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Insights into T Cell Recognition of Antigen: Significance of Two-Dimensional Kinetic Parameters
The T cell receptor (TCR) interacts with peptide-major histocompatibility complex (pMHC) to enable T cell development and trigger adaptive immune responses. For this reason, TCR:pMHC interactions have been intensely studied for over two decades. However, the details of how various binding parameters...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Research Foundation
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3342060/ https://www.ncbi.nlm.nih.gov/pubmed/22566966 http://dx.doi.org/10.3389/fimmu.2012.00086 |
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author | Edwards, Lindsay J. Zarnitsyna, Veronika I. Hood, Jennifer D. Evavold, Brian D. Zhu, Cheng |
author_facet | Edwards, Lindsay J. Zarnitsyna, Veronika I. Hood, Jennifer D. Evavold, Brian D. Zhu, Cheng |
author_sort | Edwards, Lindsay J. |
collection | PubMed |
description | The T cell receptor (TCR) interacts with peptide-major histocompatibility complex (pMHC) to enable T cell development and trigger adaptive immune responses. For this reason, TCR:pMHC interactions have been intensely studied for over two decades. However, the details of how various binding parameters impact T cell activation remain elusive. Most measurements were made using recombinant proteins by surface plasmon resonance, a three-dimensional (3D) technique in which fluid-phase receptors and ligands are removed from their cellular environment. This approach found TCR:pMHC interactions with relatively low affinities and slow off-rates for agonist peptides. Newer generation techniques have analyzed TCR:pMHC interactions in two dimensions (2D), with both proteins anchored in apposing plasma membranes. These approaches reveal in situ TCR:pMHC interaction kinetics that are of high affinity and exhibit rapid on- and off-rates upon interaction with agonist ligands. Importantly, 2D binding parameters correlate better with T cell functional responses to a spectrum of ligands than 3D measures. |
format | Online Article Text |
id | pubmed-3342060 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Frontiers Research Foundation |
record_format | MEDLINE/PubMed |
spelling | pubmed-33420602012-05-07 Insights into T Cell Recognition of Antigen: Significance of Two-Dimensional Kinetic Parameters Edwards, Lindsay J. Zarnitsyna, Veronika I. Hood, Jennifer D. Evavold, Brian D. Zhu, Cheng Front Immunol Immunology The T cell receptor (TCR) interacts with peptide-major histocompatibility complex (pMHC) to enable T cell development and trigger adaptive immune responses. For this reason, TCR:pMHC interactions have been intensely studied for over two decades. However, the details of how various binding parameters impact T cell activation remain elusive. Most measurements were made using recombinant proteins by surface plasmon resonance, a three-dimensional (3D) technique in which fluid-phase receptors and ligands are removed from their cellular environment. This approach found TCR:pMHC interactions with relatively low affinities and slow off-rates for agonist peptides. Newer generation techniques have analyzed TCR:pMHC interactions in two dimensions (2D), with both proteins anchored in apposing plasma membranes. These approaches reveal in situ TCR:pMHC interaction kinetics that are of high affinity and exhibit rapid on- and off-rates upon interaction with agonist ligands. Importantly, 2D binding parameters correlate better with T cell functional responses to a spectrum of ligands than 3D measures. Frontiers Research Foundation 2012-04-20 /pmc/articles/PMC3342060/ /pubmed/22566966 http://dx.doi.org/10.3389/fimmu.2012.00086 Text en Copyright © 2012 Edwards, Zarnitsyna, Hood, Evavold and Zhu. http://www.frontiersin.org/licenseagreement This is an open-access article distributed under the terms of the Creative Commons Attribution Non Commercial License, which permits non-commercial use, distribution, and reproduction in other forums, provided the original authors and source are credited. |
spellingShingle | Immunology Edwards, Lindsay J. Zarnitsyna, Veronika I. Hood, Jennifer D. Evavold, Brian D. Zhu, Cheng Insights into T Cell Recognition of Antigen: Significance of Two-Dimensional Kinetic Parameters |
title | Insights into T Cell Recognition of Antigen: Significance of Two-Dimensional Kinetic Parameters |
title_full | Insights into T Cell Recognition of Antigen: Significance of Two-Dimensional Kinetic Parameters |
title_fullStr | Insights into T Cell Recognition of Antigen: Significance of Two-Dimensional Kinetic Parameters |
title_full_unstemmed | Insights into T Cell Recognition of Antigen: Significance of Two-Dimensional Kinetic Parameters |
title_short | Insights into T Cell Recognition of Antigen: Significance of Two-Dimensional Kinetic Parameters |
title_sort | insights into t cell recognition of antigen: significance of two-dimensional kinetic parameters |
topic | Immunology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3342060/ https://www.ncbi.nlm.nih.gov/pubmed/22566966 http://dx.doi.org/10.3389/fimmu.2012.00086 |
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