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Automation of a primer design and evaluation pipeline for subsequent sequencing of the coding regions of all human Refseq genes
Screening for mutations in human disease-causing genes in a molecular diagnostic environment demands simplicity with a view to allowing high throughput approaches. In order to advance these requirements, we have developed and applied a primer design program, termed BatchPD, to achieve the PCR amplif...
Autores principales: | Lai, Daniel, Love, Donald R |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Biomedical Informatics
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3346021/ https://www.ncbi.nlm.nih.gov/pubmed/22570517 http://dx.doi.org/10.6026/97320630008365 |
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