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Telomerase Complex from Yeast Saccharomyces cerevisiae Contains a Biotinylated Component
Telomerase adds telomeric repeats to single-stranded DNA at the ends of the chromosomes. This enzyme is a ribonucleoprotein complex. Telomerase from yeast Saccharomyces cerevisiae consists of TLC1 RNA, which serves as a template for the synthesis of telomeric repeats, telomerase reverse transcriptas...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
A.I. Gordeyev
2009
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3347513/ https://www.ncbi.nlm.nih.gov/pubmed/22649607 |
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author | Shcherbakova, D.M. Zvereva, M.I. Dontsova, O.A. |
author_facet | Shcherbakova, D.M. Zvereva, M.I. Dontsova, O.A. |
author_sort | Shcherbakova, D.M. |
collection | PubMed |
description | Telomerase adds telomeric repeats to single-stranded DNA at the ends of the chromosomes. This enzyme is a ribonucleoprotein complex. Telomerase from yeast Saccharomyces cerevisiae consists of TLC1 RNA, which serves as a template for the synthesis of telomeric repeats, telomerase reverse transcriptase Est2p, and a number of accessory proteins (Est1p, Est3p, Ku70/Ku80, and Sm-complex). We found that the yeast telomerase complex contains a biotinylated component. The telomerase fraction containing biotinylated protein is active in vitro and constitutes a small part of the total amount of active telomerase isolated from cells. We speculate about the nature of the biotinylated component. |
format | Online Article Text |
id | pubmed-3347513 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
publisher | A.I. Gordeyev |
record_format | MEDLINE/PubMed |
spelling | pubmed-33475132012-05-30 Telomerase Complex from Yeast Saccharomyces cerevisiae Contains a Biotinylated Component Shcherbakova, D.M. Zvereva, M.I. Dontsova, O.A. Acta Naturae Research Article Telomerase adds telomeric repeats to single-stranded DNA at the ends of the chromosomes. This enzyme is a ribonucleoprotein complex. Telomerase from yeast Saccharomyces cerevisiae consists of TLC1 RNA, which serves as a template for the synthesis of telomeric repeats, telomerase reverse transcriptase Est2p, and a number of accessory proteins (Est1p, Est3p, Ku70/Ku80, and Sm-complex). We found that the yeast telomerase complex contains a biotinylated component. The telomerase fraction containing biotinylated protein is active in vitro and constitutes a small part of the total amount of active telomerase isolated from cells. We speculate about the nature of the biotinylated component. A.I. Gordeyev 2009-07 /pmc/articles/PMC3347513/ /pubmed/22649607 Text en Copyright © 2009 Park-media Ltd. http://creativecommons.org/licenses/by/2.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Shcherbakova, D.M. Zvereva, M.I. Dontsova, O.A. Telomerase Complex from Yeast Saccharomyces cerevisiae Contains a Biotinylated Component |
title | Telomerase Complex from Yeast Saccharomyces cerevisiae Contains a Biotinylated Component |
title_full | Telomerase Complex from Yeast Saccharomyces cerevisiae Contains a Biotinylated Component |
title_fullStr | Telomerase Complex from Yeast Saccharomyces cerevisiae Contains a Biotinylated Component |
title_full_unstemmed | Telomerase Complex from Yeast Saccharomyces cerevisiae Contains a Biotinylated Component |
title_short | Telomerase Complex from Yeast Saccharomyces cerevisiae Contains a Biotinylated Component |
title_sort | telomerase complex from yeast saccharomyces cerevisiae contains a biotinylated component |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3347513/ https://www.ncbi.nlm.nih.gov/pubmed/22649607 |
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