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Increased CDC20 expression is associated with pancreatic ductal adenocarcinoma differentiation and progression

PURPOSE: Cell division cycle 20 (CDC20) homolog is an anaphase-promoting complex activator that is essential for cell division, but whether its expression in pancreatic ductal adenocarcinoma (PDAC) is significant is unknown. In this retrospective study, we determined whether aberrant CDC20 expressio...

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Autores principales: Chang, David Z, Ma, Ying, Ji, Baoan, Liu, Yan, Hwu, Patrick, Abbruzzese, James L, Logsdon, Craig, Wang, Huamin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3350393/
https://www.ncbi.nlm.nih.gov/pubmed/22475564
http://dx.doi.org/10.1186/1756-8722-5-15
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author Chang, David Z
Ma, Ying
Ji, Baoan
Liu, Yan
Hwu, Patrick
Abbruzzese, James L
Logsdon, Craig
Wang, Huamin
author_facet Chang, David Z
Ma, Ying
Ji, Baoan
Liu, Yan
Hwu, Patrick
Abbruzzese, James L
Logsdon, Craig
Wang, Huamin
author_sort Chang, David Z
collection PubMed
description PURPOSE: Cell division cycle 20 (CDC20) homolog is an anaphase-promoting complex activator that is essential for cell division, but whether its expression in pancreatic ductal adenocarcinoma (PDAC) is significant is unknown. In this retrospective study, we determined whether aberrant CDC20 expression can be used as a biomarker in pancreatic ductal adenocarcinoma (PDAC) tumorigenesis and whether its expression reflects clinical progression. EXPERIMENTAL DESIGN: We compared CDC20 expression levels in normal, cancerous, and inflamed pancreatic tissues from stage II PDAC patients with clinical outcomes and determined CDC20 levels in seven PDAC cell lines. CDC20 was identified using a cDNA microarray database containing gene expression profiles for PDAC tissues and cell lines and chronic pancreatitis and normal pancreas tissues. Its expression was confirmed by real-time quantitative reverse-transcriptase-polymerase chain reaction (qRT-PCR). An immunohistochemical analysis of tissue microarrays from resected PDAC tumors and paired benign pancreatic tissues was done and CDC20 levels were correlated with clinical outcome. RESULTS: Fifty-six patients were included in this study. A microarray analysis revealed 5-fold higher CDC20 expression in PDAC tissue than in chronic pancreatitis tissue. A qRT-PCR analysis confirmed a mean 20-fold higher CDC20 level in PDAC tissue than in normal pancreas and pancreatitis tissue. RNA and protein CDC20 expression was detected in several PDAC cell lines. An immunohistochemical analysis revealed higher CDC20 protein expression levels in PDAC tissue than in normal pancreas tissue, and high CDC20 expression was associated with poor differentiation (P = 0.020) and a significantly lower 5-year recurrence-free survival rate (P = 0.039); we also found a trend toward a shorter overall survival duration. CONCLUSIONS: Aberrant CDC20 expression may play an important role in PDAC tumorigenesis and progression and may thus be useful as a marker of disease progression and prognosis and as a therapeutic target.
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spelling pubmed-33503932012-05-12 Increased CDC20 expression is associated with pancreatic ductal adenocarcinoma differentiation and progression Chang, David Z Ma, Ying Ji, Baoan Liu, Yan Hwu, Patrick Abbruzzese, James L Logsdon, Craig Wang, Huamin J Hematol Oncol Research PURPOSE: Cell division cycle 20 (CDC20) homolog is an anaphase-promoting complex activator that is essential for cell division, but whether its expression in pancreatic ductal adenocarcinoma (PDAC) is significant is unknown. In this retrospective study, we determined whether aberrant CDC20 expression can be used as a biomarker in pancreatic ductal adenocarcinoma (PDAC) tumorigenesis and whether its expression reflects clinical progression. EXPERIMENTAL DESIGN: We compared CDC20 expression levels in normal, cancerous, and inflamed pancreatic tissues from stage II PDAC patients with clinical outcomes and determined CDC20 levels in seven PDAC cell lines. CDC20 was identified using a cDNA microarray database containing gene expression profiles for PDAC tissues and cell lines and chronic pancreatitis and normal pancreas tissues. Its expression was confirmed by real-time quantitative reverse-transcriptase-polymerase chain reaction (qRT-PCR). An immunohistochemical analysis of tissue microarrays from resected PDAC tumors and paired benign pancreatic tissues was done and CDC20 levels were correlated with clinical outcome. RESULTS: Fifty-six patients were included in this study. A microarray analysis revealed 5-fold higher CDC20 expression in PDAC tissue than in chronic pancreatitis tissue. A qRT-PCR analysis confirmed a mean 20-fold higher CDC20 level in PDAC tissue than in normal pancreas and pancreatitis tissue. RNA and protein CDC20 expression was detected in several PDAC cell lines. An immunohistochemical analysis revealed higher CDC20 protein expression levels in PDAC tissue than in normal pancreas tissue, and high CDC20 expression was associated with poor differentiation (P = 0.020) and a significantly lower 5-year recurrence-free survival rate (P = 0.039); we also found a trend toward a shorter overall survival duration. CONCLUSIONS: Aberrant CDC20 expression may play an important role in PDAC tumorigenesis and progression and may thus be useful as a marker of disease progression and prognosis and as a therapeutic target. BioMed Central 2012-04-04 /pmc/articles/PMC3350393/ /pubmed/22475564 http://dx.doi.org/10.1186/1756-8722-5-15 Text en Copyright ©2012 Chang et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Chang, David Z
Ma, Ying
Ji, Baoan
Liu, Yan
Hwu, Patrick
Abbruzzese, James L
Logsdon, Craig
Wang, Huamin
Increased CDC20 expression is associated with pancreatic ductal adenocarcinoma differentiation and progression
title Increased CDC20 expression is associated with pancreatic ductal adenocarcinoma differentiation and progression
title_full Increased CDC20 expression is associated with pancreatic ductal adenocarcinoma differentiation and progression
title_fullStr Increased CDC20 expression is associated with pancreatic ductal adenocarcinoma differentiation and progression
title_full_unstemmed Increased CDC20 expression is associated with pancreatic ductal adenocarcinoma differentiation and progression
title_short Increased CDC20 expression is associated with pancreatic ductal adenocarcinoma differentiation and progression
title_sort increased cdc20 expression is associated with pancreatic ductal adenocarcinoma differentiation and progression
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3350393/
https://www.ncbi.nlm.nih.gov/pubmed/22475564
http://dx.doi.org/10.1186/1756-8722-5-15
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