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High Prevalence of Candida Yeast in Milk Samples from Cows Suffering from Mastitis in Poland

Mastitis is an economically important disease in which fungi belonging to the genus Candida may participate as etiological agents. This study focused on determining the frequency of fungal isolation and differentiation of fungal species isolated from milk of mastitic cows. Sixty-six milk samples fro...

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Autores principales: Dworecka-Kaszak, Bozena, Krutkiewicz, Alicja, Szopa, Daniel, Kleczkowski, Miroslaw, Biegańska, Malgorzata
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Scientific World Journal 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3349122/
https://www.ncbi.nlm.nih.gov/pubmed/22619625
http://dx.doi.org/10.1100/2012/196347
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author Dworecka-Kaszak, Bozena
Krutkiewicz, Alicja
Szopa, Daniel
Kleczkowski, Miroslaw
Biegańska, Malgorzata
author_facet Dworecka-Kaszak, Bozena
Krutkiewicz, Alicja
Szopa, Daniel
Kleczkowski, Miroslaw
Biegańska, Malgorzata
author_sort Dworecka-Kaszak, Bozena
collection PubMed
description Mastitis is an economically important disease in which fungi belonging to the genus Candida may participate as etiological agents. This study focused on determining the frequency of fungal isolation and differentiation of fungal species isolated from milk of mastitic cows. Sixty-six milk samples from mastitic cows were studied, and 55 strains of fungi were isolated. Seven different species classified as Candida were identified basing on phenotypic properties, and the dominating species was C. parapsilosis. Genomic DNA was isolated and amplified in PCR with ITS1 and NL2 primers. Amplification products were digested with restriction enzymes HpaII and EcoRI. Amplification of DNA with ITS1 and NL2 primers resulted in products of different sizes. Comparison of product sizes in restriction fragment PCR REA confirmed differences among species. Strains grouped together on the basis of phenotype characteristics differed in restriction fragment profiles. None of the investigated species showed similar genetic profiles.
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spelling pubmed-33491222012-05-22 High Prevalence of Candida Yeast in Milk Samples from Cows Suffering from Mastitis in Poland Dworecka-Kaszak, Bozena Krutkiewicz, Alicja Szopa, Daniel Kleczkowski, Miroslaw Biegańska, Malgorzata ScientificWorldJournal Research Article Mastitis is an economically important disease in which fungi belonging to the genus Candida may participate as etiological agents. This study focused on determining the frequency of fungal isolation and differentiation of fungal species isolated from milk of mastitic cows. Sixty-six milk samples from mastitic cows were studied, and 55 strains of fungi were isolated. Seven different species classified as Candida were identified basing on phenotypic properties, and the dominating species was C. parapsilosis. Genomic DNA was isolated and amplified in PCR with ITS1 and NL2 primers. Amplification products were digested with restriction enzymes HpaII and EcoRI. Amplification of DNA with ITS1 and NL2 primers resulted in products of different sizes. Comparison of product sizes in restriction fragment PCR REA confirmed differences among species. Strains grouped together on the basis of phenotype characteristics differed in restriction fragment profiles. None of the investigated species showed similar genetic profiles. The Scientific World Journal 2012-04-24 /pmc/articles/PMC3349122/ /pubmed/22619625 http://dx.doi.org/10.1100/2012/196347 Text en Copyright © 2012 Bozena Dworecka-Kaszak et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Dworecka-Kaszak, Bozena
Krutkiewicz, Alicja
Szopa, Daniel
Kleczkowski, Miroslaw
Biegańska, Malgorzata
High Prevalence of Candida Yeast in Milk Samples from Cows Suffering from Mastitis in Poland
title High Prevalence of Candida Yeast in Milk Samples from Cows Suffering from Mastitis in Poland
title_full High Prevalence of Candida Yeast in Milk Samples from Cows Suffering from Mastitis in Poland
title_fullStr High Prevalence of Candida Yeast in Milk Samples from Cows Suffering from Mastitis in Poland
title_full_unstemmed High Prevalence of Candida Yeast in Milk Samples from Cows Suffering from Mastitis in Poland
title_short High Prevalence of Candida Yeast in Milk Samples from Cows Suffering from Mastitis in Poland
title_sort high prevalence of candida yeast in milk samples from cows suffering from mastitis in poland
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3349122/
https://www.ncbi.nlm.nih.gov/pubmed/22619625
http://dx.doi.org/10.1100/2012/196347
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