RP-HPLC Method Development and Its Validation for Quantitative Determination of Rimonabant in Human Plasma

A simple, accurate, and precise HPLC method was developed and validated for determination of rimonabant in human plasma. Following liquid-liquid extraction, chromatographic separation was accomplished using C18 column with mobile phase consisting of acetonitrile : water (90 : 10, v/v), drug was dete...

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Detalles Bibliográficos
Autores principales: Bankey, Shravan, Tapadiya, Ganesh, Lamale, Jasvant, Jain, Deepti, Saboo, Shweta, Khadabadi, S. S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2012
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3347727/
https://www.ncbi.nlm.nih.gov/pubmed/22619744
http://dx.doi.org/10.1155/2012/625979
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author Bankey, Shravan
Tapadiya, Ganesh
Lamale, Jasvant
Jain, Deepti
Saboo, Shweta
Khadabadi, S. S.
author_facet Bankey, Shravan
Tapadiya, Ganesh
Lamale, Jasvant
Jain, Deepti
Saboo, Shweta
Khadabadi, S. S.
author_sort Bankey, Shravan
collection PubMed
description A simple, accurate, and precise HPLC method was developed and validated for determination of rimonabant in human plasma. Following liquid-liquid extraction, chromatographic separation was accomplished using C18 column with mobile phase consisting of acetonitrile : water (90 : 10, v/v), drug was detected at 260 nm using UVdetector. The LOD and LOQ were 3.0 and 10.0 μg/L, respectively. The method is linear in the interval 50.0–1000.0 μg/L. The average extraction recovery of drug from plasma was found to be 92.2%. The percent CV of the method was found to be less than 10.8%, and accuracy was found between 94.5 and 106.7%. The assay may be applied to a pharmacokinetic and bioequivalence study of rimonabant.
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spelling pubmed-33477272012-05-22 RP-HPLC Method Development and Its Validation for Quantitative Determination of Rimonabant in Human Plasma Bankey, Shravan Tapadiya, Ganesh Lamale, Jasvant Jain, Deepti Saboo, Shweta Khadabadi, S. S. J Anal Methods Chem Research Article A simple, accurate, and precise HPLC method was developed and validated for determination of rimonabant in human plasma. Following liquid-liquid extraction, chromatographic separation was accomplished using C18 column with mobile phase consisting of acetonitrile : water (90 : 10, v/v), drug was detected at 260 nm using UVdetector. The LOD and LOQ were 3.0 and 10.0 μg/L, respectively. The method is linear in the interval 50.0–1000.0 μg/L. The average extraction recovery of drug from plasma was found to be 92.2%. The percent CV of the method was found to be less than 10.8%, and accuracy was found between 94.5 and 106.7%. The assay may be applied to a pharmacokinetic and bioequivalence study of rimonabant. Hindawi Publishing Corporation 2012 2012-04-23 /pmc/articles/PMC3347727/ /pubmed/22619744 http://dx.doi.org/10.1155/2012/625979 Text en Copyright © 2012 Shravan Bankey et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Bankey, Shravan
Tapadiya, Ganesh
Lamale, Jasvant
Jain, Deepti
Saboo, Shweta
Khadabadi, S. S.
RP-HPLC Method Development and Its Validation for Quantitative Determination of Rimonabant in Human Plasma
title RP-HPLC Method Development and Its Validation for Quantitative Determination of Rimonabant in Human Plasma
title_full RP-HPLC Method Development and Its Validation for Quantitative Determination of Rimonabant in Human Plasma
title_fullStr RP-HPLC Method Development and Its Validation for Quantitative Determination of Rimonabant in Human Plasma
title_full_unstemmed RP-HPLC Method Development and Its Validation for Quantitative Determination of Rimonabant in Human Plasma
title_short RP-HPLC Method Development and Its Validation for Quantitative Determination of Rimonabant in Human Plasma
title_sort rp-hplc method development and its validation for quantitative determination of rimonabant in human plasma
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3347727/
https://www.ncbi.nlm.nih.gov/pubmed/22619744
http://dx.doi.org/10.1155/2012/625979
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