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Enhanced localized plasmonic detections using partially-embedded gold nanoparticles and ellipsometric measurements
A cost-effective, stable and ultrasensitive localized surface plasmon resonance (LSPR) sensor based on gold nanoparticles (AuNPs) partially embedded in transparent substrate is presented. Partially embedded AuNPs were prepared by thermal annealing of gold thin films deposited on glass at a temperatu...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Optical Society of America
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3342195/ https://www.ncbi.nlm.nih.gov/pubmed/22567583 http://dx.doi.org/10.1364/BOE.3.000899 |
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author | Moirangthem, Rakesh Singh Yaseen, Mohammad Tariq Wei, Pei-Kuen Cheng, Ji-Yen Chang, Yia-Chung |
author_facet | Moirangthem, Rakesh Singh Yaseen, Mohammad Tariq Wei, Pei-Kuen Cheng, Ji-Yen Chang, Yia-Chung |
author_sort | Moirangthem, Rakesh Singh |
collection | PubMed |
description | A cost-effective, stable and ultrasensitive localized surface plasmon resonance (LSPR) sensor based on gold nanoparticles (AuNPs) partially embedded in transparent substrate is presented. Partially embedded AuNPs were prepared by thermal annealing of gold thin films deposited on glass at a temperature close to the glass transition temperature of the substrate. Annealed samples were optically characterized by using spectroscopic ellipsometry and compare with theoretical modeling to understand the optical responses from the samples. By combining the partially-embedded AuNPs substrate with a microfluidic flow cell and dove prism in an ellipsometry setup, an ultrasensitive change in the LSPR signal can be detected. The refractive index sensitivity obtained from the phase measurement is up to 1938 degrees/RIU which is several times higher than that of synthesized colloidal gold nanoparticles. The sample is further used to investigate the interactions between primary and secondary antibodies. The bio-molecular detection limit of the LSPR signal is down to 20 pM. Our proposed sensor is label free, non-destructive, with high sensitivity, low cost, and easy to fabricate. These features make it feasible for commercialization in biomedical applications. |
format | Online Article Text |
id | pubmed-3342195 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Optical Society of America |
record_format | MEDLINE/PubMed |
spelling | pubmed-33421952012-05-07 Enhanced localized plasmonic detections using partially-embedded gold nanoparticles and ellipsometric measurements Moirangthem, Rakesh Singh Yaseen, Mohammad Tariq Wei, Pei-Kuen Cheng, Ji-Yen Chang, Yia-Chung Biomed Opt Express Nanotechnology and Plasmonics A cost-effective, stable and ultrasensitive localized surface plasmon resonance (LSPR) sensor based on gold nanoparticles (AuNPs) partially embedded in transparent substrate is presented. Partially embedded AuNPs were prepared by thermal annealing of gold thin films deposited on glass at a temperature close to the glass transition temperature of the substrate. Annealed samples were optically characterized by using spectroscopic ellipsometry and compare with theoretical modeling to understand the optical responses from the samples. By combining the partially-embedded AuNPs substrate with a microfluidic flow cell and dove prism in an ellipsometry setup, an ultrasensitive change in the LSPR signal can be detected. The refractive index sensitivity obtained from the phase measurement is up to 1938 degrees/RIU which is several times higher than that of synthesized colloidal gold nanoparticles. The sample is further used to investigate the interactions between primary and secondary antibodies. The bio-molecular detection limit of the LSPR signal is down to 20 pM. Our proposed sensor is label free, non-destructive, with high sensitivity, low cost, and easy to fabricate. These features make it feasible for commercialization in biomedical applications. Optical Society of America 2012-04-09 /pmc/articles/PMC3342195/ /pubmed/22567583 http://dx.doi.org/10.1364/BOE.3.000899 Text en ©2012 Optical Society of America http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 Unported License, which permits download and redistribution, provided that the original work is properly cited. This license restricts the article from being modified or used commercially. |
spellingShingle | Nanotechnology and Plasmonics Moirangthem, Rakesh Singh Yaseen, Mohammad Tariq Wei, Pei-Kuen Cheng, Ji-Yen Chang, Yia-Chung Enhanced localized plasmonic detections using partially-embedded gold nanoparticles and ellipsometric measurements |
title | Enhanced localized plasmonic detections using partially-embedded gold nanoparticles and ellipsometric measurements |
title_full | Enhanced localized plasmonic detections using partially-embedded gold nanoparticles and ellipsometric measurements |
title_fullStr | Enhanced localized plasmonic detections using partially-embedded gold nanoparticles and ellipsometric measurements |
title_full_unstemmed | Enhanced localized plasmonic detections using partially-embedded gold nanoparticles and ellipsometric measurements |
title_short | Enhanced localized plasmonic detections using partially-embedded gold nanoparticles and ellipsometric measurements |
title_sort | enhanced localized plasmonic detections using partially-embedded gold nanoparticles and ellipsometric measurements |
topic | Nanotechnology and Plasmonics |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3342195/ https://www.ncbi.nlm.nih.gov/pubmed/22567583 http://dx.doi.org/10.1364/BOE.3.000899 |
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