One-step generation of error-prone PCR libraries using Gateway(® )technology

BACKGROUND: Error-prone PCR (epPCR) libraries are one of the tools used in directed evolution. The Gateway(® )technology allows constructing epPCR libraries virtually devoid of any background (i.e., of insert-free plasmid), but requires two steps: the BP and the LR reactions and the associated E. co...

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Detalles Bibliográficos
Autores principales: Gruet, Antoine, Longhi, Sonia, Bignon, Christophe
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2012
Materias:
Technical Notes
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3349575/
https://www.ncbi.nlm.nih.gov/pubmed/22289297
http://dx.doi.org/10.1186/1475-2859-11-14

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3349575/
https://www.ncbi.nlm.nih.gov/pubmed/22289297
http://dx.doi.org/10.1186/1475-2859-11-14

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