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Cell Surface Expression and Function of the Macromolecular C1 Complex on the Surface of Human Monocytes
The synthesis of the subunits of the C1 complex (C1q, C1s, C1r), and its regulator C1 inhibitor (C1-Inh) by human monocytes has been previously established. However, surface expression of these molecules by monocytes has not been shown. Using flow cytometry and antigen-capture enzyme-linked immunoso...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Research Foundation
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3342062/ https://www.ncbi.nlm.nih.gov/pubmed/22566921 http://dx.doi.org/10.3389/fimmu.2012.00038 |
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author | Hosszu, Kinga K. Valentino, Alisa Ji, Yan Matkovic, Mara Pednekar, Lina Rehage, Nina Tumma, Nithin Peerschke, Ellinor I. B. Ghebrehiwet, Berhane |
author_facet | Hosszu, Kinga K. Valentino, Alisa Ji, Yan Matkovic, Mara Pednekar, Lina Rehage, Nina Tumma, Nithin Peerschke, Ellinor I. B. Ghebrehiwet, Berhane |
author_sort | Hosszu, Kinga K. |
collection | PubMed |
description | The synthesis of the subunits of the C1 complex (C1q, C1s, C1r), and its regulator C1 inhibitor (C1-Inh) by human monocytes has been previously established. However, surface expression of these molecules by monocytes has not been shown. Using flow cytometry and antigen-capture enzyme-linked immunosorbent assay, we show here for the first time that, in addition to C1q, peripheral blood monocytes, and the monocyte-derived U937 cells express C1s and C1r, as well as Factor B and C1-Inh on their surface. C1s and C1r immunoprecipitated with C1q, suggesting that at least some of the C1q on these cells is part of the C1 complex. Furthermore, the C1 complex on U937 cells was able to trigger complement activation via the classical pathway. The presence of C1-Inh may ensure that an unwarranted autoactivation of the C1 complex does not take place. Since C1-Inh closely monitors the activation of the C1 complex in a sterile or infectious inflammatory environment, further elucidation of the role of C1 complex is crucial to dissect its function in monocyte, dendritic cell, and T cell activities, and its implications in host defense and tolerance. |
format | Online Article Text |
id | pubmed-3342062 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Frontiers Research Foundation |
record_format | MEDLINE/PubMed |
spelling | pubmed-33420622012-05-07 Cell Surface Expression and Function of the Macromolecular C1 Complex on the Surface of Human Monocytes Hosszu, Kinga K. Valentino, Alisa Ji, Yan Matkovic, Mara Pednekar, Lina Rehage, Nina Tumma, Nithin Peerschke, Ellinor I. B. Ghebrehiwet, Berhane Front Immunol Immunology The synthesis of the subunits of the C1 complex (C1q, C1s, C1r), and its regulator C1 inhibitor (C1-Inh) by human monocytes has been previously established. However, surface expression of these molecules by monocytes has not been shown. Using flow cytometry and antigen-capture enzyme-linked immunosorbent assay, we show here for the first time that, in addition to C1q, peripheral blood monocytes, and the monocyte-derived U937 cells express C1s and C1r, as well as Factor B and C1-Inh on their surface. C1s and C1r immunoprecipitated with C1q, suggesting that at least some of the C1q on these cells is part of the C1 complex. Furthermore, the C1 complex on U937 cells was able to trigger complement activation via the classical pathway. The presence of C1-Inh may ensure that an unwarranted autoactivation of the C1 complex does not take place. Since C1-Inh closely monitors the activation of the C1 complex in a sterile or infectious inflammatory environment, further elucidation of the role of C1 complex is crucial to dissect its function in monocyte, dendritic cell, and T cell activities, and its implications in host defense and tolerance. Frontiers Research Foundation 2012-03-05 /pmc/articles/PMC3342062/ /pubmed/22566921 http://dx.doi.org/10.3389/fimmu.2012.00038 Text en Copyright © 2012 Hosszu, Valentino, Ji, Matkovic, Pednekar, Rehage, Tumma, Peerschke and Ghebrehiwet. http://www.frontiersin.org/licenseagreement This is an open-access article distributed under the terms of the Creative Commons Attribution Non Commercial License, which permits non-commercial use, distribution, and reproduction in other forums, provided the original authors and source are credited. |
spellingShingle | Immunology Hosszu, Kinga K. Valentino, Alisa Ji, Yan Matkovic, Mara Pednekar, Lina Rehage, Nina Tumma, Nithin Peerschke, Ellinor I. B. Ghebrehiwet, Berhane Cell Surface Expression and Function of the Macromolecular C1 Complex on the Surface of Human Monocytes |
title | Cell Surface Expression and Function of the Macromolecular C1 Complex on the Surface of Human Monocytes |
title_full | Cell Surface Expression and Function of the Macromolecular C1 Complex on the Surface of Human Monocytes |
title_fullStr | Cell Surface Expression and Function of the Macromolecular C1 Complex on the Surface of Human Monocytes |
title_full_unstemmed | Cell Surface Expression and Function of the Macromolecular C1 Complex on the Surface of Human Monocytes |
title_short | Cell Surface Expression and Function of the Macromolecular C1 Complex on the Surface of Human Monocytes |
title_sort | cell surface expression and function of the macromolecular c1 complex on the surface of human monocytes |
topic | Immunology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3342062/ https://www.ncbi.nlm.nih.gov/pubmed/22566921 http://dx.doi.org/10.3389/fimmu.2012.00038 |
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