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Dcc Regulates Asymmetric Outgrowth of Forebrain Neurons in Zebrafish
The guidance receptor DCC (deleted in colorectal cancer) ortholog UNC-40 regulates neuronal asymmetry development in Caenorhabditis elegans, but it is not known whether DCC plays a role in the specification of neuronal polarity in vertebrates. To examine the roles of DCC in neuronal asymmetry regula...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3351449/ https://www.ncbi.nlm.nih.gov/pubmed/22606267 http://dx.doi.org/10.1371/journal.pone.0036516 |
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author | Gao, Jingxia Zhang, Changwen Yang, Bin Sun, Liu Zhang, Cuizhen Westerfield, Monte Peng, Gang |
author_facet | Gao, Jingxia Zhang, Changwen Yang, Bin Sun, Liu Zhang, Cuizhen Westerfield, Monte Peng, Gang |
author_sort | Gao, Jingxia |
collection | PubMed |
description | The guidance receptor DCC (deleted in colorectal cancer) ortholog UNC-40 regulates neuronal asymmetry development in Caenorhabditis elegans, but it is not known whether DCC plays a role in the specification of neuronal polarity in vertebrates. To examine the roles of DCC in neuronal asymmetry regulation in vertebrates, we studied zebrafish anterior dorsal telencephalon (ADt) neuronal axons. We generated transgenic zebrafish animals expressing the photo-convertible fluorescent protein Kaede in ADt neurons and then photo-converted Kaede to label specifically the ADt neuron axons. We found that ADt axons normally project ventrally. Knock down of Dcc function by injecting antisense morpholino oligonucleotides caused the ADt neurons to project axons dorsally. To examine the axon projection pattern of individual ADt neurons, we labeled single ADt neurons using a forebrain-specific promoter to drive fluorescent protein expression. We found that individual ADt neurons projected axons dorsally or formed multiple processes after morpholino knock down of Dcc function. We further found that knock down of the Dcc ligand, Netrin1, also caused ADt neurons to project axons dorsally. Knockdown of Neogenin1, a guidance receptor closely related to Dcc, enhanced the formation of aberrant dorsal axons in embryos injected with Dcc morpholino. These experiments provide the first evidence that Dcc regulates polarized axon initiation and asymmetric outgrowth of forebrain neurons in vertebrates. |
format | Online Article Text |
id | pubmed-3351449 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-33514492012-05-17 Dcc Regulates Asymmetric Outgrowth of Forebrain Neurons in Zebrafish Gao, Jingxia Zhang, Changwen Yang, Bin Sun, Liu Zhang, Cuizhen Westerfield, Monte Peng, Gang PLoS One Research Article The guidance receptor DCC (deleted in colorectal cancer) ortholog UNC-40 regulates neuronal asymmetry development in Caenorhabditis elegans, but it is not known whether DCC plays a role in the specification of neuronal polarity in vertebrates. To examine the roles of DCC in neuronal asymmetry regulation in vertebrates, we studied zebrafish anterior dorsal telencephalon (ADt) neuronal axons. We generated transgenic zebrafish animals expressing the photo-convertible fluorescent protein Kaede in ADt neurons and then photo-converted Kaede to label specifically the ADt neuron axons. We found that ADt axons normally project ventrally. Knock down of Dcc function by injecting antisense morpholino oligonucleotides caused the ADt neurons to project axons dorsally. To examine the axon projection pattern of individual ADt neurons, we labeled single ADt neurons using a forebrain-specific promoter to drive fluorescent protein expression. We found that individual ADt neurons projected axons dorsally or formed multiple processes after morpholino knock down of Dcc function. We further found that knock down of the Dcc ligand, Netrin1, also caused ADt neurons to project axons dorsally. Knockdown of Neogenin1, a guidance receptor closely related to Dcc, enhanced the formation of aberrant dorsal axons in embryos injected with Dcc morpholino. These experiments provide the first evidence that Dcc regulates polarized axon initiation and asymmetric outgrowth of forebrain neurons in vertebrates. Public Library of Science 2012-05-14 /pmc/articles/PMC3351449/ /pubmed/22606267 http://dx.doi.org/10.1371/journal.pone.0036516 Text en Gao et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Gao, Jingxia Zhang, Changwen Yang, Bin Sun, Liu Zhang, Cuizhen Westerfield, Monte Peng, Gang Dcc Regulates Asymmetric Outgrowth of Forebrain Neurons in Zebrafish |
title | Dcc Regulates Asymmetric Outgrowth of Forebrain Neurons in Zebrafish |
title_full | Dcc Regulates Asymmetric Outgrowth of Forebrain Neurons in Zebrafish |
title_fullStr | Dcc Regulates Asymmetric Outgrowth of Forebrain Neurons in Zebrafish |
title_full_unstemmed | Dcc Regulates Asymmetric Outgrowth of Forebrain Neurons in Zebrafish |
title_short | Dcc Regulates Asymmetric Outgrowth of Forebrain Neurons in Zebrafish |
title_sort | dcc regulates asymmetric outgrowth of forebrain neurons in zebrafish |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3351449/ https://www.ncbi.nlm.nih.gov/pubmed/22606267 http://dx.doi.org/10.1371/journal.pone.0036516 |
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