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Characteristics of Artificial Virus-like Particles Assembled in vitro from Potato Virus X Coat Protein and Foreign Viral RNAs

Potato virus X (PVX) and some other potexviruses can be reconstitutedin vitrofrom viral coat protein (CP) and RNA. PVX CP is capable of forming viral ribonucleoprotein complexes (vRNP) not only with homologous, but also with foreign RNAs. This paper presents the structure and properties of vRNP asse...

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Autores principales: Arkhipenko, M.V., Petrova, E.K., Nikitin, N.A., Protopopova, A.D., Dubrovin, E.V., Yaminskii, 
I.V., Rodionova, N.P., Karpova, O.V., Atabekov, J.G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: A.I. Gordeyev 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3347606/
https://www.ncbi.nlm.nih.gov/pubmed/22649692
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author Arkhipenko, M.V.
Petrova, E.K.
Nikitin, N.A.
Protopopova, A.D.
Dubrovin, E.V.
Yaminskii, 
I.V.
Rodionova, N.P.
Karpova, O.V.
Atabekov, J.G.
author_facet Arkhipenko, M.V.
Petrova, E.K.
Nikitin, N.A.
Protopopova, A.D.
Dubrovin, E.V.
Yaminskii, 
I.V.
Rodionova, N.P.
Karpova, O.V.
Atabekov, J.G.
author_sort Arkhipenko, M.V.
collection PubMed
description Potato virus X (PVX) and some other potexviruses can be reconstitutedin vitrofrom viral coat protein (CP) and RNA. PVX CP is capable of forming viral ribonucleoprotein complexes (vRNP) not only with homologous, but also with foreign RNAs. This paper presents the structure and properties of vRNP assembledin vitroupon incubation of PVX CP and RNAs of various plant and animal viruses belonging to different taxonomic groups. We have shown that the morphology and translational properties of vRNPs containing foreign (heterologous) RNA are identical to those of homological vRNP (PVX RNA – PVX CP). Our data suggest that the assembly of the “mixed” vRNPin vitrocould be started at the 5’-proximal region of the RNA, producing a helical structure of vRNPs with foreign nucleic acids. The formation of heterologous vRNPin vitrowith PVX CP appears not to require a specific 5’ end RNA nucleotide sequence, and the PVX CP seems to be able to pack foreign genetic material of various sizes and compositions into artificial virus-like particles.
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spelling pubmed-33476062012-05-30 Characteristics of Artificial Virus-like Particles Assembled in vitro from Potato Virus X Coat Protein and Foreign Viral RNAs Arkhipenko, M.V. Petrova, E.K. Nikitin, N.A. Protopopova, A.D. Dubrovin, E.V. Yaminskii, 
I.V. Rodionova, N.P. Karpova, O.V. Atabekov, J.G. Acta Naturae Research Article Potato virus X (PVX) and some other potexviruses can be reconstitutedin vitrofrom viral coat protein (CP) and RNA. PVX CP is capable of forming viral ribonucleoprotein complexes (vRNP) not only with homologous, but also with foreign RNAs. This paper presents the structure and properties of vRNP assembledin vitroupon incubation of PVX CP and RNAs of various plant and animal viruses belonging to different taxonomic groups. We have shown that the morphology and translational properties of vRNPs containing foreign (heterologous) RNA are identical to those of homological vRNP (PVX RNA – PVX CP). Our data suggest that the assembly of the “mixed” vRNPin vitrocould be started at the 5’-proximal region of the RNA, producing a helical structure of vRNPs with foreign nucleic acids. The formation of heterologous vRNPin vitrowith PVX CP appears not to require a specific 5’ end RNA nucleotide sequence, and the PVX CP seems to be able to pack foreign genetic material of various sizes and compositions into artificial virus-like particles. A.I. Gordeyev 2011 /pmc/articles/PMC3347606/ /pubmed/22649692 Text en Copyright © 2011 Park-media Ltd. http://creativecommons.org/licenses/by/2.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Arkhipenko, M.V.
Petrova, E.K.
Nikitin, N.A.
Protopopova, A.D.
Dubrovin, E.V.
Yaminskii, 
I.V.
Rodionova, N.P.
Karpova, O.V.
Atabekov, J.G.
Characteristics of Artificial Virus-like Particles Assembled in vitro from Potato Virus X Coat Protein and Foreign Viral RNAs
title Characteristics of Artificial Virus-like Particles Assembled in vitro from Potato Virus X Coat Protein and Foreign Viral RNAs
title_full Characteristics of Artificial Virus-like Particles Assembled in vitro from Potato Virus X Coat Protein and Foreign Viral RNAs
title_fullStr Characteristics of Artificial Virus-like Particles Assembled in vitro from Potato Virus X Coat Protein and Foreign Viral RNAs
title_full_unstemmed Characteristics of Artificial Virus-like Particles Assembled in vitro from Potato Virus X Coat Protein and Foreign Viral RNAs
title_short Characteristics of Artificial Virus-like Particles Assembled in vitro from Potato Virus X Coat Protein and Foreign Viral RNAs
title_sort characteristics of artificial virus-like particles assembled in vitro from potato virus x coat protein and foreign viral rnas
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3347606/
https://www.ncbi.nlm.nih.gov/pubmed/22649692
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