Telomerase Complex from Yeast Saccharomyces cerevisiae Contains a Biotinylated Component

Telomerase adds telomeric repeats to single-stranded DNA at the ends of the chromosomes. This enzyme is a ribonucleoprotein complex. Telomerase from yeast Saccharomyces cerevisiae consists of TLC1 RNA, which serves as a template for the synthesis of telomeric repeats, telomerase reverse transcriptas...

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Autores principales: Shcherbakova, D.M., Zvereva, M.I., Dontsova, O.A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: A.I. Gordeyev 2009
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3347513/
https://www.ncbi.nlm.nih.gov/pubmed/22649607
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author Shcherbakova, D.M.
Zvereva, M.I.
Dontsova, O.A.
author_facet Shcherbakova, D.M.
Zvereva, M.I.
Dontsova, O.A.
author_sort Shcherbakova, D.M.
collection PubMed
description Telomerase adds telomeric repeats to single-stranded DNA at the ends of the chromosomes. This enzyme is a ribonucleoprotein complex. Telomerase from yeast Saccharomyces cerevisiae consists of TLC1 RNA, which serves as a template for the synthesis of telomeric repeats, telomerase reverse transcriptase Est2p, and a number of accessory proteins (Est1p, Est3p, Ku70/Ku80, and Sm-complex). We found that the yeast telomerase complex contains a biotinylated component. The telomerase fraction containing biotinylated protein is active in vitro and constitutes a small part of the total amount of active telomerase isolated from cells. We speculate about the nature of the biotinylated component.
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spelling pubmed-33475132012-05-30 Telomerase Complex from Yeast Saccharomyces cerevisiae Contains a Biotinylated Component Shcherbakova, D.M. Zvereva, M.I. Dontsova, O.A. Acta Naturae Research Article Telomerase adds telomeric repeats to single-stranded DNA at the ends of the chromosomes. This enzyme is a ribonucleoprotein complex. Telomerase from yeast Saccharomyces cerevisiae consists of TLC1 RNA, which serves as a template for the synthesis of telomeric repeats, telomerase reverse transcriptase Est2p, and a number of accessory proteins (Est1p, Est3p, Ku70/Ku80, and Sm-complex). We found that the yeast telomerase complex contains a biotinylated component. The telomerase fraction containing biotinylated protein is active in vitro and constitutes a small part of the total amount of active telomerase isolated from cells. We speculate about the nature of the biotinylated component. A.I. Gordeyev 2009-07 /pmc/articles/PMC3347513/ /pubmed/22649607 Text en Copyright © 2009 Park-media Ltd. http://creativecommons.org/licenses/by/2.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Shcherbakova, D.M.
Zvereva, M.I.
Dontsova, O.A.
Telomerase Complex from Yeast Saccharomyces cerevisiae Contains a Biotinylated Component
title Telomerase Complex from Yeast Saccharomyces cerevisiae Contains a Biotinylated Component
title_full Telomerase Complex from Yeast Saccharomyces cerevisiae Contains a Biotinylated Component
title_fullStr Telomerase Complex from Yeast Saccharomyces cerevisiae Contains a Biotinylated Component
title_full_unstemmed Telomerase Complex from Yeast Saccharomyces cerevisiae Contains a Biotinylated Component
title_short Telomerase Complex from Yeast Saccharomyces cerevisiae Contains a Biotinylated Component
title_sort telomerase complex from yeast saccharomyces cerevisiae contains a biotinylated component
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3347513/
https://www.ncbi.nlm.nih.gov/pubmed/22649607
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