An Adaptable, Portable Microarray Reader for Biodetection

We have developed an inexpensive portable microarray reader that can be applied to standard microscope slide-based arrays and other array formats printed on chemically modified surfaces. Measuring only 19 cm in length, the imaging device is portable and may be applicable to both triage and clinical...

Descripción completa

Detalles Bibliográficos
Autores principales: Thompson, Deanna L., Pearson, Francesca, Thomas, Cynthia, Rao, Rupa, Matthews, Dennis, Albala, Joanna S., Wachsmann-Hogiu, Sebastian, Coleman, Matthew A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2009
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3348790/
https://www.ncbi.nlm.nih.gov/pubmed/22574030
http://dx.doi.org/10.3390/s90402524
_version_ 1782232419304210432
author Thompson, Deanna L.
Pearson, Francesca
Thomas, Cynthia
Rao, Rupa
Matthews, Dennis
Albala, Joanna S.
Wachsmann-Hogiu, Sebastian
Coleman, Matthew A.
author_facet Thompson, Deanna L.
Pearson, Francesca
Thomas, Cynthia
Rao, Rupa
Matthews, Dennis
Albala, Joanna S.
Wachsmann-Hogiu, Sebastian
Coleman, Matthew A.
author_sort Thompson, Deanna L.
collection PubMed
description We have developed an inexpensive portable microarray reader that can be applied to standard microscope slide-based arrays and other array formats printed on chemically modified surfaces. Measuring only 19 cm in length, the imaging device is portable and may be applicable to both triage and clinical settings. For multiplexing and adaptability purposes, it can be modified to work with multiple excitation/emission wavelengths. Our device is shown to be comparable to a commercial laser scanner when detecting both streptavidin-biotin and antibody interactions. This paper presents the development and characterization of a handheld microarray imager and directly compares it with a commercial scanner.
format Online
Article
Text
id pubmed-3348790
institution National Center for Biotechnology Information
language English
publishDate 2009
publisher Molecular Diversity Preservation International (MDPI)
record_format MEDLINE/PubMed
spelling pubmed-33487902012-05-09 An Adaptable, Portable Microarray Reader for Biodetection Thompson, Deanna L. Pearson, Francesca Thomas, Cynthia Rao, Rupa Matthews, Dennis Albala, Joanna S. Wachsmann-Hogiu, Sebastian Coleman, Matthew A. Sensors (Basel) Article We have developed an inexpensive portable microarray reader that can be applied to standard microscope slide-based arrays and other array formats printed on chemically modified surfaces. Measuring only 19 cm in length, the imaging device is portable and may be applicable to both triage and clinical settings. For multiplexing and adaptability purposes, it can be modified to work with multiple excitation/emission wavelengths. Our device is shown to be comparable to a commercial laser scanner when detecting both streptavidin-biotin and antibody interactions. This paper presents the development and characterization of a handheld microarray imager and directly compares it with a commercial scanner. Molecular Diversity Preservation International (MDPI) 2009-04-14 /pmc/articles/PMC3348790/ /pubmed/22574030 http://dx.doi.org/10.3390/s90402524 Text en © 2009 by the authors; licensee MDPI, Basel, Switzerland This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Thompson, Deanna L.
Pearson, Francesca
Thomas, Cynthia
Rao, Rupa
Matthews, Dennis
Albala, Joanna S.
Wachsmann-Hogiu, Sebastian
Coleman, Matthew A.
An Adaptable, Portable Microarray Reader for Biodetection
title An Adaptable, Portable Microarray Reader for Biodetection
title_full An Adaptable, Portable Microarray Reader for Biodetection
title_fullStr An Adaptable, Portable Microarray Reader for Biodetection
title_full_unstemmed An Adaptable, Portable Microarray Reader for Biodetection
title_short An Adaptable, Portable Microarray Reader for Biodetection
title_sort adaptable, portable microarray reader for biodetection
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3348790/
https://www.ncbi.nlm.nih.gov/pubmed/22574030
http://dx.doi.org/10.3390/s90402524
work_keys_str_mv AT thompsondeannal anadaptableportablemicroarrayreaderforbiodetection
AT pearsonfrancesca anadaptableportablemicroarrayreaderforbiodetection
AT thomascynthia anadaptableportablemicroarrayreaderforbiodetection
AT raorupa anadaptableportablemicroarrayreaderforbiodetection
AT matthewsdennis anadaptableportablemicroarrayreaderforbiodetection
AT albalajoannas anadaptableportablemicroarrayreaderforbiodetection
AT wachsmannhogiusebastian anadaptableportablemicroarrayreaderforbiodetection
AT colemanmatthewa anadaptableportablemicroarrayreaderforbiodetection
AT thompsondeannal adaptableportablemicroarrayreaderforbiodetection
AT pearsonfrancesca adaptableportablemicroarrayreaderforbiodetection
AT thomascynthia adaptableportablemicroarrayreaderforbiodetection
AT raorupa adaptableportablemicroarrayreaderforbiodetection
AT matthewsdennis adaptableportablemicroarrayreaderforbiodetection
AT albalajoannas adaptableportablemicroarrayreaderforbiodetection
AT wachsmannhogiusebastian adaptableportablemicroarrayreaderforbiodetection
AT colemanmatthewa adaptableportablemicroarrayreaderforbiodetection

Ejemplares similares