieve the strong robustness of M. tardigradum. These pathways are partly conserved in other animals and their manipulation could boost stress adaptation even in human cells. However, the unique combination of resistance and repair pathways make tardigrades and M. tardigradum in particular so highly stress resistant. Libertas Academica 2012-04-23 /pmc/articles/PMC3342025/ /pubmed/22563243 http://dx.doi.org/10.4137/BBI.S9150 Text en © the author(s), publisher and licensee Libertas Academica Ltd. This is an open access article. Unrestricted non-commercial use is permitted provided the original work is properly cited.
spellingShingle Original Research
Förster, Frank
Beisser, Daniela
Grohme, Markus A.
Liang, Chunguang
Mali, Brahim
Siegl, Alexander Matthias
Engelmann, Julia C.
Shkumatov, Alexander V.
Schokraie, Elham
Müller, Tobias
Schnölzer, Martina
Schill, Ralph O.
Frohme, Marcus
Dandekar, Thomas
Transcriptome Analysis in Tardigrade Species Reveals Specific Molecular Pathways for Stress Adaptations
title Transcriptome Analysis in Tardigrade Species Reveals Specific Molecular Pathways for Stress Adaptations
title_full Transcriptome Analysis in Tardigrade Species Reveals Specific Molecular Pathways for Stress Adaptations
title_fullStr Transcriptome Analysis in Tardigrade Species Reveals Specific Molecular Pathways for Stress Adaptations
title_full_unstemmed Transcriptome Analysis in Tardigrade Species Reveals Specific Molecular Pathways for Stress Adaptations
title_short Transcriptome Analysis in Tardigrade Species Reveals Specific Molecular Pathways for Stress Adaptations
title_sort transcriptome analysis in tardigrade species reveals specific molecular pathways for stress adaptations
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3342025/
https://www.ncbi.nlm.nih.gov/pubmed/22563243
http://dx.doi.org/10.4137/BBI.S9150
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