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NK Cells Promote Th-17 Mediated Corneal Barrier Disruption in Dry Eye

BACKGROUND: The conjunctiva contains a specialized population of lymphocytes that reside in the epithelium, named intraepithelial lymphocytes (IEL). METHODOLOGY/PRINCIPAL FINDINGS: Here we characterized the IEL population prior to and after experimental desiccating stress (DS) for 5 or 10 days (DS5,...

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Autores principales: Zhang, Xiaobo, Volpe, Eugene A., Gandhi, Niral B., Schaumburg, Chris S., Siemasko, Karyn F., Pangelinan, Solherny B., Kelly, Scott D., Hayday, Adrian C., Li, De-Quan, Stern, Michael E., Niederkorn, Jerry Y., Pflugfelder, Stephen C., De Paiva, Cintia S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3348128/
https://www.ncbi.nlm.nih.gov/pubmed/22590618
http://dx.doi.org/10.1371/journal.pone.0036822
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author Zhang, Xiaobo
Volpe, Eugene A.
Gandhi, Niral B.
Schaumburg, Chris S.
Siemasko, Karyn F.
Pangelinan, Solherny B.
Kelly, Scott D.
Hayday, Adrian C.
Li, De-Quan
Stern, Michael E.
Niederkorn, Jerry Y.
Pflugfelder, Stephen C.
De Paiva, Cintia S.
author_facet Zhang, Xiaobo
Volpe, Eugene A.
Gandhi, Niral B.
Schaumburg, Chris S.
Siemasko, Karyn F.
Pangelinan, Solherny B.
Kelly, Scott D.
Hayday, Adrian C.
Li, De-Quan
Stern, Michael E.
Niederkorn, Jerry Y.
Pflugfelder, Stephen C.
De Paiva, Cintia S.
author_sort Zhang, Xiaobo
collection PubMed
description BACKGROUND: The conjunctiva contains a specialized population of lymphocytes that reside in the epithelium, named intraepithelial lymphocytes (IEL). METHODOLOGY/PRINCIPAL FINDINGS: Here we characterized the IEL population prior to and after experimental desiccating stress (DS) for 5 or 10 days (DS5, DS10) and evaluated the effect of NK depletion on DS. The frequency of IELs in normal murine conjunctiva was CD3(+)CD103(+) (∼22%), CD3(+)γδ(+) (∼9.6%), CD3(+)NK(+) (2%), CD3(−)NK(+) (∼4.4%), CD3(+)CD8α (∼0.9%), and CD4 (∼0.6%). Systemic depletion of NK cells prior and during DS led to a decrease in the frequency of total and activated DCs, a decrease in T helper-17(+) cells in the cervical lymph nodes and generation of less pathogenic CD4(+)T cells. B6.nude recipient mice of adoptively transferred CD4(+)T cells isolated from NK-depleted DS5 donor mice showed significantly less corneal barrier disruption, lower levels of IL-17A, CCL20 and MMP-3 in the cornea epithelia compared to recipients of control CD4(+)T cells. CONCLUSIONS/SIGNIFICANCE: Taken together, these results show that the NK IELs are involved in the acute immune response to desiccation-induced dry eye by activating DC, which in turn coordinate generation of the pathogenic Th-17 response.
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spelling pubmed-33481282012-05-15 NK Cells Promote Th-17 Mediated Corneal Barrier Disruption in Dry Eye Zhang, Xiaobo Volpe, Eugene A. Gandhi, Niral B. Schaumburg, Chris S. Siemasko, Karyn F. Pangelinan, Solherny B. Kelly, Scott D. Hayday, Adrian C. Li, De-Quan Stern, Michael E. Niederkorn, Jerry Y. Pflugfelder, Stephen C. De Paiva, Cintia S. PLoS One Research Article BACKGROUND: The conjunctiva contains a specialized population of lymphocytes that reside in the epithelium, named intraepithelial lymphocytes (IEL). METHODOLOGY/PRINCIPAL FINDINGS: Here we characterized the IEL population prior to and after experimental desiccating stress (DS) for 5 or 10 days (DS5, DS10) and evaluated the effect of NK depletion on DS. The frequency of IELs in normal murine conjunctiva was CD3(+)CD103(+) (∼22%), CD3(+)γδ(+) (∼9.6%), CD3(+)NK(+) (2%), CD3(−)NK(+) (∼4.4%), CD3(+)CD8α (∼0.9%), and CD4 (∼0.6%). Systemic depletion of NK cells prior and during DS led to a decrease in the frequency of total and activated DCs, a decrease in T helper-17(+) cells in the cervical lymph nodes and generation of less pathogenic CD4(+)T cells. B6.nude recipient mice of adoptively transferred CD4(+)T cells isolated from NK-depleted DS5 donor mice showed significantly less corneal barrier disruption, lower levels of IL-17A, CCL20 and MMP-3 in the cornea epithelia compared to recipients of control CD4(+)T cells. CONCLUSIONS/SIGNIFICANCE: Taken together, these results show that the NK IELs are involved in the acute immune response to desiccation-induced dry eye by activating DC, which in turn coordinate generation of the pathogenic Th-17 response. Public Library of Science 2012-05-08 /pmc/articles/PMC3348128/ /pubmed/22590618 http://dx.doi.org/10.1371/journal.pone.0036822 Text en Zhang et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Zhang, Xiaobo
Volpe, Eugene A.
Gandhi, Niral B.
Schaumburg, Chris S.
Siemasko, Karyn F.
Pangelinan, Solherny B.
Kelly, Scott D.
Hayday, Adrian C.
Li, De-Quan
Stern, Michael E.
Niederkorn, Jerry Y.
Pflugfelder, Stephen C.
De Paiva, Cintia S.
NK Cells Promote Th-17 Mediated Corneal Barrier Disruption in Dry Eye
title NK Cells Promote Th-17 Mediated Corneal Barrier Disruption in Dry Eye
title_full NK Cells Promote Th-17 Mediated Corneal Barrier Disruption in Dry Eye
title_fullStr NK Cells Promote Th-17 Mediated Corneal Barrier Disruption in Dry Eye
title_full_unstemmed NK Cells Promote Th-17 Mediated Corneal Barrier Disruption in Dry Eye
title_short NK Cells Promote Th-17 Mediated Corneal Barrier Disruption in Dry Eye
title_sort nk cells promote th-17 mediated corneal barrier disruption in dry eye
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3348128/
https://www.ncbi.nlm.nih.gov/pubmed/22590618
http://dx.doi.org/10.1371/journal.pone.0036822
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