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Characterization of the Immune Response Induced by a Commercially Available Inactivated Bluetongue Virus Serotype 1 Vaccine in Sheep

The protective immune response generated by a commercial monovalent inactivated vaccine against bluetongue virus serotype 1 (BTV1) was studied. Five sheep were vaccinated, boost-vaccinated, and then challenged against BTV1 ALG/2006. RT-PCR did not detect viremia at any time during the experiment. Ex...

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Autores principales: Pérez de Diego, Ana Cristina, Sánchez-Cordón, Pedro José, de las Heras, Ana Isabel, Sánchez-Vizcaíno, José Manuel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Scientific World Journal 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3349316/
https://www.ncbi.nlm.nih.gov/pubmed/22619592
http://dx.doi.org/10.1100/2012/147158
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author Pérez de Diego, Ana Cristina
Sánchez-Cordón, Pedro José
de las Heras, Ana Isabel
Sánchez-Vizcaíno, José Manuel
author_facet Pérez de Diego, Ana Cristina
Sánchez-Cordón, Pedro José
de las Heras, Ana Isabel
Sánchez-Vizcaíno, José Manuel
author_sort Pérez de Diego, Ana Cristina
collection PubMed
description The protective immune response generated by a commercial monovalent inactivated vaccine against bluetongue virus serotype 1 (BTV1) was studied. Five sheep were vaccinated, boost-vaccinated, and then challenged against BTV1 ALG/2006. RT-PCR did not detect viremia at any time during the experiment. Except a temperature increase observed after the initial and boost vaccinations, no clinical signs or lesions were observed. A specific and protective antibody response checked by ELISA was induced after vaccination and boost vaccination. This specific antibody response was associated with a significant increase in B lymphocytes confirmed by flow cytometry, while significant increases were not observed in T lymphocyte subpopulations (CD4(+), CD8(+), and WC1(+)), CD25(+) regulatory cells, or CD14(+) monocytes. After challenge with BTV1, the antibody response was much higher than during the boost vaccination period, and it was associated with a significant increase in B lymphocytes, CD14(+) monocytes, CD25(+) regulatory cells, and CD8(+) cytotoxic T lymphocytes.
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spelling pubmed-33493162012-05-22 Characterization of the Immune Response Induced by a Commercially Available Inactivated Bluetongue Virus Serotype 1 Vaccine in Sheep Pérez de Diego, Ana Cristina Sánchez-Cordón, Pedro José de las Heras, Ana Isabel Sánchez-Vizcaíno, José Manuel ScientificWorldJournal Research Article The protective immune response generated by a commercial monovalent inactivated vaccine against bluetongue virus serotype 1 (BTV1) was studied. Five sheep were vaccinated, boost-vaccinated, and then challenged against BTV1 ALG/2006. RT-PCR did not detect viremia at any time during the experiment. Except a temperature increase observed after the initial and boost vaccinations, no clinical signs or lesions were observed. A specific and protective antibody response checked by ELISA was induced after vaccination and boost vaccination. This specific antibody response was associated with a significant increase in B lymphocytes confirmed by flow cytometry, while significant increases were not observed in T lymphocyte subpopulations (CD4(+), CD8(+), and WC1(+)), CD25(+) regulatory cells, or CD14(+) monocytes. After challenge with BTV1, the antibody response was much higher than during the boost vaccination period, and it was associated with a significant increase in B lymphocytes, CD14(+) monocytes, CD25(+) regulatory cells, and CD8(+) cytotoxic T lymphocytes. The Scientific World Journal 2012-04-24 /pmc/articles/PMC3349316/ /pubmed/22619592 http://dx.doi.org/10.1100/2012/147158 Text en Copyright © 2012 Ana Cristina Pérez de Diego et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Pérez de Diego, Ana Cristina
Sánchez-Cordón, Pedro José
de las Heras, Ana Isabel
Sánchez-Vizcaíno, José Manuel
Characterization of the Immune Response Induced by a Commercially Available Inactivated Bluetongue Virus Serotype 1 Vaccine in Sheep
title Characterization of the Immune Response Induced by a Commercially Available Inactivated Bluetongue Virus Serotype 1 Vaccine in Sheep
title_full Characterization of the Immune Response Induced by a Commercially Available Inactivated Bluetongue Virus Serotype 1 Vaccine in Sheep
title_fullStr Characterization of the Immune Response Induced by a Commercially Available Inactivated Bluetongue Virus Serotype 1 Vaccine in Sheep
title_full_unstemmed Characterization of the Immune Response Induced by a Commercially Available Inactivated Bluetongue Virus Serotype 1 Vaccine in Sheep
title_short Characterization of the Immune Response Induced by a Commercially Available Inactivated Bluetongue Virus Serotype 1 Vaccine in Sheep
title_sort characterization of the immune response induced by a commercially available inactivated bluetongue virus serotype 1 vaccine in sheep
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3349316/
https://www.ncbi.nlm.nih.gov/pubmed/22619592
http://dx.doi.org/10.1100/2012/147158
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