Cargando…

Genetic Dissection of Sympatric Populations of Brown Planthopper, Nilaparvata lugens (Stål), Using DALP-PCR Molecular Markers

Direct amplified length polymorphism (DALP) combines the advantages of a high-resolution fingerprint method and also characterizing the genetic polymorphisms. This molecular method was also found to be useful in brown planthopper, Nilaparvata lugens species complex for the analysis of genetic polymo...

Descripción completa

Detalles Bibliográficos
Autores principales: Latif, M. A., Rafii, M. Y., Mazid, M. S., Ali, M. E., Ahmed, F., Omar, M. Y., Tan, S. G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Scientific World Journal 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3346741/
https://www.ncbi.nlm.nih.gov/pubmed/22593700
http://dx.doi.org/10.1100/2012/586831
_version_ 1782232218567966720
author Latif, M. A.
Rafii, M. Y.
Mazid, M. S.
Ali, M. E.
Ahmed, F.
Omar, M. Y.
Tan, S. G.
author_facet Latif, M. A.
Rafii, M. Y.
Mazid, M. S.
Ali, M. E.
Ahmed, F.
Omar, M. Y.
Tan, S. G.
author_sort Latif, M. A.
collection PubMed
description Direct amplified length polymorphism (DALP) combines the advantages of a high-resolution fingerprint method and also characterizing the genetic polymorphisms. This molecular method was also found to be useful in brown planthopper, Nilaparvata lugens species complex for the analysis of genetic polymorphisms. A total of 11 populations of Nilaparvata spp. were collected from 6 locations from Malaysia. Two sympatric populations of brown planthopper, N. lugens, one from rice and the other from a weed grass (Leersia hexandra), were collected from each of five locations. N. bakeri was used as an out group. Three oligonucleotide primer pairs, DALP231/DALPR′5, DALP234/DALPR′5, and DALP235/DALPR′5 were applied in this study. The unweighted pair group method with arithmetic mean (UPGMA) dendrogram based on genetic distances for the 11 populations of Nilaparvata spp. revealed that populations belonging to the same species and the same host type clustered together irrespective of their geographical localities of capture. The populations of N. lugens formed into two distinct clusters, one was insects with high esterase activities usually captured from rice and the other was with low esterase activities usually captured from L. hexandra. N. bakeri, an out group, was the most isolated group. Analyses of principal components, molecular variance, and robustness also supported greatly to the findings of cluster analysis.
format Online
Article
Text
id pubmed-3346741
institution National Center for Biotechnology Information
language English
publishDate 2012
publisher The Scientific World Journal
record_format MEDLINE/PubMed
spelling pubmed-33467412012-05-16 Genetic Dissection of Sympatric Populations of Brown Planthopper, Nilaparvata lugens (Stål), Using DALP-PCR Molecular Markers Latif, M. A. Rafii, M. Y. Mazid, M. S. Ali, M. E. Ahmed, F. Omar, M. Y. Tan, S. G. ScientificWorldJournal Research Article Direct amplified length polymorphism (DALP) combines the advantages of a high-resolution fingerprint method and also characterizing the genetic polymorphisms. This molecular method was also found to be useful in brown planthopper, Nilaparvata lugens species complex for the analysis of genetic polymorphisms. A total of 11 populations of Nilaparvata spp. were collected from 6 locations from Malaysia. Two sympatric populations of brown planthopper, N. lugens, one from rice and the other from a weed grass (Leersia hexandra), were collected from each of five locations. N. bakeri was used as an out group. Three oligonucleotide primer pairs, DALP231/DALPR′5, DALP234/DALPR′5, and DALP235/DALPR′5 were applied in this study. The unweighted pair group method with arithmetic mean (UPGMA) dendrogram based on genetic distances for the 11 populations of Nilaparvata spp. revealed that populations belonging to the same species and the same host type clustered together irrespective of their geographical localities of capture. The populations of N. lugens formed into two distinct clusters, one was insects with high esterase activities usually captured from rice and the other was with low esterase activities usually captured from L. hexandra. N. bakeri, an out group, was the most isolated group. Analyses of principal components, molecular variance, and robustness also supported greatly to the findings of cluster analysis. The Scientific World Journal 2012-04-19 /pmc/articles/PMC3346741/ /pubmed/22593700 http://dx.doi.org/10.1100/2012/586831 Text en Copyright © 2012 M. A. Latif et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Latif, M. A.
Rafii, M. Y.
Mazid, M. S.
Ali, M. E.
Ahmed, F.
Omar, M. Y.
Tan, S. G.
Genetic Dissection of Sympatric Populations of Brown Planthopper, Nilaparvata lugens (Stål), Using DALP-PCR Molecular Markers
title Genetic Dissection of Sympatric Populations of Brown Planthopper, Nilaparvata lugens (Stål), Using DALP-PCR Molecular Markers
title_full Genetic Dissection of Sympatric Populations of Brown Planthopper, Nilaparvata lugens (Stål), Using DALP-PCR Molecular Markers
title_fullStr Genetic Dissection of Sympatric Populations of Brown Planthopper, Nilaparvata lugens (Stål), Using DALP-PCR Molecular Markers
title_full_unstemmed Genetic Dissection of Sympatric Populations of Brown Planthopper, Nilaparvata lugens (Stål), Using DALP-PCR Molecular Markers
title_short Genetic Dissection of Sympatric Populations of Brown Planthopper, Nilaparvata lugens (Stål), Using DALP-PCR Molecular Markers
title_sort genetic dissection of sympatric populations of brown planthopper, nilaparvata lugens (stål), using dalp-pcr molecular markers
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3346741/
https://www.ncbi.nlm.nih.gov/pubmed/22593700
http://dx.doi.org/10.1100/2012/586831
work_keys_str_mv AT latifma geneticdissectionofsympatricpopulationsofbrownplanthoppernilaparvatalugensstalusingdalppcrmolecularmarkers
AT rafiimy geneticdissectionofsympatricpopulationsofbrownplanthoppernilaparvatalugensstalusingdalppcrmolecularmarkers
AT mazidms geneticdissectionofsympatricpopulationsofbrownplanthoppernilaparvatalugensstalusingdalppcrmolecularmarkers
AT alime geneticdissectionofsympatricpopulationsofbrownplanthoppernilaparvatalugensstalusingdalppcrmolecularmarkers
AT ahmedf geneticdissectionofsympatricpopulationsofbrownplanthoppernilaparvatalugensstalusingdalppcrmolecularmarkers
AT omarmy geneticdissectionofsympatricpopulationsofbrownplanthoppernilaparvatalugensstalusingdalppcrmolecularmarkers
AT tansg geneticdissectionofsympatricpopulationsofbrownplanthoppernilaparvatalugensstalusingdalppcrmolecularmarkers