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Flow-Based Systems for Rapid and High-Precision Enzyme Kinetics Studies
Enzyme kinetics studies normally focus on the initial rate of enzymatic reaction. However, the manual operation of steps of the conventional enzyme kinetics method has some drawbacks. Errors can result from the imprecise time control and time necessary for manual changing the reaction cuvettes into...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3346984/ https://www.ncbi.nlm.nih.gov/pubmed/22577614 http://dx.doi.org/10.1155/2012/450716 |
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author | Hartwell, Supaporn Kradtap Grudpan, Kate |
author_facet | Hartwell, Supaporn Kradtap Grudpan, Kate |
author_sort | Hartwell, Supaporn Kradtap |
collection | PubMed |
description | Enzyme kinetics studies normally focus on the initial rate of enzymatic reaction. However, the manual operation of steps of the conventional enzyme kinetics method has some drawbacks. Errors can result from the imprecise time control and time necessary for manual changing the reaction cuvettes into and out of the detector. By using the automatic flow-based analytical systems, enzyme kinetics studies can be carried out at real-time initial rate avoiding the potential errors inherent in manual operation. Flow-based systems have been developed to provide rapid, low-volume, and high-precision analyses that effectively replace the many tedious and high volume requirements of conventional wet chemistry analyses. This article presents various arrangements of flow-based techniques and their potential use in future enzyme kinetics applications. |
format | Online Article Text |
id | pubmed-3346984 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-33469842012-05-10 Flow-Based Systems for Rapid and High-Precision Enzyme Kinetics Studies Hartwell, Supaporn Kradtap Grudpan, Kate J Anal Methods Chem Review Article Enzyme kinetics studies normally focus on the initial rate of enzymatic reaction. However, the manual operation of steps of the conventional enzyme kinetics method has some drawbacks. Errors can result from the imprecise time control and time necessary for manual changing the reaction cuvettes into and out of the detector. By using the automatic flow-based analytical systems, enzyme kinetics studies can be carried out at real-time initial rate avoiding the potential errors inherent in manual operation. Flow-based systems have been developed to provide rapid, low-volume, and high-precision analyses that effectively replace the many tedious and high volume requirements of conventional wet chemistry analyses. This article presents various arrangements of flow-based techniques and their potential use in future enzyme kinetics applications. Hindawi Publishing Corporation 2012 2012-04-22 /pmc/articles/PMC3346984/ /pubmed/22577614 http://dx.doi.org/10.1155/2012/450716 Text en Copyright © 2012 S. K. Hartwell and K. Grudpan. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Review Article Hartwell, Supaporn Kradtap Grudpan, Kate Flow-Based Systems for Rapid and High-Precision Enzyme Kinetics Studies |
title | Flow-Based Systems for Rapid and High-Precision Enzyme Kinetics Studies |
title_full | Flow-Based Systems for Rapid and High-Precision Enzyme Kinetics Studies |
title_fullStr | Flow-Based Systems for Rapid and High-Precision Enzyme Kinetics Studies |
title_full_unstemmed | Flow-Based Systems for Rapid and High-Precision Enzyme Kinetics Studies |
title_short | Flow-Based Systems for Rapid and High-Precision Enzyme Kinetics Studies |
title_sort | flow-based systems for rapid and high-precision enzyme kinetics studies |
topic | Review Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3346984/ https://www.ncbi.nlm.nih.gov/pubmed/22577614 http://dx.doi.org/10.1155/2012/450716 |
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