In vivo imaging of unstained tissues using long gradient index lens multiphoton endoscopic systems

We characterize long (up to 285 mm) gradient index (GRIN) lens endoscope systems for multiphoton imaging. We fabricate a portable, rigid endoscope system suitable for imaging unstained tissues, potentially deep within the body, using a GRIN lens system of 1 mm diameter and 8 cm length. The portable...

Descripción completa

Detalles Bibliográficos
Autores principales: Huland, David M., Brown, Christopher M., Howard, Scott S., Ouzounov, Dimitre G., Pavlova, Ina, Wang, Ke, Rivera, David R., Webb, Watt W., Xu, Chris
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Optical Society of America 2012
Materias:
Endoscopes, Catheters and Micro-Optics
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3342183/
https://www.ncbi.nlm.nih.gov/pubmed/22567597
http://dx.doi.org/10.1364/BOE.3.001077
Descripción
Sumario:We characterize long (up to 285 mm) gradient index (GRIN) lens endoscope systems for multiphoton imaging. We fabricate a portable, rigid endoscope system suitable for imaging unstained tissues, potentially deep within the body, using a GRIN lens system of 1 mm diameter and 8 cm length. The portable device is capable of imaging a ~200 µm diameter field of view at 4 frames/s. The lateral and axial resolution in water is 0.85 µm and 7.4 µm respectively. In vivo images of unstained tissues in live, anesthetized rats using the portable device are presented. These results show great promise for GRIN endoscopy to be used clinically.

Ejemplares similares