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Tumoricidal Activity of RNase A and DNase I
In our work the antitumor and antimetastatic activities of RNase A and DNase I were studied using two murine models of pulmonary (Lewis lung carcinoma) and liver (hepatoma A–1) metastases. We found that intramuscular administration of RNase A at the dose range of 0.1–50 µ g/kg retarded the primary t...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
A.I. Gordeyev
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3347544/ https://www.ncbi.nlm.nih.gov/pubmed/22649632 |
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author | Patutina, O.A. Mironova, N.L. Ryabchikova, E.I. Popova, N.A. Nikolin, V.P. Kaledin, V.I. Vlassov, V.V. Zenkova, M.A. |
author_facet | Patutina, O.A. Mironova, N.L. Ryabchikova, E.I. Popova, N.A. Nikolin, V.P. Kaledin, V.I. Vlassov, V.V. Zenkova, M.A. |
author_sort | Patutina, O.A. |
collection | PubMed |
description | In our work the antitumor and antimetastatic activities of RNase A and DNase I were studied using two murine models of pulmonary (Lewis lung carcinoma) and liver (hepatoma A–1) metastases. We found that intramuscular administration of RNase A at the dose range of 0.1–50 µ g/kg retarded the primary tumor growth by 20–40%, and this effect disappeared with the increase in RNase A dose over 0.5 mg/kg. DNase I showed no effect on the primary tumor growth. The intramuscular administration of RNase A (0.35–7 µ g/kg) or DNase I (0.02–2.3 mg/kg) resulted in a considerable decrease in the metastasis number into the lungs of animals with Lewis lung carcinoma and a decrease of the hepatic index of animals with hepatoma 1A. A histological analysis of the organs occupied by metastases revealed that the administration of RNase A and DNase I induced metastasis pathomorphism as manifested by the destruction of oncocytes, an increase in necrosis and apoptosis foci in metastases, and mononuclear infiltration. Our data indicated that RNase A and DNase I are highly promising as supplementary therapeutics for the treatment of metastasizing tumors. |
format | Online Article Text |
id | pubmed-3347544 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | A.I. Gordeyev |
record_format | MEDLINE/PubMed |
spelling | pubmed-33475442012-05-30 Tumoricidal Activity of RNase A and DNase I Patutina, O.A. Mironova, N.L. Ryabchikova, E.I. Popova, N.A. Nikolin, V.P. Kaledin, V.I. Vlassov, V.V. Zenkova, M.A. Acta Naturae Research Article In our work the antitumor and antimetastatic activities of RNase A and DNase I were studied using two murine models of pulmonary (Lewis lung carcinoma) and liver (hepatoma A–1) metastases. We found that intramuscular administration of RNase A at the dose range of 0.1–50 µ g/kg retarded the primary tumor growth by 20–40%, and this effect disappeared with the increase in RNase A dose over 0.5 mg/kg. DNase I showed no effect on the primary tumor growth. The intramuscular administration of RNase A (0.35–7 µ g/kg) or DNase I (0.02–2.3 mg/kg) resulted in a considerable decrease in the metastasis number into the lungs of animals with Lewis lung carcinoma and a decrease of the hepatic index of animals with hepatoma 1A. A histological analysis of the organs occupied by metastases revealed that the administration of RNase A and DNase I induced metastasis pathomorphism as manifested by the destruction of oncocytes, an increase in necrosis and apoptosis foci in metastases, and mononuclear infiltration. Our data indicated that RNase A and DNase I are highly promising as supplementary therapeutics for the treatment of metastasizing tumors. A.I. Gordeyev 2010-04 /pmc/articles/PMC3347544/ /pubmed/22649632 Text en Copyright © 2010 Park-media Ltd. http://creativecommons.org/licenses/by/2.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Patutina, O.A. Mironova, N.L. Ryabchikova, E.I. Popova, N.A. Nikolin, V.P. Kaledin, V.I. Vlassov, V.V. Zenkova, M.A. Tumoricidal Activity of RNase A and DNase I |
title | Tumoricidal Activity of RNase A and DNase I |
title_full | Tumoricidal Activity of RNase A and DNase I |
title_fullStr | Tumoricidal Activity of RNase A and DNase I |
title_full_unstemmed | Tumoricidal Activity of RNase A and DNase I |
title_short | Tumoricidal Activity of RNase A and DNase I |
title_sort | tumoricidal activity of rnase a and dnase i |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3347544/ https://www.ncbi.nlm.nih.gov/pubmed/22649632 |
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