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Physiological and proteomic approaches to address the active role of ozone in kiwifruit post-harvest ripening

Post-harvest ozone application has recently been shown to inhibit the onset of senescence symptoms on fleshy fruit and vegetables; however, the exact mechanism of action is yet unknown. To characterize the impact of ozone on the post-harvest performance of kiwifruit (Actinidia deliciosa cv. ‘Hayward...

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Autores principales: Minas, Ioannis S., Tanou, Georgia, Belghazi, Maya, Job, Dominique, Manganaris, George A., Molassiotis, Athanassios, Vasilakakis, Miltiadis
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3346216/
https://www.ncbi.nlm.nih.gov/pubmed/22268155
http://dx.doi.org/10.1093/jxb/err418
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author Minas, Ioannis S.
Tanou, Georgia
Belghazi, Maya
Job, Dominique
Manganaris, George A.
Molassiotis, Athanassios
Vasilakakis, Miltiadis
author_facet Minas, Ioannis S.
Tanou, Georgia
Belghazi, Maya
Job, Dominique
Manganaris, George A.
Molassiotis, Athanassios
Vasilakakis, Miltiadis
author_sort Minas, Ioannis S.
collection PubMed
description Post-harvest ozone application has recently been shown to inhibit the onset of senescence symptoms on fleshy fruit and vegetables; however, the exact mechanism of action is yet unknown. To characterize the impact of ozone on the post-harvest performance of kiwifruit (Actinidia deliciosa cv. ‘Hayward’), fruits were cold stored (0 °C, 95% relative humidity) in a commercial ethylene-free room for 1, 3, or 5 months in the absence (control) or presence of ozone (0.3 μl l(−1)) and subsequently were allowed to ripen at a higher temperature (20 °C), herein defined as the shelf-life period, for up to 12 days. Ozone blocked ethylene production, delayed ripening, and stimulated antioxidant and anti-radical activities of fruits. Proteomic analysis using 1D-SDS-PAGE and mass spectrometry identified 102 kiwifruit proteins during ripening, which are mainly involved in energy, protein metabolism, defence, and cell structure. Ripening induced protein carbonylation in kiwifruit but this effect was depressed by ozone. A set of candidate kiwifruit proteins that are sensitive to carbonylation was also discovered. Overall, the present data indicate that ozone improved kiwifruit post-harvest behaviour, thus providing a first step towards understanding the active role of this molecule in fruit ripening.
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spelling pubmed-33462162012-05-07 Physiological and proteomic approaches to address the active role of ozone in kiwifruit post-harvest ripening Minas, Ioannis S. Tanou, Georgia Belghazi, Maya Job, Dominique Manganaris, George A. Molassiotis, Athanassios Vasilakakis, Miltiadis J Exp Bot Research Papers Post-harvest ozone application has recently been shown to inhibit the onset of senescence symptoms on fleshy fruit and vegetables; however, the exact mechanism of action is yet unknown. To characterize the impact of ozone on the post-harvest performance of kiwifruit (Actinidia deliciosa cv. ‘Hayward’), fruits were cold stored (0 °C, 95% relative humidity) in a commercial ethylene-free room for 1, 3, or 5 months in the absence (control) or presence of ozone (0.3 μl l(−1)) and subsequently were allowed to ripen at a higher temperature (20 °C), herein defined as the shelf-life period, for up to 12 days. Ozone blocked ethylene production, delayed ripening, and stimulated antioxidant and anti-radical activities of fruits. Proteomic analysis using 1D-SDS-PAGE and mass spectrometry identified 102 kiwifruit proteins during ripening, which are mainly involved in energy, protein metabolism, defence, and cell structure. Ripening induced protein carbonylation in kiwifruit but this effect was depressed by ozone. A set of candidate kiwifruit proteins that are sensitive to carbonylation was also discovered. Overall, the present data indicate that ozone improved kiwifruit post-harvest behaviour, thus providing a first step towards understanding the active role of this molecule in fruit ripening. Oxford University Press 2012-04 2012-01-20 /pmc/articles/PMC3346216/ /pubmed/22268155 http://dx.doi.org/10.1093/jxb/err418 Text en © 2012 The Author(s). http://creativecommons.org/licenses/by-nc/3.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. This paper is available online free of all access charges (see http://jxb.oxfordjournals.org/open_access.html for further details)
spellingShingle Research Papers
Minas, Ioannis S.
Tanou, Georgia
Belghazi, Maya
Job, Dominique
Manganaris, George A.
Molassiotis, Athanassios
Vasilakakis, Miltiadis
Physiological and proteomic approaches to address the active role of ozone in kiwifruit post-harvest ripening
title Physiological and proteomic approaches to address the active role of ozone in kiwifruit post-harvest ripening
title_full Physiological and proteomic approaches to address the active role of ozone in kiwifruit post-harvest ripening
title_fullStr Physiological and proteomic approaches to address the active role of ozone in kiwifruit post-harvest ripening
title_full_unstemmed Physiological and proteomic approaches to address the active role of ozone in kiwifruit post-harvest ripening
title_short Physiological and proteomic approaches to address the active role of ozone in kiwifruit post-harvest ripening
title_sort physiological and proteomic approaches to address the active role of ozone in kiwifruit post-harvest ripening
topic Research Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3346216/
https://www.ncbi.nlm.nih.gov/pubmed/22268155
http://dx.doi.org/10.1093/jxb/err418
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