Derivation of Induced Pluripotent Stem Cells from Fetal Human Skin Fibroblasts

The isolation and study of autologous human stem cells remain among the most urgent problems in cell biology and biomedicine to date. Induced pluripotent stem cells can be derived from human somatic cells by the overexpression of a number of genes. In this study we reprogrammed fetal human skin fibr...

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Detalles Bibliográficos
Autores principales: Medvedev, S.P., Malakhova, A.A., Grigor’eva, E.V., Shevchenko, A.I., Dementyeva, E.V., Sobolev, I.A., Lebedev, I.N., Shilov, A.G., Zhimulev, I.F., Zakian, S.M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: A.I. Gordeyev 2010
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3347555/
https://www.ncbi.nlm.nih.gov/pubmed/22649648
Descripción
Sumario:The isolation and study of autologous human stem cells remain among the most urgent problems in cell biology and biomedicine to date. Induced pluripotent stem cells can be derived from human somatic cells by the overexpression of a number of genes. In this study we reprogrammed fetal human skin fibroblasts by transduction with retroviral vectors carrying murine Oct4 , Sox2 , Klf4 , and c–Myc cDNAs. As a result, cells with the protein expression and gene transcription pattern characteristic of human embryonic stem cells were derived. These induced pluripotent cells are capable of differentiation in vitro into the ectoderm, mesoderm, and endoderm derivatives.

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