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Flow-Based Systems for Rapid and High-Precision Enzyme Kinetics Studies

Enzyme kinetics studies normally focus on the initial rate of enzymatic reaction. However, the manual operation of steps of the conventional enzyme kinetics method has some drawbacks. Errors can result from the imprecise time control and time necessary for manual changing the reaction cuvettes into...

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Detalles Bibliográficos
Autores principales: Hartwell, Supaporn Kradtap, Grudpan, Kate
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3346984/
https://www.ncbi.nlm.nih.gov/pubmed/22577614
http://dx.doi.org/10.1155/2012/450716
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author Hartwell, Supaporn Kradtap
Grudpan, Kate
author_facet Hartwell, Supaporn Kradtap
Grudpan, Kate
author_sort Hartwell, Supaporn Kradtap
collection PubMed
description Enzyme kinetics studies normally focus on the initial rate of enzymatic reaction. However, the manual operation of steps of the conventional enzyme kinetics method has some drawbacks. Errors can result from the imprecise time control and time necessary for manual changing the reaction cuvettes into and out of the detector. By using the automatic flow-based analytical systems, enzyme kinetics studies can be carried out at real-time initial rate avoiding the potential errors inherent in manual operation. Flow-based systems have been developed to provide rapid, low-volume, and high-precision analyses that effectively replace the many tedious and high volume requirements of conventional wet chemistry analyses. This article presents various arrangements of flow-based techniques and their potential use in future enzyme kinetics applications.
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spelling pubmed-33469842012-05-10 Flow-Based Systems for Rapid and High-Precision Enzyme Kinetics Studies Hartwell, Supaporn Kradtap Grudpan, Kate J Anal Methods Chem Review Article Enzyme kinetics studies normally focus on the initial rate of enzymatic reaction. However, the manual operation of steps of the conventional enzyme kinetics method has some drawbacks. Errors can result from the imprecise time control and time necessary for manual changing the reaction cuvettes into and out of the detector. By using the automatic flow-based analytical systems, enzyme kinetics studies can be carried out at real-time initial rate avoiding the potential errors inherent in manual operation. Flow-based systems have been developed to provide rapid, low-volume, and high-precision analyses that effectively replace the many tedious and high volume requirements of conventional wet chemistry analyses. This article presents various arrangements of flow-based techniques and their potential use in future enzyme kinetics applications. Hindawi Publishing Corporation 2012 2012-04-22 /pmc/articles/PMC3346984/ /pubmed/22577614 http://dx.doi.org/10.1155/2012/450716 Text en Copyright © 2012 S. K. Hartwell and K. Grudpan. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Review Article
Hartwell, Supaporn Kradtap
Grudpan, Kate
Flow-Based Systems for Rapid and High-Precision Enzyme Kinetics Studies
title Flow-Based Systems for Rapid and High-Precision Enzyme Kinetics Studies
title_full Flow-Based Systems for Rapid and High-Precision Enzyme Kinetics Studies
title_fullStr Flow-Based Systems for Rapid and High-Precision Enzyme Kinetics Studies
title_full_unstemmed Flow-Based Systems for Rapid and High-Precision Enzyme Kinetics Studies
title_short Flow-Based Systems for Rapid and High-Precision Enzyme Kinetics Studies
title_sort flow-based systems for rapid and high-precision enzyme kinetics studies
topic Review Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3346984/
https://www.ncbi.nlm.nih.gov/pubmed/22577614
http://dx.doi.org/10.1155/2012/450716
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