Derivation of Induced Pluripotent Stem Cells from Fetal Human Skin Fibroblasts

The isolation and study of autologous human stem cells remain among the most urgent problems in cell biology and biomedicine to date. Induced pluripotent stem cells can be derived from human somatic cells by the overexpression of a number of genes. In this study we reprogrammed fetal human skin fibr...

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Autores principales: Medvedev, S.P., Malakhova, A.A., Grigor’eva, E.V., Shevchenko, A.I., Dementyeva, E.V., Sobolev, I.A., Lebedev, I.N., Shilov, A.G., Zhimulev, I.F., Zakian, S.M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: A.I. Gordeyev 2010
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3347555/
https://www.ncbi.nlm.nih.gov/pubmed/22649648
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author Medvedev, S.P.
Malakhova, A.A.
Grigor’eva, E.V.
Shevchenko, A.I.
Dementyeva, E.V.
Sobolev, I.A.
Lebedev, I.N.
Shilov, A.G.
Zhimulev, I.F.
Zakian, S.M.
author_facet Medvedev, S.P.
Malakhova, A.A.
Grigor’eva, E.V.
Shevchenko, A.I.
Dementyeva, E.V.
Sobolev, I.A.
Lebedev, I.N.
Shilov, A.G.
Zhimulev, I.F.
Zakian, S.M.
author_sort Medvedev, S.P.
collection PubMed
description The isolation and study of autologous human stem cells remain among the most urgent problems in cell biology and biomedicine to date. Induced pluripotent stem cells can be derived from human somatic cells by the overexpression of a number of genes. In this study we reprogrammed fetal human skin fibroblasts by transduction with retroviral vectors carrying murine Oct4 , Sox2 , Klf4 , and c–Myc cDNAs. As a result, cells with the protein expression and gene transcription pattern characteristic of human embryonic stem cells were derived. These induced pluripotent cells are capable of differentiation in vitro into the ectoderm, mesoderm, and endoderm derivatives.
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spelling pubmed-33475552012-05-30 Derivation of Induced Pluripotent Stem Cells from Fetal Human Skin Fibroblasts Medvedev, S.P. Malakhova, A.A. Grigor’eva, E.V. Shevchenko, A.I. Dementyeva, E.V. Sobolev, I.A. Lebedev, I.N. Shilov, A.G. Zhimulev, I.F. Zakian, S.M. Acta Naturae Research Article The isolation and study of autologous human stem cells remain among the most urgent problems in cell biology and biomedicine to date. Induced pluripotent stem cells can be derived from human somatic cells by the overexpression of a number of genes. In this study we reprogrammed fetal human skin fibroblasts by transduction with retroviral vectors carrying murine Oct4 , Sox2 , Klf4 , and c–Myc cDNAs. As a result, cells with the protein expression and gene transcription pattern characteristic of human embryonic stem cells were derived. These induced pluripotent cells are capable of differentiation in vitro into the ectoderm, mesoderm, and endoderm derivatives. A.I. Gordeyev 2010-07 /pmc/articles/PMC3347555/ /pubmed/22649648 Text en Copyright © 2010 Park-media Ltd. http://creativecommons.org/licenses/by/2.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Medvedev, S.P.
Malakhova, A.A.
Grigor’eva, E.V.
Shevchenko, A.I.
Dementyeva, E.V.
Sobolev, I.A.
Lebedev, I.N.
Shilov, A.G.
Zhimulev, I.F.
Zakian, S.M.
Derivation of Induced Pluripotent Stem Cells from Fetal Human Skin Fibroblasts
title Derivation of Induced Pluripotent Stem Cells from Fetal Human Skin Fibroblasts
title_full Derivation of Induced Pluripotent Stem Cells from Fetal Human Skin Fibroblasts
title_fullStr Derivation of Induced Pluripotent Stem Cells from Fetal Human Skin Fibroblasts
title_full_unstemmed Derivation of Induced Pluripotent Stem Cells from Fetal Human Skin Fibroblasts
title_short Derivation of Induced Pluripotent Stem Cells from Fetal Human Skin Fibroblasts
title_sort derivation of induced pluripotent stem cells from fetal human skin fibroblasts
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3347555/
https://www.ncbi.nlm.nih.gov/pubmed/22649648
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