Development of 16 Microsatellite Markers for Prince’s Pine, Chimaphila japonica (Pyroleae, Monotropoideae, Ericaceae)

The perennial evergreen herb, Chimaphila japonica is found exclusively in East Asian temperate coniferous or sometimes in deciduous forests. By using the Fast Isolation by Amplified Fragment Length Polymorphism (AFLP) of Sequences Containing repeats (FIASCO) protocol, 20 microsatellite primer sets w...

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Detalles Bibliográficos
Autores principales: Liu, Zhenwen, Zhao, Qianru, Zhou, Jing, Peng, Hua, Yang, Junbo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2012
Materias:
Short Note
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3344197/
https://www.ncbi.nlm.nih.gov/pubmed/22605961
http://dx.doi.org/10.3390/ijms13044003
Descripción
Sumario:The perennial evergreen herb, Chimaphila japonica is found exclusively in East Asian temperate coniferous or sometimes in deciduous forests. By using the Fast Isolation by Amplified Fragment Length Polymorphism (AFLP) of Sequences Containing repeats (FIASCO) protocol, 20 microsatellite primer sets were identified in two wild populations. Of these primers, 16 displayed polymorphisms and 4 were monomorphic. The number of alleles per locus ranged from one to six among populations, values for expected and observed heterozygosities ranged from 0.000 to 0.848 and from 0.000 to 1.000, respectively. The new SSR markers will be useful in obtaining estimates of population-level genetic diversity and in phylogeographic studies of C. japonica.

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