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Induction of a Protective Heterosubtypic Immune Response Against the Influenza Virus by using Recombinant Adenoviral Vectors Expressing Hemagglutinin of the Influenza H5 Virus

Abstract Influenza viruses are characterized by a high degree of antigenic variability, which causes the annual emergence of flu epidemics and irregularly timed pandemics caused by viruses with new antigenic and biological traits. Novel approaches to vaccination can help circumvent this problem. One...

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Autores principales: Shmarov, M.M., Sedova, E.S., Verkhovskaya, L.V., Rudneva, I.A., Bogacheva, E.A., Barykova, Yu.A., Shcherbinin, D.N., Lysenko, A.A., Tutykhina, I.L., Logunov, D.Y., Smirnov, Yu.A., Naroditsky, B.S., Gintsburg, A.L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: A.I. Gordeyev 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3347542/
https://www.ncbi.nlm.nih.gov/pubmed/22649637
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author Shmarov, M.M.
Sedova, E.S.
Verkhovskaya, L.V.
Rudneva, I.A.
Bogacheva, E.A.
Barykova, Yu.A.
Shcherbinin, D.N.
Lysenko, A.A.
Tutykhina, I.L.
Logunov, D.Y.
Smirnov, Yu.A.
Naroditsky, B.S.
Gintsburg, A.L.
author_facet Shmarov, M.M.
Sedova, E.S.
Verkhovskaya, L.V.
Rudneva, I.A.
Bogacheva, E.A.
Barykova, Yu.A.
Shcherbinin, D.N.
Lysenko, A.A.
Tutykhina, I.L.
Logunov, D.Y.
Smirnov, Yu.A.
Naroditsky, B.S.
Gintsburg, A.L.
author_sort Shmarov, M.M.
collection PubMed
description Abstract Influenza viruses are characterized by a high degree of antigenic variability, which causes the annual emergence of flu epidemics and irregularly timed pandemics caused by viruses with new antigenic and biological traits. Novel approaches to vaccination can help circumvent this problem. One of these new methods incorporates genetic vaccines based on adenoviral vectors. Recombinant adenoviral vectors which contain hemagglutinin–encoding genes from avian H5N1 and H5N2 (Ad–HA5–1 and Ad–HA5–2) influenza viruses were obtained using the AdEasy Adenoviral Vector System (Stratagene). Laboratory mice received a double intranasal vaccination with Ad–HA5–1 and Ad–HA5–2. This study demonstrates that immunization with recombinant adenoviruses bearing the Н 5 influenza virus hemagglutinin gene induces a immune response which protects immunized mice from a lethal dose of the H5 influenza virus. Moreover, it also protects the host from a lethal dose of the H1 virus, which belongs to the same clade as H5, but does not confer protection from the subtype H3 influenza virus, which belongs to a different clade.
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spelling pubmed-33475422012-05-30 Induction of a Protective Heterosubtypic Immune Response Against the Influenza Virus by using Recombinant Adenoviral Vectors Expressing Hemagglutinin of the Influenza H5 Virus Shmarov, M.M. Sedova, E.S. Verkhovskaya, L.V. Rudneva, I.A. Bogacheva, E.A. Barykova, Yu.A. Shcherbinin, D.N. Lysenko, A.A. Tutykhina, I.L. Logunov, D.Y. Smirnov, Yu.A. Naroditsky, B.S. Gintsburg, A.L. Acta Naturae Research Article Abstract Influenza viruses are characterized by a high degree of antigenic variability, which causes the annual emergence of flu epidemics and irregularly timed pandemics caused by viruses with new antigenic and biological traits. Novel approaches to vaccination can help circumvent this problem. One of these new methods incorporates genetic vaccines based on adenoviral vectors. Recombinant adenoviral vectors which contain hemagglutinin–encoding genes from avian H5N1 and H5N2 (Ad–HA5–1 and Ad–HA5–2) influenza viruses were obtained using the AdEasy Adenoviral Vector System (Stratagene). Laboratory mice received a double intranasal vaccination with Ad–HA5–1 and Ad–HA5–2. This study demonstrates that immunization with recombinant adenoviruses bearing the Н 5 influenza virus hemagglutinin gene induces a immune response which protects immunized mice from a lethal dose of the H5 influenza virus. Moreover, it also protects the host from a lethal dose of the H1 virus, which belongs to the same clade as H5, but does not confer protection from the subtype H3 influenza virus, which belongs to a different clade. A.I. Gordeyev 2010-04 /pmc/articles/PMC3347542/ /pubmed/22649637 Text en Copyright © 2010 Park-media Ltd. http://creativecommons.org/licenses/by/2.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Shmarov, M.M.
Sedova, E.S.
Verkhovskaya, L.V.
Rudneva, I.A.
Bogacheva, E.A.
Barykova, Yu.A.
Shcherbinin, D.N.
Lysenko, A.A.
Tutykhina, I.L.
Logunov, D.Y.
Smirnov, Yu.A.
Naroditsky, B.S.
Gintsburg, A.L.
Induction of a Protective Heterosubtypic Immune Response Against the Influenza Virus by using Recombinant Adenoviral Vectors Expressing Hemagglutinin of the Influenza H5 Virus
title Induction of a Protective Heterosubtypic Immune Response Against the Influenza Virus by using Recombinant Adenoviral Vectors Expressing Hemagglutinin of the Influenza H5 Virus
title_full Induction of a Protective Heterosubtypic Immune Response Against the Influenza Virus by using Recombinant Adenoviral Vectors Expressing Hemagglutinin of the Influenza H5 Virus
title_fullStr Induction of a Protective Heterosubtypic Immune Response Against the Influenza Virus by using Recombinant Adenoviral Vectors Expressing Hemagglutinin of the Influenza H5 Virus
title_full_unstemmed Induction of a Protective Heterosubtypic Immune Response Against the Influenza Virus by using Recombinant Adenoviral Vectors Expressing Hemagglutinin of the Influenza H5 Virus
title_short Induction of a Protective Heterosubtypic Immune Response Against the Influenza Virus by using Recombinant Adenoviral Vectors Expressing Hemagglutinin of the Influenza H5 Virus
title_sort induction of a protective heterosubtypic immune response against the influenza virus by using recombinant adenoviral vectors expressing hemagglutinin of the influenza h5 virus
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3347542/
https://www.ncbi.nlm.nih.gov/pubmed/22649637
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