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Characterization of the Immune Response Induced by a Commercially Available Inactivated Bluetongue Virus Serotype 1 Vaccine in Sheep
The protective immune response generated by a commercial monovalent inactivated vaccine against bluetongue virus serotype 1 (BTV1) was studied. Five sheep were vaccinated, boost-vaccinated, and then challenged against BTV1 ALG/2006. RT-PCR did not detect viremia at any time during the experiment. Ex...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Scientific World Journal
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3349316/ https://www.ncbi.nlm.nih.gov/pubmed/22619592 http://dx.doi.org/10.1100/2012/147158 |
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author | Pérez de Diego, Ana Cristina Sánchez-Cordón, Pedro José de las Heras, Ana Isabel Sánchez-Vizcaíno, José Manuel |
author_facet | Pérez de Diego, Ana Cristina Sánchez-Cordón, Pedro José de las Heras, Ana Isabel Sánchez-Vizcaíno, José Manuel |
author_sort | Pérez de Diego, Ana Cristina |
collection | PubMed |
description | The protective immune response generated by a commercial monovalent inactivated vaccine against bluetongue virus serotype 1 (BTV1) was studied. Five sheep were vaccinated, boost-vaccinated, and then challenged against BTV1 ALG/2006. RT-PCR did not detect viremia at any time during the experiment. Except a temperature increase observed after the initial and boost vaccinations, no clinical signs or lesions were observed. A specific and protective antibody response checked by ELISA was induced after vaccination and boost vaccination. This specific antibody response was associated with a significant increase in B lymphocytes confirmed by flow cytometry, while significant increases were not observed in T lymphocyte subpopulations (CD4(+), CD8(+), and WC1(+)), CD25(+) regulatory cells, or CD14(+) monocytes. After challenge with BTV1, the antibody response was much higher than during the boost vaccination period, and it was associated with a significant increase in B lymphocytes, CD14(+) monocytes, CD25(+) regulatory cells, and CD8(+) cytotoxic T lymphocytes. |
format | Online Article Text |
id | pubmed-3349316 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | The Scientific World Journal |
record_format | MEDLINE/PubMed |
spelling | pubmed-33493162012-05-22 Characterization of the Immune Response Induced by a Commercially Available Inactivated Bluetongue Virus Serotype 1 Vaccine in Sheep Pérez de Diego, Ana Cristina Sánchez-Cordón, Pedro José de las Heras, Ana Isabel Sánchez-Vizcaíno, José Manuel ScientificWorldJournal Research Article The protective immune response generated by a commercial monovalent inactivated vaccine against bluetongue virus serotype 1 (BTV1) was studied. Five sheep were vaccinated, boost-vaccinated, and then challenged against BTV1 ALG/2006. RT-PCR did not detect viremia at any time during the experiment. Except a temperature increase observed after the initial and boost vaccinations, no clinical signs or lesions were observed. A specific and protective antibody response checked by ELISA was induced after vaccination and boost vaccination. This specific antibody response was associated with a significant increase in B lymphocytes confirmed by flow cytometry, while significant increases were not observed in T lymphocyte subpopulations (CD4(+), CD8(+), and WC1(+)), CD25(+) regulatory cells, or CD14(+) monocytes. After challenge with BTV1, the antibody response was much higher than during the boost vaccination period, and it was associated with a significant increase in B lymphocytes, CD14(+) monocytes, CD25(+) regulatory cells, and CD8(+) cytotoxic T lymphocytes. The Scientific World Journal 2012-04-24 /pmc/articles/PMC3349316/ /pubmed/22619592 http://dx.doi.org/10.1100/2012/147158 Text en Copyright © 2012 Ana Cristina Pérez de Diego et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Pérez de Diego, Ana Cristina Sánchez-Cordón, Pedro José de las Heras, Ana Isabel Sánchez-Vizcaíno, José Manuel Characterization of the Immune Response Induced by a Commercially Available Inactivated Bluetongue Virus Serotype 1 Vaccine in Sheep |
title | Characterization of the Immune Response Induced by a Commercially Available Inactivated Bluetongue Virus Serotype 1 Vaccine in Sheep |
title_full | Characterization of the Immune Response Induced by a Commercially Available Inactivated Bluetongue Virus Serotype 1 Vaccine in Sheep |
title_fullStr | Characterization of the Immune Response Induced by a Commercially Available Inactivated Bluetongue Virus Serotype 1 Vaccine in Sheep |
title_full_unstemmed | Characterization of the Immune Response Induced by a Commercially Available Inactivated Bluetongue Virus Serotype 1 Vaccine in Sheep |
title_short | Characterization of the Immune Response Induced by a Commercially Available Inactivated Bluetongue Virus Serotype 1 Vaccine in Sheep |
title_sort | characterization of the immune response induced by a commercially available inactivated bluetongue virus serotype 1 vaccine in sheep |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3349316/ https://www.ncbi.nlm.nih.gov/pubmed/22619592 http://dx.doi.org/10.1100/2012/147158 |
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