Cargando…

Hepatic Ischemic Preconditioning Provides Protection Against Distant Renal Ischemia and Reperfusion Injury in Mice

We previously demonstrated that there are acute and delayed phases of renal protection against renal ischemia and reperfusion (IR) injury with renal ischemic preconditioning (IPC). This study assessed whether hepatic IPC could also reduce distant renal IR injury through the blood stream-mediated sup...

Descripción completa

Detalles Bibliográficos
Autores principales: Lee, Jung Ah, Choi, Jin Woo, In, Jang Hyeok, Jung, Hong Soo, Kim, Yong Shin, Jeon, Yeon Soo, Kang, Yoo Jin, Kim, Dae Woo, Lim, Yong Gul, Park, Jae Hee, Joo, Jin Deok
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Academy of Medical Sciences 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3342548/
https://www.ncbi.nlm.nih.gov/pubmed/22563222
http://dx.doi.org/10.3346/jkms.2012.27.5.547
_version_ 1782231708600369152
author Lee, Jung Ah
Choi, Jin Woo
In, Jang Hyeok
Jung, Hong Soo
Kim, Yong Shin
Jeon, Yeon Soo
Kang, Yoo Jin
Kim, Dae Woo
Lim, Yong Gul
Park, Jae Hee
Joo, Jin Deok
author_facet Lee, Jung Ah
Choi, Jin Woo
In, Jang Hyeok
Jung, Hong Soo
Kim, Yong Shin
Jeon, Yeon Soo
Kang, Yoo Jin
Kim, Dae Woo
Lim, Yong Gul
Park, Jae Hee
Joo, Jin Deok
author_sort Lee, Jung Ah
collection PubMed
description We previously demonstrated that there are acute and delayed phases of renal protection against renal ischemia and reperfusion (IR) injury with renal ischemic preconditioning (IPC). This study assessed whether hepatic IPC could also reduce distant renal IR injury through the blood stream-mediated supply of reactive oxygen species (ROS). Male C57BL/6 mice were randomly divided into four groups: group I, sham operated including right nephrectomy; group II (IR), left renal ischemia for 30 min and reperfusion injury; group III (IPC-IR), hepatic ischemia for 10 min followed by 10 min of reperfusion before left renal IR injury; group IV (MPG - IPC + IR), pretreated with 100 mg/kg N-(2-mercaptopropionyl)-glycine (MPG) 15 min before hepatic IPC and left renal IR injury. Renal function, histopathologic findings, proinflammatory cytokines, and cytoprotective proteins were evaluated 15 min or 24 hr after reperfusion. Hepatic IPC attenuated the expression of proinflammatory cytokines, tumor necrosis factor α, intercellular adhesion molecule 1, and induced inducible nitric-oxide synthase, and the phosphorylation of Akt in the murine kidney. Renal function was better preserved in mice with hepatic IPC (group III) than groups II or IV. Hepatic IPC protects against distant renal IR injury through the blood stream-delivery of hepatic IPC-induced ROS, by inducing cytoprotective proteins, and by inhibiting inflammatory reactions.
format Online
Article
Text
id pubmed-3342548
institution National Center for Biotechnology Information
language English
publishDate 2012
publisher The Korean Academy of Medical Sciences
record_format MEDLINE/PubMed
spelling pubmed-33425482012-05-04 Hepatic Ischemic Preconditioning Provides Protection Against Distant Renal Ischemia and Reperfusion Injury in Mice Lee, Jung Ah Choi, Jin Woo In, Jang Hyeok Jung, Hong Soo Kim, Yong Shin Jeon, Yeon Soo Kang, Yoo Jin Kim, Dae Woo Lim, Yong Gul Park, Jae Hee Joo, Jin Deok J Korean Med Sci Original Article We previously demonstrated that there are acute and delayed phases of renal protection against renal ischemia and reperfusion (IR) injury with renal ischemic preconditioning (IPC). This study assessed whether hepatic IPC could also reduce distant renal IR injury through the blood stream-mediated supply of reactive oxygen species (ROS). Male C57BL/6 mice were randomly divided into four groups: group I, sham operated including right nephrectomy; group