In vivo imaging of unstained tissues using long gradient index lens multiphoton endoscopic systems

We characterize long (up to 285 mm) gradient index (GRIN) lens endoscope systems for multiphoton imaging. We fabricate a portable, rigid endoscope system suitable for imaging unstained tissues, potentially deep within the body, using a GRIN lens system of 1 mm diameter and 8 cm length. The portable...

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Autores principales: Huland, David M., Brown, Christopher M., Howard, Scott S., Ouzounov, Dimitre G., Pavlova, Ina, Wang, Ke, Rivera, David R., Webb, Watt W., Xu, Chris
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Optical Society of America 2012
Materias:
Endoscopes, Catheters and Micro-Optics
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3342183/
https://www.ncbi.nlm.nih.gov/pubmed/22567597
http://dx.doi.org/10.1364/BOE.3.001077
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author Huland, David M.
Brown, Christopher M.
Howard, Scott S.
Ouzounov, Dimitre G.
Pavlova, Ina
Wang, Ke
Rivera, David R.
Webb, Watt W.
Xu, Chris
author_facet Huland, David M.
Brown, Christopher M.
Howard, Scott S.
Ouzounov, Dimitre G.
Pavlova, Ina
Wang, Ke
Rivera, David R.
Webb, Watt W.
Xu, Chris
author_sort Huland, David M.
collection PubMed
description We characterize long (up to 285 mm) gradient index (GRIN) lens endoscope systems for multiphoton imaging. We fabricate a portable, rigid endoscope system suitable for imaging unstained tissues, potentially deep within the body, using a GRIN lens system of 1 mm diameter and 8 cm length. The portable device is capable of imaging a ~200 µm diameter field of view at 4 frames/s. The lateral and axial resolution in water is 0.85 µm and 7.4 µm respectively. In vivo images of unstained tissues in live, anesthetized rats using the portable device are presented. These results show great promise for GRIN endoscopy to be used clinically.
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spelling pubmed-33421832012-05-07 In vivo imaging of unstained tissues using long gradient index lens multiphoton endoscopic systems Huland, David M. Brown, Christopher M. Howard, Scott S. Ouzounov, Dimitre G. Pavlova, Ina Wang, Ke Rivera, David R. Webb, Watt W. Xu, Chris Biomed Opt Express Endoscopes, Catheters and Micro-Optics We characterize long (up to 285 mm) gradient index (GRIN) lens endoscope systems for multiphoton imaging. We fabricate a portable, rigid endoscope system suitable for imaging unstained tissues, potentially deep within the body, using a GRIN lens system of 1 mm diameter and 8 cm length. The portable device is capable of imaging a ~200 µm diameter field of view at 4 frames/s. The lateral and axial resolution in water is 0.85 µm and 7.4 µm respectively. In vivo images of unstained tissues in live, anesthetized rats using the portable device are presented. These results show great promise for GRIN endoscopy to be used clinically. Optical Society of America 2012-04-19 /pmc/articles/PMC3342183/ /pubmed/22567597 http://dx.doi.org/10.1364/BOE.3.001077 Text en ©2012 Optical Society of America http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 Unported License, which permits download and redistribution, provided that the original work is properly cited. This license restricts the article from being modified or used commercially.
spellingShingle Endoscopes, Catheters and Micro-Optics
Huland, David M.
Brown, Christopher M.
Howard, Scott S.
Ouzounov, Dimitre G.
Pavlova, Ina
Wang, Ke
Rivera, David R.
Webb, Watt W.
Xu, Chris
In vivo imaging of unstained tissues using long gradient index lens multiphoton endoscopic systems
title In vivo imaging of unstained tissues using long gradient index lens multiphoton endoscopic systems
title_full In vivo imaging of unstained tissues using long gradient index lens multiphoton endoscopic systems
title_fullStr In vivo imaging of unstained tissues using long gradient index lens multiphoton endoscopic systems
title_full_unstemmed In vivo imaging of unstained tissues using long gradient index lens multiphoton endoscopic systems
title_short In vivo imaging of unstained tissues using long gradient index lens multiphoton endoscopic systems
title_sort in vivo imaging of unstained tissues using long gradient index lens multiphoton endoscopic systems
topic Endoscopes, Catheters and Micro-Optics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3342183/
https://www.ncbi.nlm.nih.gov/pubmed/22567597
http://dx.doi.org/10.1364/BOE.3.001077
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