Novel protein extraction approach using micro-sized chamber for evaluation of proteins eluted from formalin-fixed paraffin-embedded tissue sections

We describe a novel antigen-retrieval method using a micro-sized chamber for mass spectrometry (MS) analysis to identify proteins that are preferentially eluted from formalin-fixed paraffin-embedded (FFPE) samples. This approach revealed that heat-induced antigen retrieval (HIAR) from an FFPE sample...

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Autores principales: Hatakeyama, Keiichi, Wakabayashi-Nakao, Kanako, Aoki, Yutaka, Ogura, Shun-ichiro, Yamaguchi, Ken, Nakajima, Takashi, Sato, Taka-Aki, Mochizuki, Tohru, Hayashi, Isamu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2012
Materias:
Methodology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3352043/
https://www.ncbi.nlm.nih.gov/pubmed/22439923
http://dx.doi.org/10.1186/1477-5956-10-19
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author Hatakeyama, Keiichi
Wakabayashi-Nakao, Kanako
Aoki, Yutaka
Ogura, Shun-ichiro
Yamaguchi, Ken
Nakajima, Takashi
Sato, Taka-Aki
Mochizuki, Tohru
Hayashi, Isamu
author_facet Hatakeyama, Keiichi
Wakabayashi-Nakao, Kanako
Aoki, Yutaka
Ogura, Shun-ichiro
Yamaguchi, Ken
Nakajima, Takashi
Sato, Taka-Aki
Mochizuki, Tohru
Hayashi, Isamu
author_sort Hatakeyama, Keiichi
collection PubMed
description We describe a novel antigen-retrieval method using a micro-sized chamber for mass spectrometry (MS) analysis to identify proteins that are preferentially eluted from formalin-fixed paraffin-embedded (FFPE) samples. This approach revealed that heat-induced antigen retrieval (HIAR) from an FFPE sample fixed on a glass slide not only improves protein identification, but also facilitates preferential elution of protein subsets corresponding to the properties of antigen-retrieval buffers. Our approach may contribute to an understanding of the mechanism of HIAR.
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spelling pubmed-33520432012-05-16 Novel protein extraction approach using micro-sized chamber for evaluation of proteins eluted from formalin-fixed paraffin-embedded tissue sections Hatakeyama, Keiichi Wakabayashi-Nakao, Kanako Aoki, Yutaka Ogura, Shun-ichiro Yamaguchi, Ken Nakajima, Takashi Sato, Taka-Aki Mochizuki, Tohru Hayashi, Isamu Proteome Sci Methodology We describe a novel antigen-retrieval method using a micro-sized chamber for mass spectrometry (MS) analysis to identify proteins that are preferentially eluted from formalin-fixed paraffin-embedded (FFPE) samples. This approach revealed that heat-induced antigen retrieval (HIAR) from an FFPE sample fixed on a glass slide not only improves protein identification, but also facilitates preferential elution of protein subsets corresponding to the properties of antigen-retrieval buffers. Our approach may contribute to an understanding of the mechanism of HIAR. BioMed Central 2012-03-23 /pmc/articles/PMC3352043/ /pubmed/22439923 http://dx.doi.org/10.1186/1477-5956-10-19 Text en Copyright ©2012 Hatakeyama et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Methodology
Hatakeyama, Keiichi
Wakabayashi-Nakao, Kanako
Aoki, Yutaka
Ogura, Shun-ichiro
Yamaguchi, Ken
Nakajima, Takashi
Sato, Taka-Aki
Mochizuki, Tohru
Hayashi, Isamu
Novel protein extraction approach using micro-sized chamber for evaluation of proteins eluted from formalin-fixed paraffin-embedded tissue sections
title Novel protein extraction approach using micro-sized chamber for evaluation of proteins eluted from formalin-fixed paraffin-embedded tissue sections
title_full Novel protein extraction approach using micro-sized chamber for evaluation of proteins eluted from formalin-fixed paraffin-embedded tissue sections
title_fullStr Novel protein extraction approach using micro-sized chamber for evaluation of proteins eluted from formalin-fixed paraffin-embedded tissue sections
title_full_unstemmed Novel protein extraction approach using micro-sized chamber for evaluation of proteins eluted from formalin-fixed paraffin-embedded tissue sections
title_short Novel protein extraction approach using micro-sized chamber for evaluation of proteins eluted from formalin-fixed paraffin-embedded tissue sections
title_sort novel protein extraction approach using micro-sized chamber for evaluation of proteins eluted from formalin-fixed paraffin-embedded tissue sections
topic Methodology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3352043/
https://www.ncbi.nlm.nih.gov/pubmed/22439923
http://dx.doi.org/10.1186/1477-5956-10-19
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