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Automation of a primer design and evaluation pipeline for subsequent sequencing of the coding regions of all human Refseq genes

Screening for mutations in human disease-causing genes in a molecular diagnostic environment demands simplicity with a view to allowing high throughput approaches. In order to advance these requirements, we have developed and applied a primer design program, termed BatchPD, to achieve the PCR amplif...

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Detalles Bibliográficos
Autores principales: Lai, Daniel, Love, Donald R
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Biomedical Informatics 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3346021/
https://www.ncbi.nlm.nih.gov/pubmed/22570517
http://dx.doi.org/10.6026/97320630008365