Development of a Rapid and Confirmatory Method to Identify Ganoderic Acids in Ganoderma Mushrooms

To examine the composition of lanostanoids in Ganoderma lucidum, we have developed a liquid chromatography–mass spectrometry (LC–MS) method by using the ganoderic acids isolated in our laboratory as reference standards. The identity of 14 peaks in the high performance liquid chromatogram (HPLC) of G...

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Autores principales: Qi, Ying, Zhao, Lingling, Sun, Hao H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Research Foundation 2012
Materias:
Pharmacology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3345583/
https://www.ncbi.nlm.nih.gov/pubmed/22586399
http://dx.doi.org/10.3389/fphar.2012.00085
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author Qi, Ying
Zhao, Lingling
Sun, Hao H.
author_facet Qi, Ying
Zhao, Lingling
Sun, Hao H.
author_sort Qi, Ying
collection PubMed
description To examine the composition of lanostanoids in Ganoderma lucidum, we have developed a liquid chromatography–mass spectrometry (LC–MS) method by using the ganoderic acids isolated in our laboratory as reference standards. The identity of 14 peaks in the high performance liquid chromatogram (HPLC) of G. lucidum has been confirmed. By using the HPLC retention times of these ganoderic acids and their mass fragmentation patterns established in this paper, one can use LC–MS to analyze G. lucidum without requiring the reference standards of these 14 ganoderic acids. Subsequently, only the HPLC–UV method would be needed to analyze routine samples of G. lucidum.
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spelling pubmed-33455832012-05-14 Development of a Rapid and Confirmatory Method to Identify Ganoderic Acids in Ganoderma Mushrooms Qi, Ying Zhao, Lingling Sun, Hao H. Front Pharmacol Pharmacology To examine the composition of lanostanoids in Ganoderma lucidum, we have developed a liquid chromatography–mass spectrometry (LC–MS) method by using the ganoderic acids isolated in our laboratory as reference standards. The identity of 14 peaks in the high performance liquid chromatogram (HPLC) of G. lucidum has been confirmed. By using the HPLC retention times of these ganoderic acids and their mass fragmentation patterns established in this paper, one can use LC–MS to analyze G. lucidum without requiring the reference standards of these 14 ganoderic acids. Subsequently, only the HPLC–UV method would be needed to analyze routine samples of G. lucidum. Frontiers Research Foundation 2012-05-07 /pmc/articles/PMC3345583/ /pubmed/22586399 http://dx.doi.org/10.3389/fphar.2012.00085 Text en Copyright © 2012 Qi, Zhao and Sun. http://www.frontiersin.org/licenseagreement This is an open-access article distributed under the terms of the Creative Commons Attribution Non Commercial License, which permits non-commercial use, distribution, and reproduction in other forums, provided the original authors and source are credited.
spellingShingle Pharmacology
Qi, Ying
Zhao, Lingling
Sun, Hao H.
Development of a Rapid and Confirmatory Method to Identify Ganoderic Acids in Ganoderma Mushrooms
title Development of a Rapid and Confirmatory Method to Identify Ganoderic Acids in Ganoderma Mushrooms
title_full Development of a Rapid and Confirmatory Method to Identify Ganoderic Acids in Ganoderma Mushrooms
title_fullStr Development of a Rapid and Confirmatory Method to Identify Ganoderic Acids in Ganoderma Mushrooms
title_full_unstemmed Development of a Rapid and Confirmatory Method to Identify Ganoderic Acids in Ganoderma Mushrooms
title_short Development of a Rapid and Confirmatory Method to Identify Ganoderic Acids in Ganoderma Mushrooms
title_sort development of a rapid and confirmatory method to identify ganoderic acids in ganoderma mushrooms
topic Pharmacology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3345583/
https://www.ncbi.nlm.nih.gov/pubmed/22586399
http://dx.doi.org/10.3389/fphar.2012.00085
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