Development of 16 Microsatellite Markers for Prince’s Pine, Chimaphila japonica (Pyroleae, Monotropoideae, Ericaceae)

The perennial evergreen herb, Chimaphila japonica is found exclusively in East Asian temperate coniferous or sometimes in deciduous forests. By using the Fast Isolation by Amplified Fragment Length Polymorphism (AFLP) of Sequences Containing repeats (FIASCO) protocol, 20 microsatellite primer sets w...

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Detalles Bibliográficos
Autores principales: Liu, Zhenwen, Zhao, Qianru, Zhou, Jing, Peng, Hua, Yang, Junbo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2012
Materias:
Short Note
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3344197/
https://www.ncbi.nlm.nih.gov/pubmed/22605961
http://dx.doi.org/10.3390/ijms13044003
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author Liu, Zhenwen
Zhao, Qianru
Zhou, Jing
Peng, Hua
Yang, Junbo
author_facet Liu, Zhenwen
Zhao, Qianru
Zhou, Jing
Peng, Hua
Yang, Junbo
author_sort Liu, Zhenwen
collection PubMed
description The perennial evergreen herb, Chimaphila japonica is found exclusively in East Asian temperate coniferous or sometimes in deciduous forests. By using the Fast Isolation by Amplified Fragment Length Polymorphism (AFLP) of Sequences Containing repeats (FIASCO) protocol, 20 microsatellite primer sets were identified in two wild populations. Of these primers, 16 displayed polymorphisms and 4 were monomorphic. The number of alleles per locus ranged from one to six among populations, values for expected and observed heterozygosities ranged from 0.000 to 0.848 and from 0.000 to 1.000, respectively. The new SSR markers will be useful in obtaining estimates of population-level genetic diversity and in phylogeographic studies of C. japonica.
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spelling pubmed-33441972012-05-17 Development of 16 Microsatellite Markers for Prince’s Pine, Chimaphila japonica (Pyroleae, Monotropoideae, Ericaceae) Liu, Zhenwen Zhao, Qianru Zhou, Jing Peng, Hua Yang, Junbo Int J Mol Sci Short Note The perennial evergreen herb, Chimaphila japonica is found exclusively in East Asian temperate coniferous or sometimes in deciduous forests. By using the Fast Isolation by Amplified Fragment Length Polymorphism (AFLP) of Sequences Containing repeats (FIASCO) protocol, 20 microsatellite primer sets were identified in two wild populations. Of these primers, 16 displayed polymorphisms and 4 were monomorphic. The number of alleles per locus ranged from one to six among populations, values for expected and observed heterozygosities ranged from 0.000 to 0.848 and from 0.000 to 1.000, respectively. The new SSR markers will be useful in obtaining estimates of population-level genetic diversity and in phylogeographic studies of C. japonica. Molecular Diversity Preservation International (MDPI) 2012-03-23 /pmc/articles/PMC3344197/ /pubmed/22605961 http://dx.doi.org/10.3390/ijms13044003 Text en © 2012 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0 This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Short Note
Liu, Zhenwen
Zhao, Qianru
Zhou, Jing
Peng, Hua
Yang, Junbo
Development of 16 Microsatellite Markers for Prince’s Pine, Chimaphila japonica (Pyroleae, Monotropoideae, Ericaceae)
title Development of 16 Microsatellite Markers for Prince’s Pine, Chimaphila japonica (Pyroleae, Monotropoideae, Ericaceae)
title_full Development of 16 Microsatellite Markers for Prince’s Pine, Chimaphila japonica (Pyroleae, Monotropoideae, Ericaceae)
title_fullStr Development of 16 Microsatellite Markers for Prince’s Pine, Chimaphila japonica (Pyroleae, Monotropoideae, Ericaceae)
title_full_unstemmed Development of 16 Microsatellite Markers for Prince’s Pine, Chimaphila japonica (Pyroleae, Monotropoideae, Ericaceae)
title_short Development of 16 Microsatellite Markers for Prince’s Pine, Chimaphila japonica (Pyroleae, Monotropoideae, Ericaceae)
title_sort development of 16 microsatellite markers for prince’s pine, chimaphila japonica (pyroleae, monotropoideae, ericaceae)
topic Short Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3344197/
https://www.ncbi.nlm.nih.gov/pubmed/22605961
http://dx.doi.org/10.3390/ijms13044003
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