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Trypanosoma cruzi Immune Response Modulation Decreases Microbiota in Rhodnius prolixus Gut and Is Crucial for Parasite Survival and Development

Trypanosoma cruzi in order to complete its development in the digestive tract of Rhodnius prolixus needs to overcome the immune reactions and microbiota trypanolytic activity of the gut. We demonstrate that in R. prolixus following infection with epimastigotes of Trypanosoma cruzi clone Dm28c and, i...

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Autores principales: Castro, Daniele P., Moraes, Caroline S., Gonzalez, Marcelo S., Ratcliffe, Norman A., Azambuja, Patrícia, Garcia, Eloi S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3344921/
https://www.ncbi.nlm.nih.gov/pubmed/22574189
http://dx.doi.org/10.1371/journal.pone.0036591
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author Castro, Daniele P.
Moraes, Caroline S.
Gonzalez, Marcelo S.
Ratcliffe, Norman A.
Azambuja, Patrícia
Garcia, Eloi S.
author_facet Castro, Daniele P.
Moraes, Caroline S.
Gonzalez, Marcelo S.
Ratcliffe, Norman A.
Azambuja, Patrícia
Garcia, Eloi S.
author_sort Castro, Daniele P.
collection PubMed
description Trypanosoma cruzi in order to complete its development in the digestive tract of Rhodnius prolixus needs to overcome the immune reactions and microbiota trypanolytic activity of the gut. We demonstrate that in R. prolixus following infection with epimastigotes of Trypanosoma cruzi clone Dm28c and, in comparison with uninfected control insects, the midgut contained (i) fewer bacteria, (ii) higher parasite numbers, and (iii) reduced nitrite and nitrate production and increased phenoloxidase and antibacterial activities. In addition, in insects pre-treated with antibiotic and then infected with Dm28c, there were also reduced bacteria numbers and a higher parasite load compared with insects solely infected with parasites. Furthermore, and in contrast to insects infected with Dm28c, infection with T. cruzi Y strain resulted in a slight decreased numbers of gut bacteria but not sufficient to mediate a successful parasite infection. We conclude that infection of R. prolixus with the T. cruzi Dm28c clone modifies the host gut immune responses to decrease the microbiota population and these changes are crucial for the parasite development in the insect gut.
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spelling pubmed-33449212012-05-09 Trypanosoma cruzi Immune Response Modulation Decreases Microbiota in Rhodnius prolixus Gut and Is Crucial for Parasite Survival and Development Castro, Daniele P. Moraes, Caroline S. Gonzalez, Marcelo S. Ratcliffe, Norman A. Azambuja, Patrícia Garcia, Eloi S. PLoS One Research Article Trypanosoma cruzi in order to complete its development in the digestive tract of Rhodnius prolixus needs to overcome the immune reactions and microbiota trypanolytic activity of the gut. We demonstrate that in R. prolixus following infection with epimastigotes of Trypanosoma cruzi clone Dm28c and, in comparison with uninfected control insects, the midgut contained (i) fewer bacteria, (ii) higher parasite numbers, and (iii) reduced nitrite and nitrate production and increased phenoloxidase and antibacterial activities. In addition, in insects pre-treated with antibiotic and then infected with Dm28c, there were also reduced bacteria numbers and a higher parasite load compared with insects solely infected with parasites. Furthermore, and in contrast to insects infected with Dm28c, infection with T. cruzi Y strain resulted in a slight decreased numbers of gut bacteria but not sufficient to mediate a successful parasite infection. We conclude that infection of R. prolixus with the T. cruzi Dm28c clone modifies the host gut immune responses to decrease the microbiota population and these changes are crucial for the parasite development in the insect gut. Public Library of Science 2012-05-04 /pmc/articles/PMC3344921/ /pubmed/22574189 http://dx.doi.org/10.1371/journal.pone.0036591 Text en Castro et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Castro, Daniele P.
Moraes, Caroline S.
Gonzalez, Marcelo S.
Ratcliffe, Norman A.
Azambuja, Patrícia
Garcia, Eloi S.
Trypanosoma cruzi Immune Response Modulation Decreases Microbiota in Rhodnius prolixus Gut and Is Crucial for Parasite Survival and Development
title Trypanosoma cruzi Immune Response Modulation Decreases Microbiota in Rhodnius prolixus Gut and Is Crucial for Parasite Survival and Development
title_full Trypanosoma cruzi Immune Response Modulation Decreases Microbiota in Rhodnius prolixus Gut and Is Crucial for Parasite Survival and Development
title_fullStr Trypanosoma cruzi Immune Response Modulation Decreases Microbiota in Rhodnius prolixus Gut and Is Crucial for Parasite Survival and Development
title_full_unstemmed Trypanosoma cruzi Immune Response Modulation Decreases Microbiota in Rhodnius prolixus Gut and Is Crucial for Parasite Survival and Development
title_short Trypanosoma cruzi Immune Response Modulation Decreases Microbiota in Rhodnius prolixus Gut and Is Crucial for Parasite Survival and Development
title_sort trypanosoma cruzi immune response modulation decreases microbiota in rhodnius prolixus gut and is crucial for parasite survival and development
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3344921/
https://www.ncbi.nlm.nih.gov/pubmed/22574189
http://dx.doi.org/10.1371/journal.pone.0036591
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