Cargando…
One-step generation of error-prone PCR libraries using Gateway(® )technology
BACKGROUND: Error-prone PCR (epPCR) libraries are one of the tools used in directed evolution. The Gateway(® )technology allows constructing epPCR libraries virtually devoid of any background (i.e., of insert-free plasmid), but requires two steps: the BP and the LR reactions and the associated E. co...
| Autores principales: | , , |
|---|---|
| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
BioMed Central
2012
|
| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3349575/ https://www.ncbi.nlm.nih.gov/pubmed/22289297 http://dx.doi.org/10.1186/1475-2859-11-14 |