II (IR), left renal ischemia for 30 min and reperfusion injury; group III (IPC-IR), hepatic ischemia for 10 min followed by 10 min of reperfusion before left renal IR injury; group IV (MPG - IPC + IR), pretreated with 100 mg/kg N-(2-mercaptopropionyl)-glycine (MPG) 15 min before hepatic IPC and left renal IR injury. Renal function, histopathologic findings, proinflammatory cytokines, and cytoprotective proteins were evaluated 15 min or 24 hr after reperfusion. Hepatic IPC attenuated the expression of proinflammatory cytokines, tumor necrosis factor α, intercellular adhesion molecule 1, and induced inducible nitric-oxide synthase, and the phosphorylation of Akt in the murine kidney. Renal function was better preserved in mice with hepatic IPC (group III) than groups II or IV. Hepatic IPC protects against distant renal IR injury through the blood stream-delivery of hepatic IPC-induced ROS, by inducing cytoprotective proteins, and by inhibiting inflammatory reactions. The Korean Academy of Medical Sciences 2012-05 2012-04-25 /pmc/articles/PMC3342548/ /pubmed/22563222 http://dx.doi.org/10.3346/jkms.2012.27.5.547 Text en © 2012 The Korean Academy of Medical Sciences. http://creativecommons.org/licenses/by-nc/3.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Lee, Jung Ah
Choi, Jin Woo
In, Jang Hyeok
Jung, Hong Soo
Kim, Yong Shin
Jeon, Yeon Soo
Kang, Yoo Jin
Kim, Dae Woo
Lim, Yong Gul
Park, Jae Hee
Joo, Jin Deok
Hepatic Ischemic Preconditioning Provides Protection Against Distant Renal Ischemia and Reperfusion Injury in Mice
title Hepatic Ischemic Preconditioning Provides Protection Against Distant Renal Ischemia and Reperfusion Injury in Mice
title_full Hepatic Ischemic Preconditioning Provides Protection Against Distant Renal Ischemia and Reperfusion Injury in Mice
title_fullStr Hepatic Ischemic Preconditioning Provides Protection Against Distant Renal Ischemia and Reperfusion Injury in Mice
title_full_unstemmed Hepatic Ischemic Preconditioning Provides Protection Against Distant Renal Ischemia and Reperfusion Injury in Mice
title_short Hepatic Ischemic Preconditioning Provides Protection Against Distant Renal Ischemia and Reperfusion Injury in Mice
title_sort hepatic ischemic preconditioning provides protection against distant renal ischemia and reperfusion injury in mice
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3342548/
https://www.ncbi.nlm.nih.gov/pubmed/22563222
http://dx.doi.org/10.3346/jkms.2012.27.5.547
work_keys_str_mv AT leejungah hepaticischemicpreconditioningprovidesprotectionagainstdistantrenalischemiaandreperfusioninjuryinmice
AT choijinwoo hepaticischemicpreconditioningprovidesprotectionagainstdistantrenalischemiaandreperfusioninjuryinmice
AT injanghyeok hepaticischemicpreconditioningprovidesprotectionagainstdistantrenalischemiaandreperfusioninjuryinmice
AT junghongsoo hepaticischemicpreconditioningprovidesprotectionagainstdistantrenalischemiaandreperfusioninjuryinmice
AT kimyongshin hepaticischemicpreconditioningprovidesprotectionagainstdistantrenalischemiaandreperfusioninjuryinmice
AT jeonyeonsoo hepaticischemicpreconditioningprovidesprotectionagainstdistantrenalischemiaandreperfusioninjuryinmice
AT kangyoojin hepaticischemicpreconditioningprovidesprotectionagainstdistantrenalischemiaandreperfusioninjuryinmice
AT kimdaewoo hepaticischemicpreconditioningprovidesprotectionagainstdistantrenalischemiaandreperfusioninjuryinmice
AT limyonggul hepaticischemicpreconditioningprovidesprotectionagainstdistantrenalischemiaandreperfusioninjuryinmice
AT parkjaehee hepaticischemicpreconditioningprovidesprotectionagainstdistantrenalischemiaandreperfusioninjuryinmice
AT joojindeok hepaticischemicpreconditioningprovidesprotectionagainstdistantrenalischemiaandreperfusioninjuryinmice