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Interleukin 28B Gene Polymorphism and Association with Chronic Hepatitis C Therapy Results in Latvia

Introduction. With the standard treatment of chronic hepatitis C, sustained virological response (SVR) can be achieved only in half of all patients. Interleukin-28B appears to be involved in the control of HCV infection, and the genetic polymorphism of the encoding IL-28B gene may determine the effi...

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Autores principales: Tolmane, Ieva, Rozentale, Baiba, Keiss, Jazeps, Ivancenko, Ludmila, Subnikova, Nadezda, Reinholde, Zaiga, Kozlovska, Ieva, Sumlaninova, Nina, Laivacuma, Sniedze, Simanis, Raimonds
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3348628/
https://www.ncbi.nlm.nih.gov/pubmed/22619706
http://dx.doi.org/10.1155/2012/324090
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author Tolmane, Ieva
Rozentale, Baiba
Keiss, Jazeps
Ivancenko, Ludmila
Subnikova, Nadezda
Reinholde, Zaiga
Kozlovska, Ieva
Sumlaninova, Nina
Laivacuma, Sniedze
Simanis, Raimonds
author_facet Tolmane, Ieva
Rozentale, Baiba
Keiss, Jazeps
Ivancenko, Ludmila
Subnikova, Nadezda
Reinholde, Zaiga
Kozlovska, Ieva
Sumlaninova, Nina
Laivacuma, Sniedze
Simanis, Raimonds
author_sort Tolmane, Ieva
collection PubMed
description Introduction. With the standard treatment of chronic hepatitis C, sustained virological response (SVR) can be achieved only in half of all patients. Interleukin-28B appears to be involved in the control of HCV infection, and the genetic polymorphism of the encoding IL-28B gene may determine the efficacy of clearance of HCV. The aim of this paper was to detect IL-28B gene polymorphism in Latvia and to analyze therapy results. This is the first study on IL-28B gene polymorphism in Latvia. Material and Methods. There were 159 chronic viral hepatitis C patients included in the study. In order to detect IL-28B gene polymorphism, we used molecular biology techniques and methods: classical DNA separation, amplification by PCR, and standard sequencing. Genotype was defined as CC, CT, TC, or TT type. 142 patients were treated with the standard of care treatment. Results were analyzed according to IL-28B polymorphism. Results. There were 53 patients (33%) with CC genotype, 84 patients (53%) with CT/TC genotype, and 22 patients (14%) with TT genotype. 34 patients (74%) in CC genotype subgroup achieved SVR versus 50 patients (52%) in non-CC subgroups. In patients with genotype 1, SVR was achieved in 16 patients (84%) in CC subgroup versus 30 patients (47.6%) in non-CC subgroups, P = 0.007. Conclusions. The most common genotype of IL28B in Latvia is CT/TC, with an incidence of 53%. Patients with CC genotype achieved SVR more often than CT or TT subgroups. IL28B gene polymorphism therefore is a strong predictor of treatment result.
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spelling pubmed-33486282012-05-22 Interleukin 28B Gene Polymorphism and Association with Chronic Hepatitis C Therapy Results in Latvia Tolmane, Ieva Rozentale, Baiba Keiss, Jazeps Ivancenko, Ludmila Subnikova, Nadezda Reinholde, Zaiga Kozlovska, Ieva Sumlaninova, Nina Laivacuma, Sniedze Simanis, Raimonds Hepat Res Treat Research Article Introduction. With the standard treatment of chronic hepatitis C, sustained virological response (SVR) can be achieved only in half of all patients. Interleukin-28B appears to be involved in the control of HCV infection, and the genetic polymorphism of the encoding IL-28B gene may determine the efficacy of clearance of HCV. The aim of this paper was to detect IL-28B gene polymorphism in Latvia and to analyze therapy results. This is the first study on IL-28B gene polymorphism in Latvia. Material and Methods. There were 159 chronic viral hepatitis C patients included in the study. In order to detect IL-28B gene polymorphism, we used molecular biology techniques and methods: classical DNA separation, amplification by PCR, and standard sequencing. Genotype was defined as CC, CT, TC, or TT type. 142 patients were treated with the standard of care treatment. Results were analyzed according to IL-28B polymorphism. Results. There were 53 patients (33%) with CC genotype, 84 patients (53%) with CT/TC genotype, and 22 patients (14%) with TT genotype. 34 patients (74%) in CC genotype subgroup achieved SVR versus 50 patients (52%) in non-CC subgroups. In patients with genotype 1, SVR was achieved in 16 patients (84%) in CC subgroup versus 30 patients (47.6%) in non-CC subgroups, P = 0.007. Conclusions. The most common genotype of IL28B in Latvia is CT/TC, with an incidence of 53%. Patients with CC genotype achieved SVR more often than CT or TT subgroups. IL28B gene polymorphism therefore is a strong predictor of treatment result. Hindawi Publishing Corporation 2012 2012-04-24 /pmc/articles/PMC3348628/ /pubmed/22619706 http://dx.doi.org/10.1155/2012/324090 Text en Copyright © 2012 Ieva Tolmane et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Tolmane, Ieva
Rozentale, Baiba
Keiss, Jazeps
Ivancenko, Ludmila
Subnikova, Nadezda
Reinholde, Zaiga
Kozlovska, Ieva
Sumlaninova, Nina
Laivacuma, Sniedze
Simanis, Raimonds
Interleukin 28B Gene Polymorphism and Association with Chronic Hepatitis C Therapy Results in Latvia
title Interleukin 28B Gene Polymorphism and Association with Chronic Hepatitis C Therapy Results in Latvia
title_full Interleukin 28B Gene Polymorphism and Association with Chronic Hepatitis C Therapy Results in Latvia
title_fullStr Interleukin 28B Gene Polymorphism and Association with Chronic Hepatitis C Therapy Results in Latvia
title_full_unstemmed Interleukin 28B Gene Polymorphism and Association with Chronic Hepatitis C Therapy Results in Latvia
title_short Interleukin 28B Gene Polymorphism and Association with Chronic Hepatitis C Therapy Results in Latvia
title_sort interleukin 28b gene polymorphism and association with chronic hepatitis c therapy results in latvia
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3348628/
https://www.ncbi.nlm.nih.gov/pubmed/22619706
http://dx.doi.org/10.1155/2012/324090
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