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Tumoricidal Activity of RNase A and DNase I

In our work the antitumor and antimetastatic activities of RNase A and DNase I were studied using two murine models of pulmonary (Lewis lung carcinoma) and liver (hepatoma A–1) metastases. We found that intramuscular administration of RNase A at the dose range of 0.1–50 µ g/kg retarded the primary t...

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Autores principales: Patutina, O.A., Mironova, N.L., Ryabchikova, E.I., Popova, N.A., Nikolin, V.P., Kaledin, V.I., Vlassov, V.V., Zenkova, M.A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: A.I. Gordeyev 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3347544/
https://www.ncbi.nlm.nih.gov/pubmed/22649632
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author Patutina, O.A.
Mironova, N.L.
Ryabchikova, E.I.
Popova, N.A.
Nikolin, V.P.
Kaledin, V.I.
Vlassov, V.V.
Zenkova, M.A.
author_facet Patutina, O.A.
Mironova, N.L.
Ryabchikova, E.I.
Popova, N.A.
Nikolin, V.P.
Kaledin, V.I.
Vlassov, V.V.
Zenkova, M.A.
author_sort Patutina, O.A.
collection PubMed
description In our work the antitumor and antimetastatic activities of RNase A and DNase I were studied using two murine models of pulmonary (Lewis lung carcinoma) and liver (hepatoma A–1) metastases. We found that intramuscular administration of RNase A at the dose range of 0.1–50 µ g/kg retarded the primary tumor growth by 20–40%, and this effect disappeared with the increase in RNase A dose over 0.5 mg/kg. DNase I showed no effect on the primary tumor growth. The intramuscular administration of RNase A (0.35–7 µ g/kg) or DNase I (0.02–2.3 mg/kg) resulted in a considerable decrease in the metastasis number into the lungs of animals with Lewis lung carcinoma and a decrease of the hepatic index of animals with hepatoma 1A. A histological analysis of the organs occupied by metastases revealed that the administration of RNase A and DNase I induced metastasis pathomorphism as manifested by the destruction of oncocytes, an increase in necrosis and apoptosis foci in metastases, and mononuclear infiltration. Our data indicated that RNase A and DNase I are highly promising as supplementary therapeutics for the treatment of metastasizing tumors.
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spelling pubmed-33475442012-05-30 Tumoricidal Activity of RNase A and DNase I Patutina, O.A. Mironova, N.L. Ryabchikova, E.I. Popova, N.A. Nikolin, V.P. Kaledin, V.I. Vlassov, V.V. Zenkova, M.A. Acta Naturae Research Article In our work the antitumor and antimetastatic activities of RNase A and DNase I were studied using two murine models of pulmonary (Lewis lung carcinoma) and liver (hepatoma A–1) metastases. We found that intramuscular administration of RNase A at the dose range of 0.1–50 µ g/kg retarded the primary tumor growth by 20–40%, and this effect disappeared with the increase in RNase A dose over 0.5 mg/kg. DNase I showed no effect on the primary tumor growth. The intramuscular administration of RNase A (0.35–7 µ g/kg) or DNase I (0.02–2.3 mg/kg) resulted in a considerable decrease in the metastasis number into the lungs of animals with Lewis lung carcinoma and a decrease of the hepatic index of animals with hepatoma 1A. A histological analysis of the organs occupied by metastases revealed that the administration of RNase A and DNase I induced metastasis pathomorphism as manifested by the destruction of oncocytes, an increase in necrosis and apoptosis foci in metastases, and mononuclear infiltration. Our data indicated that RNase A and DNase I are highly promising as supplementary therapeutics for the treatment of metastasizing tumors. A.I. Gordeyev 2010-04 /pmc/articles/PMC3347544/ /pubmed/22649632 Text en Copyright © 2010 Park-media Ltd. http://creativecommons.org/licenses/by/2.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Patutina, O.A.
Mironova, N.L.
Ryabchikova, E.I.
Popova, N.A.
Nikolin, V.P.
Kaledin, V.I.
Vlassov, V.V.
Zenkova, M.A.
Tumoricidal Activity of RNase A and DNase I
title Tumoricidal Activity of RNase A and DNase I
title_full Tumoricidal Activity of RNase A and DNase I
title_fullStr Tumoricidal Activity of RNase A and DNase I
title_full_unstemmed Tumoricidal Activity of RNase A and DNase I
title_short Tumoricidal Activity of RNase A and DNase I
title_sort tumoricidal activity of rnase a and dnase i
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3347544/
https://www.ncbi.nlm.nih.gov/pubmed/22649632
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