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Defluorination of Sodium Fluoroacetate by Bacteria from Soil and Plants in Brazil
The aim of this work was to isolate and identify bacteria able to degrade sodium fluoroacetate from soil and plant samples collected in areas where the fluoroacetate-containing plants Mascagnia rigida and Palicourea aenofusca are found. The samples were cultivated in mineral medium added with 20 mmo...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Scientific World Journal
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3349100/ https://www.ncbi.nlm.nih.gov/pubmed/22619595 http://dx.doi.org/10.1100/2012/149893 |
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author | Camboim, Expedito K. A. Tadra-Sfeir, Michelle Z. de Souza, Emanuel M. Pedrosa, Fabio de O. Andrade, Paulo P. McSweeney, Chris S. Riet-Correa, Franklin Melo, Marcia A. |
author_facet | Camboim, Expedito K. A. Tadra-Sfeir, Michelle Z. de Souza, Emanuel M. Pedrosa, Fabio de O. Andrade, Paulo P. McSweeney, Chris S. Riet-Correa, Franklin Melo, Marcia A. |
author_sort | Camboim, Expedito K. A. |
collection | PubMed |
description | The aim of this work was to isolate and identify bacteria able to degrade sodium fluoroacetate from soil and plant samples collected in areas where the fluoroacetate-containing plants Mascagnia rigida and Palicourea aenofusca are found. The samples were cultivated in mineral medium added with 20 mmol L(−1) sodium fluoroacetate. Seven isolates were identified by 16S rRNA gene sequencing as Paenibacillus sp. (ECPB01), Burkholderia sp. (ECPB02), Cupriavidus sp. (ECPB03), Staphylococcus sp. (ECPB04), Ancylobacter sp. (ECPB05), Ralstonia sp. (ECPB06), and Stenotrophomonas sp. (ECPB07). All seven isolates degraded sodium-fluoroacetate-containing in the medium, reaching defluorination rate of fluoride ion of 20 mmol L(−1). Six of them are reported for the first time as able to degrade sodium fluoroacetate (SF). In the future, some of these microorganisms can be used to establish in the rumen an engineered bacterial population able to degrade sodium fluoroacetate and protect ruminants from the poisoning by this compound. |
format | Online Article Text |
id | pubmed-3349100 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | The Scientific World Journal |
record_format | MEDLINE/PubMed |
spelling | pubmed-33491002012-05-22 Defluorination of Sodium Fluoroacetate by Bacteria from Soil and Plants in Brazil Camboim, Expedito K. A. Tadra-Sfeir, Michelle Z. de Souza, Emanuel M. Pedrosa, Fabio de O. Andrade, Paulo P. McSweeney, Chris S. Riet-Correa, Franklin Melo, Marcia A. ScientificWorldJournal Research Article The aim of this work was to isolate and identify bacteria able to degrade sodium fluoroacetate from soil and plant samples collected in areas where the fluoroacetate-containing plants Mascagnia rigida and Palicourea aenofusca are found. The samples were cultivated in mineral medium added with 20 mmol L(−1) sodium fluoroacetate. Seven isolates were identified by 16S rRNA gene sequencing as Paenibacillus sp. (ECPB01), Burkholderia sp. (ECPB02), Cupriavidus sp. (ECPB03), Staphylococcus sp. (ECPB04), Ancylobacter sp. (ECPB05), Ralstonia sp. (ECPB06), and Stenotrophomonas sp. (ECPB07). All seven isolates degraded sodium-fluoroacetate-containing in the medium, reaching defluorination rate of fluoride ion of 20 mmol L(−1). Six of them are reported for the first time as able to degrade sodium fluoroacetate (SF). In the future, some of these microorganisms can be used to establish in the rumen an engineered bacterial population able to degrade sodium fluoroacetate and protect ruminants from the poisoning by this compound. The Scientific World Journal 2012-04-24 /pmc/articles/PMC3349100/ /pubmed/22619595 http://dx.doi.org/10.1100/2012/149893 Text en Copyright © 2012 Expedito K. A. Camboim et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Camboim, Expedito K. A. Tadra-Sfeir, Michelle Z. de Souza, Emanuel M. Pedrosa, Fabio de O. Andrade, Paulo P. McSweeney, Chris S. Riet-Correa, Franklin Melo, Marcia A. Defluorination of Sodium Fluoroacetate by Bacteria from Soil and Plants in Brazil |
title | Defluorination of Sodium Fluoroacetate by Bacteria from Soil and Plants in Brazil |
title_full | Defluorination of Sodium Fluoroacetate by Bacteria from Soil and Plants in Brazil |
title_fullStr | Defluorination of Sodium Fluoroacetate by Bacteria from Soil and Plants in Brazil |
title_full_unstemmed | Defluorination of Sodium Fluoroacetate by Bacteria from Soil and Plants in Brazil |
title_short | Defluorination of Sodium Fluoroacetate by Bacteria from Soil and Plants in Brazil |
title_sort | defluorination of sodium fluoroacetate by bacteria from soil and plants in brazil |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3349100/ https://www.ncbi.nlm.nih.gov/pubmed/22619595 http://dx.doi.org/10.1100/2012/149893 |
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