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Methylglyoxal Mediates Adipocyte Proliferation by Increasing Phosphorylation of Akt1
Methylglyoxal (MG) is a highly reactive metabolite physiologically presented in all biological systems. The effects of MG on diabetes and hypertension have been long recognized. In the present study, we investigated the potential role of MG in obesity, one of the most important factors to cause meta...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3351465/ https://www.ncbi.nlm.nih.gov/pubmed/22606274 http://dx.doi.org/10.1371/journal.pone.0036610 |
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author | Jia, Xuming Chang, Tuanjie Wilson, Thomas W. Wu, Lingyun |
author_facet | Jia, Xuming Chang, Tuanjie Wilson, Thomas W. Wu, Lingyun |
author_sort | Jia, Xuming |
collection | PubMed |
description | Methylglyoxal (MG) is a highly reactive metabolite physiologically presented in all biological systems. The effects of MG on diabetes and hypertension have been long recognized. In the present study, we investigated the potential role of MG in obesity, one of the most important factors to cause metabolic syndrome. An increased MG accumulation was observed in the adipose tissue of obese Zucker rats. Cell proliferation assay showed that 5–20 µM of MG stimulated the proliferation of 3T3-L1 cells. Further study suggested that accumulated-MG stimulated the phosphorylation of Akt1 and its targets including p21 and p27. The activated Akt1 then increased the activity of CDK2 and accelerated the cell cycle progression of 3T3-L1 cells. The effects of MG were efficiently reversed by advanced glycation end product (AGE) breaker alagebrium and Akt inhibitor SH-6. In summary, our study revealed a previously unrecognized effect of MG in stimulating adipogenesis by up-regulation of Akt signaling pathway and this mechanism might offer a new approach to explain the development of obesity. |
format | Online Article Text |
id | pubmed-3351465 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-33514652012-05-17 Methylglyoxal Mediates Adipocyte Proliferation by Increasing Phosphorylation of Akt1 Jia, Xuming Chang, Tuanjie Wilson, Thomas W. Wu, Lingyun PLoS One Research Article Methylglyoxal (MG) is a highly reactive metabolite physiologically presented in all biological systems. The effects of MG on diabetes and hypertension have been long recognized. In the present study, we investigated the potential role of MG in obesity, one of the most important factors to cause metabolic syndrome. An increased MG accumulation was observed in the adipose tissue of obese Zucker rats. Cell proliferation assay showed that 5–20 µM of MG stimulated the proliferation of 3T3-L1 cells. Further study suggested that accumulated-MG stimulated the phosphorylation of Akt1 and its targets including p21 and p27. The activated Akt1 then increased the activity of CDK2 and accelerated the cell cycle progression of 3T3-L1 cells. The effects of MG were efficiently reversed by advanced glycation end product (AGE) breaker alagebrium and Akt inhibitor SH-6. In summary, our study revealed a previously unrecognized effect of MG in stimulating adipogenesis by up-regulation of Akt signaling pathway and this mechanism might offer a new approach to explain the development of obesity. Public Library of Science 2012-05-14 /pmc/articles/PMC3351465/ /pubmed/22606274 http://dx.doi.org/10.1371/journal.pone.0036610 Text en Jia et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Jia, Xuming Chang, Tuanjie Wilson, Thomas W. Wu, Lingyun Methylglyoxal Mediates Adipocyte Proliferation by Increasing Phosphorylation of Akt1 |
title | Methylglyoxal Mediates Adipocyte Proliferation by Increasing Phosphorylation of Akt1 |
title_full | Methylglyoxal Mediates Adipocyte Proliferation by Increasing Phosphorylation of Akt1 |
title_fullStr | Methylglyoxal Mediates Adipocyte Proliferation by Increasing Phosphorylation of Akt1 |
title_full_unstemmed | Methylglyoxal Mediates Adipocyte Proliferation by Increasing Phosphorylation of Akt1 |
title_short | Methylglyoxal Mediates Adipocyte Proliferation by Increasing Phosphorylation of Akt1 |
title_sort | methylglyoxal mediates adipocyte proliferation by increasing phosphorylation of akt1 |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3351465/ https://www.ncbi.nlm.nih.gov/pubmed/22606274 http://dx.doi.org/10.1371/journal.pone.0036610 |
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